# US 10,499,648 B2 · Sterilization method, formulation for sterilization use, and device for producing sterilizing liquid

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- Number: US 10,499,648 B2
- Document ID: US10499648B2
- Status: Granted
- Kind: B2
- Application number: 15/446,155
- Filed: 2017-03-01
- Published: 2017-06-22
- Granted: 2019-12-10
- Earliest claimed: 2014-09-02
- Applicants: Katsuhisa Kitano; Atsushi Tani; Satoshi Ikawa; Yoichi Nakashima
- Inventors: Katsuhisa Kitano; Atsushi Tani; Satoshi Ikawa; Yoichi Nakashima
- Web page: https://patentgrep.com/patent/US10499648B2

## CPC

- A01N 59/00: Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A61K 33/40: Peroxides
- A61L 2/18: Liquid substances
- A61P 31/02: Local antiseptics
- A61P 31/04: Antibacterial agents
- A61P 31/12: Antivirals
- A61L 2202/11: Apparatus for generating biocidal substances, e.g. vaporisers, UV lamps
- A01N: PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF (preservation of food or foodstuff A23); BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES (preparations for medical, dental or toiletry purposes which kill or prevent the growth or proliferation of unwanted organisms A61K); PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- A61L: METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES (preservation of bodies or disinfecting characterised by the agents employed A01N; preserving, e.g. sterilising, food or foodstuffs A23; preparations for medical, dental or toiletry purposes A61K)
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS

## Abstract

A sterilization method is provided which acquires microbicidal activity equivalent to plasma-treated solution or more powerful than the same without the use of a plasma generation device. The sterilization method includes applying liquid containing peroxynitric acid (HOONO2) produced by chemical reaction to an object to be sterilized under acidic conditions of a pH value of 4.8 or lower. The liquid containing the peroxynitric acid is produced by mixing nitrous acid and peroxide together, for example, mixing nitrite and peroxide together.

## Claims

### Claim 1

A sterilization method comprising:
synthesizing peroxynitric acid (HOONO2) by chemical reaction under acidic conditions of a pH value of 2 or lower to preserve a liquid containing the peroxynitric acid (HOONO2); and
diluting the preserved liquid containing the peroxynitric acid (HOONO2) with a buffer solution, and applying the resultant liquid to an object to be sterilized under acidic conditions of a pH value of 4.8 or lower, thereby to increase the concentration of hydroperoxy radicals (HOO.) produced in the preserved liquid containing the peroxynitric acid (HOONO2); and
sterilizing the object to be sterilized by microbicidal activity of the hydroperoxy radicals (HOO.).

### Claim 2 (depends on 1)

The sterilization method according to claim 1, wherein the preserved liquid containing the peroxynitric acid is produced by mixing a nitrite and peroxide together.

### Claim 3 (depends on 1)

The sterilization method according to claim 1, wherein the preserved liquid containing the peroxynitric acid is produced by dissolving nitrous acid gas into an aqueous solution so as to be mixed with a peroxide.

### Claim 4 (depends on 1)

The sterilization method according to claim 1, comprising
synthesizing, by mixing a nitrite, peroxide, and acid together to produce a liquid having a pH value of 2 or lower, to produce the preserved liquid containing the peroxynitric acid,
using a buffer solution to dilute the preserved liquid containing the peroxynitric acid to produce a liquid having a pH value of 3 to 4.8, and
applying the liquid having a pH value of 3 to 4.8 to the object to be sterilized.

### Claim 5 (depends on 4)

The sterilization method according to claim 4, wherein
the acid is mixed with the peroxide to produce a liquid having a pH value of 2 or lower, and
the liquid having a pH value of 2 or lower is mixed with the nitrite to synthesize the peroxynitric acid.

### Claim 6 (depends on 4)

The sterilization method according to claim 4, wherein
the liquid is adjusted to have a temperature of 10° C. or lower when the peroxynitric acid is synthesized,
thereafter, the temperature of the liquid is increased, and
the liquid is adjusted to have a temperature of 20° C. or higher when the liquid is applied to the object to be sterilized.

### Claim 7 (depends on 2)

The sterilization method according to claim 2, wherein the nitrite is sodium nitrite (NaNO2) and the peroxide is hydrogen peroxide (H2O2).

### Claim 8 (depends on 4)

The sterilization method according to claim 4, wherein the acid is nitric acid (HNO3).