# US 10,500,153 B2 · Exopolysaccharide for the treatment and/or care of the skin, culture media and compositions

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- Number: US 10,500,153 B2
- Document ID: US10500153B2
- Status: Granted
- Kind: B2
- Application number: 15/032,840
- Filed: 2014-10-30
- Published: 2016-09-15
- Granted: 2019-12-10
- Earliest claimed: 2013-10-30
- Assignees: Lipotec S.A.U.
- Applicants: Lipotec, S.A.U.
- Inventors: Núria García Sanz; Antonio Vicente Ferrer Montiel; Albert Soley Astals; Nuria Almiñana Doménech
- Web page: https://patentgrep.com/patent/US10500153B2

## CPC

- A61K 8/99: from microorganisms other than algae or fungi, e.g. protozoa or bacteria
- A61K 8/0216: {Solid or semisolid forms}
- A61K 8/042: {Gels}
- A61K 8/068: {Microemulsions}
- A61K 8/14: Liposomes; Vesicles
- A61K 8/34: Alcohols
- A61K 8/342: {Alcohols having more than seven atoms in an unbroken chain}
- A61K 8/375: {the alcohol moiety containing more than one hydroxy group}
- A61K 8/55: Phosphorus compounds
- A61K 8/73: Polysaccharides
- A61K 8/86: Polyethers
- A61K 8/891: saturated, e.g. dimethicone, phenyl trimethicone, C24-C28 methicone or stearyl dimethicone
- A61P 17/00: Drugs for dermatological disorders
- A61P 17/02: for treating wounds, ulcers, burns, scars, keloids, or the like
- A61P 29/00: Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
- A61Q 19/06: for countering cellulitis
- A61Q 19/08: Anti-ageing preparations
- C12P 19/04: Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
- A61K 2800/5426: cationic
- A61K 2800/596: Mixtures of surface active compounds
- A61K 2800/75: Anti-irritant
- A61K 2800/78: Enzyme modulators, e.g. Enzyme agonists
- A61K 2800/85: Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61Q: SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- C12P: FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {(brewing of beer C12C; producing vinegar C12J; producing specific peptides or proteins C07K; producing enzymes C12N9/00; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification C12N15/00; measuring or testing processes involving enzymes or microorganisms C12Q; measuring or testing processes involving nucleic acid amplification reactions C12Q1/6844; fermentation processes to form a food composition, A21 or A23; compounds in general, see the relevant compound class, e.g. C01, C07)}

## Abstract

Exopolysaccharide of a bacterial strain for its use in treatment and/or care of the skin, as well as its cosmetic and/or dermopharmaceutical compositions. In particular, its use for inflammation, lipolysis, lipid accumulation and skin firmness.

## Claims

### Claim 1

A cosmetic composition for topical or transdermal application comprising a cosmetically effective quantity of the exopolysaccharide produced by a strain of Halomonas anticariensis species with deposit number LMG P-27891 and at least one cosmetically acceptable excipient and/or ingredient, wherein the exopolysaccharide has a composition by weight of 1% to 22% of glucose, 50% to 85% of mannose, 15% to 30% of rhamnose, up to 3% by weight galacturonic acid and up to 4%, by weight xylose, with the condition that the sum of the percentages does not exceed 100%, and wherein the composition:
is in a formulation selected from the group consisting of creams, multiple emulsions, liquid crystals, anhydrous compositions, foams, gels, cream gels, hydroalcoholic solutions, hydroglycolic solutions, hydrogels, liniments, soaps, shampoos, conditioners, polysaccharide films, ointments, mousses, powders, bars, pencils, sprays, and aerosols; and/or
is incorporated into a cosmetic delivery system and/or sustained release system or is adsorbed on a solid organic polymer or solid mineral support: and/or
comprises liposomes, lipid nanoparticles, surfactant-phospholipid mixed micelles, or water-in-oil microemulsions containing the exopolysaccharide; and/or
is incorporated into fabric, non-woven fabric or a medical device, wherein the medical device is selected from the group consisting of bandages, gauzes, dressings, adhesive patches, non-adhesive patches, occlusive patches, microelectric patches and face masks.

### Claim 2 (depends on 1)

The cosmetic composition according to claim 1, wherein the at least one cosmetically acceptable excipient and/or ingredient is selected from the group consisting of agents that reduce the amount of nocturnin, agents inhibiting the nocturnin expression, lipolytic agents or agents stimulating lipolysis, venotonic agents, agents modulating PGC-1α expression, agents inhibiting the activity of PPARγ, agents which reduce the triglyceride content of adipocytes, anti-cellulite agents, agents delaying adipocyte differentiation, agents which diminish the sebum production, agents stimulating the synthesis of dermal or epidermal macromolecules and/or capable of inhibiting or preventing their degradation, collagen synthesis-stimulating agents, elastin synthesis-stimulating agents, decorin synthesis-stimulating agents, laminin synthesis-stimulating agents, defensin synthesis-stimulating agents, chaperone synthesis-stimulating agents, cAMP synthesis-stimulating agents, agents that modulate AQP-3, agents that modulate aquaporin synthesis, proteins from the aquaporin family, hyaluronic acid synthesis-stimulating agents, glycosaminoglycan synthesis-stimulating agents, fibronectin synthesis-stimulating agents, sirtuin synthesis-stimulating agents, heat shock proteins, heat shock protein synthesis-stimulating agents, agents which inhibit neuronal exocytosis, anticholinergic agents, agents which inhibit muscular contraction, anti-aging agents, anti-wrinkle agents, antiperspirant agents, anti-inflammatory agents and/or analgesics, anti-itching agents, calming agents, anesthetic agents, inhibitors of acetylcholine-receptor aggregation, agents that inhibit acetylcholinesterase, skin relaxant agents, melanin synthesis stimulating or inhibiting agents, whitening or depigmenting agents, propigmenting agents, self-tanning agents, NO-synthase inhibiting agents, 5α-reductase inhibiting agents, lysyl- and/or prolyl hydroxylase inhibiting agents, antioxidants, free radical scavengers and/or agents against atmospheric pollution, reactive carbonyl species scavengers, anti-glycation agents, antihistamine agents, antiviral agents, antiparasitic agents, emulsifiers, emollients, organic solvents, liquid propellants, skin conditioners, humectants, substances that retain moisture, alpha hydroxy acids, beta hydroxy acids, moisturizers, epidermal hydrolytic enzymes, vitamins, amino acids, proteins, pigments or colorants, dyes, biopolymers, gelling polymers, thickeners, surfactants, softening agents, emulsifiers, binding agents, preservatives, agents able to reduce or treat the bags under the eyes, exfoliating agents, keratolytic agents, desquamating agents, antimicrobial agents, antifungal agents, fungistatic agents, bactericidal agents, bacteriostatic agents, agents stimulating the synthesis of components of the stratum corneum, ceramides, fatty acids, agents that inhibit collagen degradation, agents that inhibit matrix metalloproteinases, agents that inhibit elastin degradation, agents that inhibit serine proteases, agents stimulating fibroblast proliferation, agents stimulating keratinocyte proliferation, agents stimulating melanocyte proliferation, agents stimulating keratinocyte differentiation, antihyperkeratosis agents, comedolytic agents, anti-psoriasis agents, DNA repair agents, DNA protecting agents, stem cell protecting agents, stabilizers, agents for the treatment and/or care of sensitive skin, firming agents, anti-stretch mark agents, astringent agents, agents which inhibit the activity of PAR-2, agents stimulating healing, coadjuvant healing agents, agents stimulating reepithelialization, coadjuvant reepithelialization agents, cytokine growth factors, agents acting on capillary circulation and/or microcirculation, agents stimulating angiogenesis, agents that inhibit vascular permeability, agents acting on cell metabolism, agents to improve dermal-epidermal junction, agents inducing hair growth, hair growth inhibiting or retardant agents, hair loss retardant agents, preservatives, perfumes, cosmetic and/or absorbent and/or body odor masking deodorants, chelating agents, plant extracts, essential oils, marine extracts, agents obtained from a biotechnological process, mineral salts, cell extracts, sunscreens and organic or mineral photoprotective agents active against ultraviolet A and/or B rays and/or infrared A rays, or mixtures thereof.

### Claim 3 (depends on 1)

The cosmetic composition according to claim 1, wherein the exopolysaccharide excreted by the strain of Halomonas anticariensis species has a residence time between 4 and 10 minutes at a HPLC analysis with a chromatographic column for aqueous Size Exclusion Chromatography, with a particle size of 8 μm, a pore size of 50 Å and a length/Internal Diameter of 300 mm×7.5 mm, water with sodium acetate 0.1 M as eluent and flow rate 0.8 ml/min.

### Claim 4 (depends on 1)

A method for cosmetic, non-therapeutic treatment and/or care of the skin comprising applying the cosmetic composition of claim 1 to the skin.

### Claim 5 (depends on 4)

The method of claim 4, wherein the cosmetic, non-therapeutic treatment and/or care comprises at least one of: treatment of cellulite, treatment of reduction of lipid accumulation in the skin, stimulation of lipolysis in the skin, stimulation of collagen synthesis, treatment of skin aging, treatment of skin wrinkles, treatment for improving skin firmness, prevention of loss of skin firmness, and treatment for improving skin elasticity.

### Claim 6 (depends on 4)

The method of claim 4, wherein the cosmetic, non-therapeutic treatment and/or care reduces the amount of nocturnin in skin cells.

### Claim 7 (depends on 1)

A method for cosmetic, non-therapeutic treatment and/or care of the skin comprising applying a cosmetic composition of claim 1 to the skin, the cosmetic composition comprising liposomes, lipid nanoparticles, surfactant-phospholipid mixed micelles, or water-in-oil microemulsions containing the exopolysaccharide produced by the strain of Halomonas anticariensis species.

### Claim 8 (depends on 1)

A method for treatment of skin wounds comprising applying the cosmetic composition of claim 1 to a skin wound.

### Claim 9 (depends on 1)

The cosmetic composition of claim 1, wherein the at least one cosmetically acceptable excipient and/or ingredient comprises:
between 0.1 weight % and 20 weight % of an humectant selected from the group consisting of glycerin, propylene glycol, butylene glycol, pentylene glycol, caprylyl glycol, lactic acid, urea, and sodium hyaluronate;
between 0.1 weight % and 20 weight % of an emollient or skin conditioning agent selected from the group consisting of dimethicone, glyceryl stearate, caprylic/capric triglyceride, cetearyl alcohol, lecithin, C12-15 alkyl benzoate, squalane, lanolin, behenyl alcohol, tocopheryl acetate, panthenol, Butyrospermum parkii butter, retinyl palmitate, and retinol; and
between 0.1 weight % and 20 weight % of a surfactant selected from the group consisting of xanthan gum, sodium laureth sulfate, stearic acid, polysorbate 20, polysorbate 80, stearyl alcohol, cetyl alcohol, steareth-2, ceteareth-20, and cocamidopropyl betaine.

### Claim 10 (depends on 1)

The cosmetic composition of claim 1, wherein the composition is in a cream formulation.

### Claim 11 (depends on 1)

The cosmetic composition of claim 1, wherein the composition is in a gel or hydrogel formulation.

### Claim 12 (depends on 1)

The cosmetic composition of claim 1, wherein the composition is in a cream gel formulation.

### Claim 13 (depends on 1)

The cosmetic composition of claim 1, wherein the composition is in an anhydrous composition.

### Claim 14 (depends on 1)

The cosmetic composition of claim 1, wherein the composition is in a hydroalcoholic solution.

### Claim 15 (depends on 1)

The cosmetic composition of claim 1, wherein the composition is in a hydroglycolic solution.

### Claim 16 (depends on 1)

The cosmetic composition of claim 1, wherein the composition is in a soap, shampoo, or conditioner.

### Claim 17 (depends on 1)

The cosmetic composition of claim 1, wherein the composition is in a polysaccharide film.

### Claim 18 (depends on 1)

The cosmetic composition of claim 1, wherein the composition is in an ointment, mousse, powder, bar, or pencil formulation.

### Claim 19 (depends on 1)

The cosmetic composition of claim 1, wherein the composition is in a spray formulation.

### Claim 20 (depends on 1)

The cosmetic composition of claim 1, wherein the composition is in an aerosol formulation.

### Claim 21 (depends on 1)

The cosmetic composition of claim 1, wherein the composition is adsorbed on a solid organic polymer or solid mineral support.

### Claim 22 (depends on 1)

The cosmetic composition of claim 1, wherein the composition comprises liposomes containing the exopolysaccharide.

### Claim 23 (depends on 1)

The cosmetic composition of claim 1, wherein the composition comprises lipid nanoparticles containing the exopolysaccharide.

### Claim 24 (depends on 1)

The cosmetic composition of claim 1, wherein the composition comprises surfactant-phospholipid mixed micelles containing the exopolysaccharide.

### Claim 25 (depends on 1)

The cosmetic composition of claim 1, wherein the composition comprises a water-in-oil microemulsion containing the exopolysaccharide.

### Claim 26 (depends on 1)

The cosmetic composition of claim 1, wherein the composition is incorporated into fabric, non-woven fabric or medical device, wherein the medical device is selected from the group consisting of bandages, gauzes, dressings, adhesive patches, non-adhesive patches, occlusive patches, microelectric patches and face masks.