Cosmetic compositions from fish hatching fluid

A method of preparing a pharmaceutical or cosmetic composition from fish hatching fluid comprising at least the steps of:

• a) suspending fish eggs in a minimal volume of water;
• b) inducing synchronized, rapid hatching of said eggs;
• c) optionally filtering the hatched eggs to obtain hatching fluid;
• and
• d) filtering the hatching fluid of b) or c) to obtain a composition, wherein the step of filtering the hatching fluid comprises at least the steps of:
• (i) filtering the hatching fluid using a filter with a pore size of at least 5 μm and collecting the filtrate;
• (ii) subjecting the filtrate from step (i) to ion exchange chromatography comprising:
• (1) loading the filtrate on to a DEAE (diethylaminoethyl) ion exchange column;
• (2) washing the column with a buffered wash solution at a pH of 7-9;
• (3) eluting leukolectin polypeptides from the column using a first elution buffer comprising the buffered wash solution further comprising a salt at a concentration of 50-100 mM;
• (4) eluting the remaining polypeptides from the column using a second elution buffer comprising the buffered wash solution comprising a salt at a concentration of 500 mM to 2M;
• (5) collecting the eluate from step (4);
• e) exchanging the water in the eluate from step (5) with a pharmaceutically or cosmetically acceptable buffer by performing diafiltration using a filter with an exclusion size of less than 12 kDa;
• f) filtering the solution obtained from step (e) using a filter with a pore size of 0.15-0.30 μm and collecting the filtrate;
• and
• g) preparing said pharmaceutical or cosmetic composition from the filtrate from step (f).