# US 10,499,676 B2 · Intestinal barrier integrity > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 10,499,676 B2 - Document ID: US10499676B2 - Status: Granted - Kind: B2 - Application number: 14/739,850 - Filed: 2015-06-15 - Published: 2016-03-17 - Granted: 2019-12-10 - Earliest claimed: 2012-08-24 - Assignees: N.V. Nutricia - Applicants: N.V. Nutricia - Inventors: Eric Alexander Franciscus Van Tol; Linette Eustachia Maria Willemsen; Marleen Antoinette Koetsier; Christopher Beermann; Bernd Stahl - Web page: https://patentgrep.com/patent/US10499676B2 ## CPC - A23L 29/04: {Fatty acids or derivatives} - A23L 29/30: containing carbohydrate syrups; containing sugars; containing sugar alcohols, e.g. xylitol; containing starch hydrolysates, e.g. dextrin (products from apiculture A23L21/20; artificial sweetening agents A23L27/30) - A23L 33/12: Fatty acids or derivatives thereof - A23L 33/40: {Complete food formulations for specific consumer groups or specific purposes, e.g. infant formula} - A61K 31/202: having three or more double bonds, e.g. linolenic (eicosanoids, e.g. leukotrienes A61K31/557) - A61K 31/702: Oligosaccharides, i.e. having three to five saccharide radicals attached to each other by glycosidic linkages - A61P 1/00: Drugs for disorders of the alimentary tract or the digestive system - A61P 1/12: Antidiarrhoeals - A61P 37/08: Antiallergic agents (antiasthmatic agents A61P11/06; ophthalmic antiallergics A61P27/14) - A23V 2002/00: Food compositions, function of food ingredients or processes for food or foodstuffs - A23L: FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF (preservation thereof A23B) - A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D) - A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS - A23V: INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION ## Abstract The invention concerns a method for stimulating barrier integrity in a mammal by administering to a mammal a composition comprising: eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA) and arachidonic acid (ARA), and at least two distinct oligosaccharides. ## Claims ### Claim 1 A nutritional composition comprising: (a) eicosapentaenoic acid (EPA) and arachidonic acid (ARA), wherein the content of long chain polyunsaturated fatty acid with 20 and 22 carbon atoms does not exceed 15 wt. % of the total fat content; and (b) at least two distinct oligosaccharides with a degree of polymerisation (DP) between 2 and 60 comprising transgalactooligosaccharide (TOS) and inulin. ### Claim 2 (depends on 1) The composition according to claim 1, wherein at least 10 wt. % of the oligosaccharides has a degree of polymerisation (DP) between 2 to 5 and at least 5 wt. % of the oligosaccharides has a DP between 10 and 60. ### Claim 3 (depends on 1) The composition according to claim 1, further comprising an acidic oligosaccharide with a DP between 2 and 60. ### Claim 4 (depends on 1) The composition according to claim 1, further comprising DHA. ### Claim 5 (depends on 2) The composition according to claim 2, further comprising an acidic oligosaccharide with a DP between 2 and 60. ### Claim 6 (depends on 3) The composition according to claim 3, wherein the acidic oligosaccharide is an uronic acid polymer with a DP between 2 and 6. ### Claim 7 (depends on 1) The composition according to claim 1, comprising 7.5 to 12.5 energy % protein; 40 to 55 energy % carbohydrates; and 35 to 50 energy % fat, wherein said protein comprises hydrolyzed milk protein, vegetable protein, amino acids, or combinations thereof. ### Claim 8 (depends on 2) The composition according to claim 2, comprising 7.5 to 12.5 energy % protein; 40 to 55 energy % carbohydrates; and 35 to 50 energy % fat, wherein said protein comprises hydrolyzed milk protein, vegetable protein, amino acids, or combinations thereof. ### Claim 9 (depends on 3) The composition according to claim 3, comprising 7.5 to 12.5 energy % protein; 40 to 55 energy % carbohydrates; and 35 to 50 energy % fat, wherein said protein comprises hydrolyzed milk protein, vegetable protein, amino acids, or combinations thereof. ### Claim 10 (depends on 1) The composition according to claim 1, comprising 7.5 to 12.5 energy % protein; 40 to 55 energy % carbohydrates; and 35 to 50 energy % fat, wherein said protein comprises hydrolyzed milk protein, vegetable protein, amino acids, or combinations thereof. ### Claim 11 (depends on 1) The composition according to claim 1, having a caloric content of 0.6 to 0.8 kcal/ml; an osmolality of 50 to 500 mOsm/kg; and a viscosity below 50 mPas. ### Claim 12 (depends on 2) The composition according to claim 2, having a caloric content of 0.6 to 0.8 kcal/ml; an osmolality of 50 to 500 mOsm/kg; and a viscosity below 50 mPas. ### Claim 13 (depends on 3) The composition according to claim 3, having a caloric content of 0.6 to 0.8 kcal/ml; an osmolality of 50 to 500 mOsm/kg; and a viscosity below 50 mPas. ### Claim 14 (depends on 1) The composition according to claim 1, having a caloric content of 0.6 to 0.8 kcal/ml; an osmolality of 50 to 500 mOsm/kg; and a viscosity below 50 mPas. ### Claim 15 (depends on 7) The composition according to claim 7, having a caloric content of 0.6 to 0.8 kcal/ml; an osmolality of 50 to 500 mOsm/kg; and a viscosity below 50 mPas. ### Claim 16 (depends on 1) The composition according to claim 1, wherein: (a) the content of long chain polyunsaturated fatty acid is below 3 wt. % of the total fat content; (b) the content of omega-3 long chain polyunsaturated fatty acid is below 1 wt. % of the total fat content; and (c) the content of omega-6 long chain polyunsaturated fatty acid is below 2 wt. % of the total fat content. ### Claim 17 (depends on 1) The composition according to claim 1, further comprising between 5 and 100 mg nucleosides and/or nucleotides per 100 gram dry formula. ### Claim 18 (depends on 1) The composition according to claim 1, which is an infant formula.