# US 10,501,560 B2 · Low-viscosity starch hydrolysate with improved retrogradation behaviour

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- Number: US 10,501,560 B2
- Document ID: US10501560B2
- Status: Granted
- Kind: B2
- Application number: 15/781,251
- Filed: 2016-12-12
- Published: 2018-11-08
- Granted: 2019-12-10
- Earliest claimed: 2015-12-10
- Assignees: Roquette Freres
- Applicants: Roquette Freres
- Inventors: Mathias Ibert; Aline Lecocq; Pierre Lanos
- Web page: https://patentgrep.com/patent/US10501560B2

## CPC

- C08B 30/18: Dextrin {, e.g. yellow canari, white dextrin, amylodextrin or maltodextrin; Methods of depolymerisation, e.g. by irradiation or mechanically}
- A23L 29/212: Starch; Modified starch; Starch derivatives, e.g. esters or ethers (containing starch hydrolysates, e.g. dextrin, A23L29/30)
- A23L 29/35: {Degradation products of starch, e.g. hydrolysates, dextrins; Enzymatically modified starches}
- C08L 3/02: Starch; Degradation products thereof, e.g. dextrin
- C12P 19/14: produced by the action of a carbohydrase {(EC 3.2.x)}, e.g. by alpha-amylase {, e.g. by cellulase, hemicellulase}
- A61K 47/26: Carbohydrates, e.g. sugar alcohols, amino sugars, nucleic acids, mono-, di- or oligo-saccharides; Derivatives thereof, e.g. polysorbates, sorbitan fatty acid esters or glycyrrhizin
- A61K 47/36: Polysaccharides; Derivatives thereof, e.g. gums, starch, alginate, dextrin, hyaluronic acid, chitosan, inulin, agar or pectin
- C08B: POLYSACCHARIDES; DERIVATIVES THEREOF (polysaccharides containing less than six saccharide radicals attached to each other by glycosidic linkages C07H; fermentation or enzyme-using processes C12P19/00; sugar industry C13; production of cellulose D21)
- A23L: FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF (preservation thereof A23B)
- C08L: COMPOSITIONS OF MACROMOLECULAR COMPOUNDS (compositions based on polymerisable monomers C08F, C08G; artificial filaments or fibres D01F; textile treating compositions D06)
- C12P: FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {(brewing of beer C12C; producing vinegar C12J; producing specific peptides or proteins C07K; producing enzymes C12N9/00; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification C12N15/00; measuring or testing processes involving enzymes or microorganisms C12Q; measuring or testing processes involving nucleic acid amplification reactions C12Q1/6844; fermentation processes to form a food composition, A21 or A23; compounds in general, see the relevant compound class, e.g. C01, C07)}
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)

## Abstract

The invention relates to a starch hydrolysate having a Dextrose Equivalent DE of between 5 and 30 and a specific carbohydrate profile. In particular, the hydrolysate of the invention has improved retrogradation behaviour. The invention also relates to a method for the production of said starch hydrolysate.

## Claims

### Claim 1

A starch hydrolysate having a Dextrose Equivalent DE ranging from 5 to 30 and in which the DE, the dry weight content of saccharides having a degree of polymerization ranging from 10 to 20 (DP 10-20) and the dry weight content of saccharides having a degree of polymerization of 50 or more (DP 50+) are such that they meet the following inequations:
−0.83×DE+25≤% DP50+≤−1.07×DE+40;
−0.83×DE+27.5≤% DP10-20≤−1.25×DE+55.

### Claim 2 (depends on 1)

The starch hydrolysate as claimed in claim 1, having:
a DE ranging from 24 to 30;
a dry weight content of DP 10-20 ranging from 10% to 18%;
a dry weight content of DP 50+ ranging from 3% to 13%.

### Claim 3 (depends on 1)

The starch hydrolysate as claimed in claim 1, having:
a DE ranging from 20 to 24;
a dry weight content of DP 10-20 ranging from 10% to 20%;
a dry weight content of DP 50+ranging from 6% to 18%.

### Claim 4 (depends on 1)

The starch hydrolysate as claimed in claim 1, having:
a DE ranging from 17 to 20;
a dry weight content of DP 10-20 ranging from 12% to 25%;
a dry weight content of DP 50+ ranging from 10% to 19%.

### Claim 5 (depends on 1)

The starch hydrolysate as claimed in claim 1, having:
a DE ranging from 15 to 17;
a dry weight content of DP 10-20 ranging from 16% to 27%;
a dry weight content of DP 50+ ranging from 13% to 22%.

### Claim 6 (depends on 1)

The starch hydrolysate as claimed in claim 1, having:
a DE ranging from 13 to 15;
a dry weight content of DP 10-20 ranging from 17% to 30%;
a dry weight content of DP 50+ ranging from 17% to 25%.

### Claim 7 (depends on 1)

The starch hydrolysate as claimed in claim 1, having:
a DE ranging from 10 to 13;
a dry weight content of DP 10-20 ranging from 20% to 32%;
a dry weight content of DP 50+ ranging from 18% to 28%.

### Claim 8 (depends on 1)

The hydrolysate as claimed in claim 1, characterized in that it is in the form of an aqueous solution.

### Claim 9 (depends on 1)

The hydrolysate as claimed in claim 1, characterized in that it is in the form of a powder.

### Claim 10 (depends on 1)

A process for producing the starch hydrolysate according to claim 1, comprising:
a) a step of bringing an aqueous solution of starch or of starchy material into contact with at least one alpha-amylase comprising an Enzyme V;
b) followed by a step of hydrolysis of said aqueous solution;
c) followed by a step of inhibition of the Enzyme V so as to form a solution of intermediate hydrolysate having a DE equal to DEc;
d) followed by a step of bringing the solution of intermediate hydrolysate obtained in step c) into contact with at least one additional alpha-amylase, different than the Enzyme V;
e) followed by a second hydrolysis step so as to form a hydrolysate for a period of time sufficient for it to have a DEe ranging from 5 to 30;
f) followed by a step of recovering the hydrolysate formed in step e); characterized in that the Enzyme V is an enzyme chosen from:
polypeptides encoded by a nucleotide sequence as represented in SEQ ID NO1 or a nucleotide sequence having a percentage identity relative to the sequence SEQ ID NO1 at least equal to 80%, or
polypeptides comprising the enzymatically active fragment of the nucleotide sequence encoded by the sequence SEQ ID NO1.

### Claim 11 (depends on 10)

The process as claimed in claim 10, characterized in that the aqueous solution of starch formed in step a) has a pH ranging from 4.3 to 5.9.

### Claim 12 (depends on 10)

The process as claimed in claim 10, characterized in that the solution of hydrolysate formed in step d) has a pH ranging from 4.3 to 5.9.

### Claim 13 (depends on 10)

The process as claimed in claim 10, characterized in that the inhibition step is carried out by heating the solution of intermediate hydrolysate at a temperature greater than or equal to 150° C.

### Claim 14 (depends on 10)

The process as claimed in claim 10, characterized in that the DEe is greater than or equal to DEc+3.

### Claim 15 (depends on 10)

The process as claimed in claim 10, characterized in that it also comprises at least one step g) of converting the hydrolysate recovered in step f).

### Claim 16 (depends on 15)

The process as claimed in claim 15, characterized in that step g) is a step of forming the hydrolysate in powder form by means of a spray-drying step.

### Claim 17 (depends on 1)

A food product, comprising the starch hydrolysate as claimed in claim 1 and at least one food ingredient.

### Claim 18 (depends on 1)

A pharmaceutical product, comprising the starch hydrolysate as claimed in claim 1 and at least one pharmaceutical ingredient.

### Claim 19

A mixture, comprising the starch hydrolysate as claimed in claim land a second hydrolysate.

### Claim 20 (depends on 10)

The process according to claim 10, wherein the percentage identity is at least 85%.

### Claim 21 (depends on 10)

The process according to claim 10, wherein the percentage identity is at least 90%.

### Claim 22 (depends on 10)

The process according to claim 10, wherein the percentage identity is at least 98%.

### Claim 23 (depends on 10)

The process according to claim 10, wherein the percentage identity is at least 99%.