Methods and compositions for treatment and control of plant disease
AssigneeThe Texas A&M University System
InventorsCarlos F. Gonzalez, Stephen J. Ahern, Mayukh Das, Ryland F. Young, III, Tushar Suvra Bhowmick
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Abstract
The present invention provides methods for development of a virulent bacteriophage-based treatment for the control of plant diseases caused by Xylella fastidiosa. The invention further provides methods of isolating and propagating bacteriophage virulent to X. fastidiosa in a Xanthomonas bacterial host and for treating or reducing symptoms of X. fastidiosa infection in a plant. The invention further provides methods of isolating and propagating bacteriophage virulent to Xanthomonas axonopodis pv. citri and for treating or reducing symptoms of Xanthomonas axonopodis pv. citri infection in a plant.
Claims5
A plant disease biocontrol composition formulated for delivery to a plant, the composition comprising at least one carrier comprising a pH buffering agent, and at least two bacteriophage isolates of Xfas100 phage type or Xfas300 phage type, or both;
- wherein each of said bacteriophage isolates is virulent to Xylella fastidiosa and Xanthomonas species, and the bacteriophages are capable of lysing said Xylella fastidiosa or Xanthomonas bacteria, further wherein
- the Xfas100 phage type displays the following characteristics:
- (a) the bacteriophage infects a cell by binding to a Type IV pilus;
- (b) the bacteriophage comprises a non-contractile tail and a capsid size ranging from 55-77 nm in diameter and belongs to the Siphoviridae family;
- (c) the genomic size of the bacteriophage is about 55500 bp to 56200 bp;
- and
- (d) the bacteriophage prevents or reduces symptoms associated with Pierce's Disease in a plant or plants;
- and further wherein the Xfas300 phage type displays the following characteristics:
- (e) the bacteriophage infects a cell by binding to a Type IV pilus;
- (f) the bacteriophage comprises a non-contractile tail and a capsid size ranging from 58-68 nm in diameter and belongs to the Podoviridae family;
- (g) the genomic size of the bacteriophage is about 43300 bp to 44600 bp;
- and
- (h) the bacteriophage prevents or reduces symptoms associated with Pierce's Disease in a plant or plants.
Dependencies are inferred from the claim text.