# US 10,500,242 B2 · Method of producing high-nepodin-containing rumex plant extract and high-nepodin-containing rumex plant extract > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 10,500,242 B2 - Document ID: US10500242B2 - Status: Granted - Kind: B2 - Application number: 16/089,370 - Filed: 2017-02-21 - Published: 2019-04-11 - Granted: 2019-12-10 - Earliest claimed: 2016-03-31 - Assignees: University of the Ryukyus; Okinawa Research Center Co., Ltd. - Applicants: Okinawa Research Center Co., Ltd.; University of the Ryukyus - Inventors: Je-Tae Woo; Yuto Teruya; Kozue Sasaki; Toshiaki Teruya; Aki Yamano; Kosuke Sueyoshi; Miki Yamada - Web page: https://patentgrep.com/patent/US10500242B2 ## CPC - A61K 36/70: Polygonaceae (Buckwheat family), e.g. spineflower or dock - A61K 31/12: Ketones - A61K 2236/39: Complex extraction schemes, e.g. fractionation or repeated extraction steps - A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D) ## Abstract An objective of the present invention is to provide a method for producing a nepodin-containing extract containing a high concentration of nepodin with less contaminants present therein thereby being able to be readily carried out on an industrial scale, when compared to the conventional techniques. The objective is solved by A method for producing a nepodin-containing Rumex plant extract, the method comprising the following steps (1) to (5): (1) a step of subjecting a Rumex plant to an extraction treatment using a nepodin-dissolving solvent to obtain a crude Rumex plant extract; (2) a step of subjecting the crude Rumex plant extract to a water-adding treatment and/or concentration treatment to obtain a solid crude Rumex plant extract; (3) a step of subjecting the solid crude Rumex plant extract to a weak alkali treatment to obtain a weak alkali insoluble product; (4) a step of subjecting the weak alkali insoluble product to a strong alkali treatment to obtain a strong alkali soluble product; and (5) a step of subjecting the strong alkali soluble product to a neutralization treatment to obtain the nepodin-containing Rumex plant extract. ## Claims ### Claim 1 A method for producing a nepodin-containing Rumex plant extract, the method comprising the following steps (1) to (5): (1) a step of subjecting a Rumex plant to an extraction treatment using a nepodin-dissolving solvent to obtain a crude Rumex plant extract; (2) a step of subjecting the crude Rumex plant extract to a water-adding treatment and/or concentration treatment to obtain a solid crude Rumex plant extract; (3) a step of subjecting the solid crude Rumex plant extract to a weak alkali treatment to obtain a weak alkali insoluble product; (4) a step of subjecting the weak alkali insoluble product to a strong alkali treatment to obtain a strong alkali soluble product; and (5) a step of subjecting the strong alkali soluble product to a neutralization treatment to obtain the nepodin-containing Rumex plant extract. ### Claim 2 (depends on 1) The method for producing a nepodin-containing Rumex plant extract according to claim 1, wherein the nepodin-dissolving solvent is 20% to 100% (v/v) ethanol. ### Claim 3 (depends on 1) The method for producing a nepodin-containing Rumex plant extract according to claim 1, wherein the weak alkali treatment is a weak alkali treatment using a weak alkaline aqueous solution having a pH of 8 to 10. ### Claim 4 (depends on 1) The method for producing a nepodin-containing Rumex plant extract according to claim 1, wherein the weak alkali treatment is a weak alkali treatment using a weak alkaline aqueous solution obtained by mixing sodium bicarbonate and water, wherein the solution has a pH of 8 to 10. ### Claim 5 (depends on 1) The method for producing a nepodin-containing Rumex plant extract according to claim 1, wherein the strong alkali treatment is a strong alkali treatment using a strong alkaline aqueous solution having a pH of 12 to 14. ### Claim 6 (depends on 1) The method for producing a nepodin-containing Rumex plant extract according to claim 1, wherein the strong alkali treatment is a strong alkali treatment using a strong alkaline aqueous solution obtained by mixing sodium hydroxide and water, wherein the solution has a pH of 12 to 14. ### Claim 7 (depends on 1) The method for producing a nepodin-containing Rumex plant extract according to claim 1, wherein the neutralizing treatment is a neutralizing treatment using an acidic aqueous solution having a pH of 1 to 3. ### Claim 8 (depends on 1) The method for producing a nepodin-containing Rumex plant extract according to claim 1, wherein the neutralizing treatment is a neutralizing treatment using an acidic aqueous solution obtained by mixing water and an acid component selected from the group consisting of acetic acid and hydrochloric acid, wherein the solution has a pH of 1 to 3. ### Claim 9 (depends on 1) The method for producing a nepodin-containing Rumex plant extract according to claim 1, wherein the nepodin-containing Rumex plant extract is a nepodin-containing Rumex plant extract containing 20 wt % to 40 wt % of nepodin. ### Claim 10 (depends on 1) A nepodin-containing Rumex plant extract containing 20 wt % to 40 wt % nepodin and 60 wt % or less fatty acids obtained by the method according to claim 1. ### Claim 11 (depends on 10) The nepodin-containing Rumex plant extract according to claim 10, wherein the nepodin-dissolving solvent is 20% to 100% (v/v) ethanol. ### Claim 12 (depends on 10) The nepodin-containing Rumex plant extract according to claim 10, wherein the weak alkali treatment is a weak alkali treatment using a weak alkaline aqueous solution having a pH of 8 to 10. ### Claim 13 (depends on 10) The nepodin-containing Rumex plant extract according to claim 10, wherein the weak alkali treatment is a weak alkali treatment using a weak alkaline aqueous solution obtained by mixing sodium bicarbonate and water, wherein the solution has a pH of 8 to 10. ### Claim 14 (depends on 10) The nepodin-containing Rumex plant extract according to claim 10, wherein the strong alkali treatment is a strong alkali treatment using a strong alkaline aqueous solution having a pH of 12 to 14. ### Claim 15 (depends on 10) The nepodin-containing Rumex plant extract according to claim 10, wherein the strong alkali treatment is a strong alkali treatment using a strong alkaline aqueous solution obtained by mixing sodium hydroxide and water, wherein the solution has a pH of 12 to 14. ### Claim 16 (depends on 10) The nepodin-containing Rumex plant extract according to claim 10, wherein the neutralizing treatment is a neutralizing treatment using an acidic aqueous solution having a pH of 1 to 3. ### Claim 17 (depends on 10) The nepodin-containing Rumex plant extract according to claim 10, wherein the neutralizing treatment is a neutralizing treatment using an acidic aqueous solution obtained by mixing water and an acid component selected from the group consisting of acetic acid and hydrochloric acid, wherein the solution has a pH of 1 to 3. ### Claim 18 (depends on 10) The nepodin-containing Rumex plant extract according to claim 10, wherein the nepodin-containing Rumex plant extract is a nepodin-containing Rumex plant extract containing 20 wt % to 40 wt % of nepodin.