# US 10,501,508 B2 · Minimized cyanobacterial microcompartment for carbon dioxide fixation

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- Number: US 10,501,508 B2
- Document ID: US10501508B2
- Status: Granted
- Kind: B2
- Application number: 15/685,742
- Filed: 2017-08-24
- Published: 2018-03-01
- Granted: 2019-12-10
- Earliest claimed: 2016-08-24
- Assignees: Board of Trustees of Michigan State University
- Applicants: Board of Trustees of Michigan State University
- Inventors: Cheryl A. Kerfeld; Cesar R. Gonzalez
- Web page: https://patentgrep.com/patent/US10501508B2

## CPC

- C07K 14/405: from algae
- C07K 14/195: from bacteria
- C10L 1/02: essentially based on components consisting of carbon, hydrogen, and oxygen only
- C12N 1/32: Processes using, or culture media containing, lower alkanols, i.e. C1 to C6
- C12N 9/14: Hydrolases (3)
- C12N 15/8261: {with agronomic (input) traits, e.g. crop yield}
- C12P 5/007: {containing one or more isoprene units, i.e. terpenes (carotenes C12P23/00)}
- C12P 7/065: {with microorganisms other than yeasts}
- C12P 7/16: Butanols
- C12P 7/24: containing a carbonyl group
- C07K 2319/00: Fusion polypeptide
- C12P 39/00: Processes involving microorganisms of different genera in the same process, simultaneously
- Y02A 40/146: Genetically Modified [GMO] plants, e.g. transgenic plants
- Y02E 50/10: Biofuels, e.g. bio-diesel
- Y02P 60/20: Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)
- C10L: FUELS NOT OTHERWISE PROVIDED FOR; NATURAL GAS; SYNTHETIC NATURAL GAS OBTAINED BY PROCESSES NOT COVERED BY SUBCLASSES C10G OR C10K; LIQUIFIED PETROLEUM GAS; USE OF ADDITIVES TO FUELS OR FIRES; FIRE-LIGHTERS
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- C12P: FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {(brewing of beer C12C; producing vinegar C12J; producing specific peptides or proteins C07K; producing enzymes C12N9/00; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification C12N15/00; measuring or testing processes involving enzymes or microorganisms C12Q; measuring or testing processes involving nucleic acid amplification reactions C12Q1/6844; fermentation processes to form a food composition, A21 or A23; compounds in general, see the relevant compound class, e.g. C01, C07)}
- Y02A: TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02E: REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02P: CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS

## Abstract

A fusion chimeric protein is described herein that can assemble a functional carboxysome core, which is able to fix carbon by taking atmospheric carbon dioxide and converting it into useful carbon-containing compounds such as 3-phosphoglycerate (3-PGA).

## Claims

### Claim 1

A fusion protein comprising a polypeptide comprising at least two fused small subunit-like domains (SSLDs) that can bind or nucleate with ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco), each SSLD comprising at least 95% sequence identity to any of SEQ ID NO: 75, 76 or 77, one SSLD fused to at least one domain having carbonic anhydrase activity, and at least one encapsulation peptide that interacts or binds with to one or more carboxysome shell protein, each encapsulation peptide comprising at least 95% sequence identity to any of SEQ ID NO: 14, 15 or 16 fused to the at least one carbonic anhydrase domain.

### Claim 2 (depends on 1)

The fusion protein of claim 1, wherein the Rubisco can synthesize 3-phosphoglycerate (3-PGA).

### Claim 3 (depends on 1)

The fusion protein of claim 1, wherein the at least one carbonic anhydrase domain comprises at least 95% sequence identity to any of SEQ ID NO:17-21 or 71.