# US 10,500,222 B2 · Compositions and methods relating to treatment of infection

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- Number: US 10,500,222 B2
- Document ID: US10500222B2
- Status: Granted
- Kind: B2
- Application number: 15/297,438
- Filed: 2016-10-19
- Published: 2017-04-20
- Granted: 2019-12-10
- Earliest claimed: 2015-10-19
- Assignees: Wayne State University
- Applicants: Wayne State University
- Inventors: Linda D. Hazlett
- Web page: https://patentgrep.com/patent/US10500222B2

## CPC

- A61K 31/704: attached to a condensed carbocyclic ring system, e.g. sennosides, thiocolchicosides, escin, daunorubicin {(digitoxin A61K31/7048)}
- A61K 9/0048: {Eye, e.g. artificial tears}
- A61K 45/06: Mixtures of active ingredients without chemical characterisation, e.g. antiphlogistics and cardiaca
- A61K 9/0051: {Ocular inserts or implants}
- A61K 9/08: Solutions {(composition of solutions A61K47/00)}
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)

## Abstract

Microbial keratitis is a sight threatening disorder and infections result in ocular pain, stromal destruction, corneal thinning and/or perforation, leading to vision loss, if untreated and the need for transplantation. Methods of treating bacterial infection of the cornea prophylactically or therapeutically in a subject, are provided according to aspects of the present invention which include administering an effective dose of glycyrrhizin, or a pharmaceutically acceptable salt, hydrate, solvate, ester, amide, isomer or derivative thereof characterized by anti-bacterial activity, to the subject.

## Claims

### Claim 1

A method of treating Pseudomonas aeruginosa infection of the cornea therapeutically in a subject, comprising:
administering a composition comprising an effective dose of glycyrrhizin, or a pharmaceutically acceptable salt, hydrate, solvate, amide, or isomer thereof to the subject, wherein no additional therapeutic agent is included in the composition, thereby reducing bacterial load in the cornea and thereby treating Pseudomonas aeruginosa infection of the cornea therapeutically in the subject.

### Claim 2 (depends on 1)

The method of claim 1, wherein administering the effective dose of glycyrrhizin, or a pharmaceutically acceptable salt, hydrate, solvate, amide, or isomer thereof, comprises subconjunctival administration.

### Claim 3 (depends on 1)

The method of claim 1, wherein administering the effective dose of glycyrrhizin, or a pharmaceutically acceptable salt, hydrate, solvate, amide, or isomer thereof, comprises topical administration to the cornea.

### Claim 4 (depends on 1)

The method of claim 1, wherein administering the effective dose of glycyrrhizin, or a pharmaceutically acceptable salt, hydrate, solvate, amide, or isomer thereof, comprises systemic administration.

### Claim 5 (depends on 1)

The method of claim 1, wherein administering the effective dose of glycyrrhizin, or a pharmaceutically acceptable salt, hydrate, solvate, amide, or isomer thereof, comprises parenteral administration.

### Claim 6 (depends on 1)

The method of claim 1, wherein administering the effective dose of glycyrrhizin, or a pharmaceutically acceptable salt, hydrate, solvate, amide, or isomer thereof, comprises intravenous administration.

### Claim 7 (depends on 1)

The method of claim 1, wherein the effective dose of glycyrrhizin, or a pharmaceutically acceptable salt, hydrate, solvate, amide, or isomer thereof, is administered to the subject within 24 hours of a traumatic ocular surface event wherein the traumatic ocular surface event caused a Pseudomonas aeruginosa infection of the cornea.

### Claim 8 (depends on 1)

The method of claim 1, wherein the subject is human.

### Claim 9 (depends on 1)

The method of claim 1, wherein the subject is a horse.