# US 10,500,051 B2 · Pouch-like construct for preventing heart distension > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 10,500,051 B2 - Document ID: US10500051B2 - Status: Granted - Kind: B2 - Application number: 15/272,022 - Filed: 2016-09-21 - Published: 2017-03-16 - Granted: 2019-12-10 - Earliest claimed: 2006-11-11 - Assignees: Universitätsklinikum Hamburg-Eppendorf - Applicants: Universitatsklinikum Hamburg-Eppendorf - Inventors: Wolfram-Hubertus Zimmermann; Thomas Eschenhagen; Yalin Yildirim - Web page: https://patentgrep.com/patent/US10500051B2 ## CPC - A61F 2/2481: {Devices outside the heart wall, e.g. bags, strips or bands} - A61K 35/34: Muscles; Smooth muscle cells; Heart; Cardiac stem cells; Myoblasts; Myocytes; Cardiomyocytes (vascular smooth muscle A61K35/44) - A61L 27/38: {containing added animal cells (organs or tissue containing native cells A61L27/36)} - A61L 27/54: Biologically active materials, e.g. therapeutic substances {(A61L27/227 takes precedence)} - A61F 2240/001: Designing or manufacturing processes - A61L 2300/412: Tissue-regenerating or healing or proliferative agents - A61L 2300/414: Growth factors - A61F: FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS (dental prosthetics A61C) - A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D) - A61L: METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES (preservation of bodies or disinfecting characterised by the agents employed A01N; preserving, e.g. sterilising, food or foodstuffs A23; preparations for medical, dental or toiletry purposes A61K) ## Abstract The invention is directed to a pouch-like construct comprising mammalian tissue which can be used for preventing distension and/or resisting dilation of the heart in a mammal. Preferably, the pouch-like tissue construct has contractile properties. The invention further relates to a method for the preparation of a pouch-like construct comprising mammalian tissue which can be used for the above purposes. ## Claims ### Claim 1 A method for the preparation of a pouch-like construct suitable for enclosing at least part of a heart of a mammal and comprising engineered tissue, said engineered tissue comprising cardiac myocytes, wherein the method comprises the steps of: a) providing a matrix having a recess in one surface of the matrix, wherein said recess has desired dimensions corresponding to desired dimensions of an exterior of the pouch-like construct for the pouch-like construct's exterior to be formed, b) providing a body having dimensions corresponding to desired dimensions of an interior of the pouch-like construct for the pouch-like construct's interior to be formed, c) disposing the body in the recess of the matrix such that the body is spaced from walls of the recess to form a space between the body and the walls of the recess corresponding to the desired dimensions of the construct, d) disposing a reconstitution mixture in the recess, wherein said reconstitution mixture comprises mammalian cells and suitable scaffold material which can be incubated to form an engineered tissue construct, said construct comprising cardiac myocytes, e) contacting the reconstitution mixture disposed within the recess with a culture medium suitable for culturing the cells in the reconstitution mixture, f) incubating the reconstitution mixture until a tissue construct forms within the matrix recess between the body and the matrix, g) removing the body with the tissue construct adherent thereto from the matrix recess, and h) separating the tissue construct from the body. ### Claim 2 (depends on 1) The method according to claim 1, wherein the step of providing a matrix includes providing a porous matrix filled with a liquid in order to impede outflow of the reconstitution mixture from the recess into the porous matrix, and wherein the step of contacting the reconstitution mixture disposed within the recess with a culture medium includes disposing a liquid culture medium on the surface of the matrix in which the recess is formed in order to form a layer of the culture medium on top of the matrix and the reconstitution mixture disposed within the recess. ### Claim 3 (depends on 1) The method according to claim 1, wherein the step of providing a matrix includes providing a porous matrix including the culture medium. ### Claim 4 (depends on 1) The method according to claim 1, wherein the body is spherical. ### Claim 5 (depends on 1) The method according to claim 1, wherein the body comprises a surface of glass, polytetrafluoroethylene (PTFE), or silicone. ### Claim 6 (depends on 1) The method according to claim 1, wherein the body is an inflatable structure. ### Claim 7 (depends on 1) The method according to claim 1, wherein step c) is performed prior to step d). ### Claim 8 (depends on 1) The method according to claim 1, wherein step d) is performed prior to step c). ### Claim 9 (depends on 1) The method according to claim 1, wherein after step h), the construct is subjected to tensile stress. ### Claim 10 (depends on 9) The method according to claim 9, wherein the tensile stress is static, phasic or auxotonic stress or a combination thereof. ### Claim 11 (depends on 10) The method according to claim 10, wherein the tensile stress is auxotonic stress.