# US 10,500,305 B2 · Device and method for a nanofiber wrap to minimize inflamation and scarring

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- Number: US 10,500,305 B2
- Document ID: US10500305B2
- Status: Granted
- Kind: B2
- Application number: 15/128,257
- Filed: 2015-04-09
- Published: 2017-04-06
- Granted: 2019-12-10
- Earliest claimed: 2014-04-10
- Applicants: The Johns Hopkins University
- Inventors: Ibrahim Zuhaib; Hai-Quan Mao; Kellin Krick; Russell Martin; Gerald Brandacher; Karim A. Sarhane
- Web page: https://patentgrep.com/patent/US10500305B2

## CPC

- A61L 27/18: obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
- A61L 27/26: Mixtures of macromolecular compounds
- A61L 27/50: Materials characterised by their function or physical properties {, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials}
- A61L 27/56: Porous materials, {e.g. foams or sponges}
- D01D 5/003: {the material being a polymer solution or dispersion (D01D5/0053 takes precedence)}
- D01F 8/14: with at least one polyester as constituent
- A61L 2400/12: Nanosized materials, e.g. nanofibres, nanoparticles, nanowires, nanotubes; Nanostructured surfaces
- A61L 2430/32: for nerve reconstruction
- D10B 2509/00: Medical; Hygiene
- A61L: METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES (preservation of bodies or disinfecting characterised by the agents employed A01N; preserving, e.g. sterilising, food or foodstuffs A23; preparations for medical, dental or toiletry purposes A61K)
- D01D: MECHANICAL METHODS OR APPARATUS IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS
- D01F: CHEMICAL FEATURES IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS; APPARATUS SPECIALLY ADAPTED FOR THE MANUFACTURE OF CARBON FILAMENTS
- D10B: INDEXING SCHEME ASSOCIATED WITH SUBLASSES OF SECTION D, RELATING TO TEXTILES

## Abstract

The present invention is directed to a device and method for a nanofiber wrap to minimize inflation and scarring of nerve tissue and maximize the nutrient transport. More particularly, the present invention is directed to a novel semi-permeable nanofiber construct prepared from biocompatible materials. The nanofiber construct is applied around a nerve repair site following end-to-end anastomosis. The nanofiber construct is porous and composed of randomly oriented nanofibers prepare using an electrospinning method. The nanofiber construct has a wall that is approximately 50-100 μm thick with pores smaller than 25 μm. The nanofiber construct prevents inflammatory cells from migrating into the nerve coaption site, while still permitting the diffusion of growth factors and essential nutrients. The nanofiber construct allows for enhanced neuroregeneration and optimal function outcomes.

## Claims

### Claim 1

A device for promoting healing at a connection site between tubular biologic structures comprising:
a porous, semipermeable construct of nanofibers;
wherein the nanofibers are spun from a biocompatible material in a sheet configured and sized for wrapping around the connection site tubular biologic structures; and
wherein the sheet is configured to have a pore size to entrap inflammatory cells and prevent infiltration of inflammatory cells to the connection site, while permitting nutrient supply.

### Claim 2 (depends on 1)

The device of claim 1 further comprising the sheet being approximately 50 μm to 500 μm thick.

### Claim 3 (depends on 1)

The device of claim 1 further comprising the sheet being approximately 100 μm thick.

### Claim 4 (depends on 1)

The device of claim 1 further comprising the pore size of approximately 0.5-25 μm.

### Claim 5 (depends on 1)

The device of claim 1 wherein the biocompatible material comprises one selected from a group consisting of polycaprolactone, polylactide, or polyglycolide.

### Claim 6 (depends on 1)

The device of claim 1 wherein the biocompatible material comprises a polymer blend of polycaprolactone and collagen.

### Claim 7 (depends on 1)

The device of claim 1 further comprising each one of the nanofibers having a diameter of approximately 100 nm to 10 μm.

### Claim 8 (depends on 1)

The device of claim 1 wherein the nanofibers are formed by electrospinning method.

### Claim 9 (depends on 8)

The device of claim 8 wherein a needle of the syringe has a gauge of approximately 27, a distance between the source of voltage and a conductive material of approximately 6 cm, an applied voltage from the source of voltage of approximately 7.5 kV, and a flow rate of solution from the syringe of approximately 0.75 mL/h.