# US 10,500,412 B2 · Devices, systems, and methods for inducing dermal blood vessel leakage > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 10,500,412 B2 - Document ID: US10500412B2 - Status: Granted - Kind: B2 - Application number: 15/545,039 - Filed: 2016-01-22 - Published: 2018-01-11 - Granted: 2019-12-10 - Earliest claimed: 2015-01-22 - Assignees: The General Hospital Corporation - Applicants: The General Hospital Corporation - Inventors: Mei X. Wu - Web page: https://patentgrep.com/patent/US10500412B2 ## CPC - A61N 5/0616: {Skin treatment other than tanning} - A61B 5/145: Measuring characteristics of blood in vivo, e.g. gas concentration or pH-value {; Measuring characteristics of body fluids or tissues, e.g. interstitial fluid or cerebral tissue} (non-radiation detecting or locating of foreign bodies in blood A61B5/06) - A61B 5/14552: {Details of sensors specially adapted therefor} - A61B 5/14556: {by fluorescence (A61B5/14555 takes precedence)} - A61B 5/150022: {for capillary blood or interstitial fluid} - A61B 5/150061: {Means for enhancing collection} - A61B 5/150427: {Specific tip design, e.g. for improved penetration characteristics} - A61B 5/150969: {Low-profile devices which resemble patches or plasters, e.g. also allowing collection of blood samples for testing} - A61B 5/150984: {Microneedles or microblades} - A61B 5/15105: {Purely manual piercing, i.e. the user pierces the skin without the assistance of any driving means or driving devices} - A61B 5/15136: {by use of radiation, e.g. laser} - A61B 5/685: {Microneedles} - A61M 37/0015: {by using microneedles} - A61B 5/150068: {by tissue compression, e.g. with specially designed surface of device contacting the skin area to be pierced} - A61B 5/150076: {by heating} - A61M 2037/0007: {having means for enhancing the permeation of substances through the epidermis, e.g. using suction or depression, electric or magnetic fields, sound waves or chemical agents} - A61M 2037/0023: {Drug applicators using microneedles} - A61N 5/067: using laser light - A61N 5/1027: {Interstitial radiation therapy} - A61N 2005/1021: {Radioactive fluid} - A61N: ELECTROTHERAPY; MAGNETOTHERAPY; RADIATION THERAPY; ULTRASOUND THERAPY (measurement of bioelectric currents A61B; surgical instruments, devices or methods for transferring non-mechanical forms of energy to or from the body A61B18/00; anaesthetic apparatus in general A61M; incandescent lamps H01K; infrared radiators for heating H05B) - A61B: DIAGNOSIS; SURGERY; IDENTIFICATION (usefulness limited to only animals A61D) - A61M: DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY (introducing media into or onto the bodies of animals A61D7/00; means for inserting tampons A61F13/26; devices for administering food or medicines orally A61J; containers for collecting, storing or administering blood or medical fluids A61J1/05); DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY (surgery A61B; chemical aspects of surgical articles A61L); DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR {(Electrotherapy, e.g. producing anaesthesia by the use of alternating or intermittent currents A61N1/36021)} ## Abstract The present disclosure relates generally to devices, systems, and methods for diagnosis and treatment via laser-treated skin and, more particularly, to devices, systems, and methods for inducing leakage or rupture of one or more blood vessels comprising the dermis for various diagnostic and therapeutic applications. Other aspects of the present disclosure can include methods for detecting one or more target analytes in a dermis of a subject, methods for facilitating skin-to-blood delivery of agent in a subject, and methods for collecting a fluid sample from the dermis of a subject. ## Claims ### Claim 1 A device comprising: at least one penetration member having a body defined by oppositely disposed proximal and distal ends, the body having a length such that at least a portion of the distal end extends into a dermis of the skin of a subject when the device is contacted with the subject's skin; and a laser source coupled to the at least one penetration member so that, upon activation, the laser source is configured to deliver a laser beam into the dermis for a time sufficient to induce leakage or rupture of at least one blood vessel comprising the dermis; wherein at least a portion of the distal end is coated with one or more detection reagents for detecting one or more target analytes in the fluid leaked from the at least one blood vessel. ### Claim 2 (depends on 1) The device of claim 1, wherein the penetration member is a needle, a microneedle, or a microprojection comprising a microneedle array or a microprojection array. ### Claim 3 (depends on 1) The device of claim 1, wherein the penetration member is hollow and the laser source is coupled to an optical fiber that is at least partly disposed within the hollow penetration member. ### Claim 4 (depends on 1) The device of claim 1, wherein the penetration member is made of a solid, transparent material such that the laser beam is configured to penetrate into the dermis via the penetration member. ### Claim 5 (depends on 1) The device of claim 1, wherein at least part of the device is configured as a handheld, point-of-care device. ### Claim 6 (depends on 1) The device of claim 1, wherein the laser source is configured to deliver a laser beam having a wavelength that excites at least one of hemoglobin and oxyhemoglobin present in the at least one blood vessel. ### Claim 7 (depends on 1) The device of claim 1, further including a data component comprising: a memory storing computer-executable instructions; and a processor to access the memory and execute the computer-executable instructions to at least: record data generated upon detection of the target analyte; analyze the data; and display the data on a graphical user interface. ### Claim 8 A method for collecting a fluid sample from the dermis of a subject, the method comprising the steps of: contacting a device with the skin of a subject so that at least a distal end of one or more penetrating members comprising the device directly contacts the dermis; activating a laser source coupled to the device to deliver a laser beam to the dermis for a time sufficient to induce leakage or rupture of at least one blood vessel comprising the dermis; collecting a volume of a fluid leaked from the at least one blood vessel; and assaying the collected fluid for the presence of one or more target analytes. ### Claim 9 (depends on 8) The method of claim 8, wherein, prior to contacting the device with the skin of a subject, at least a portion of the distal end is coated with one or more capture reagents for capturing one or more target analytes present in the leaked fluid.