# US 10,499,606 B2 · Petunia-calibrachoa variety SAKPXC023 > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 10,499,606 B2 - Document ID: US10499606B2 - Status: Granted - Kind: B2 - Application number: 16/357,660 - Filed: 2019-03-19 - Published: 2019-07-11 - Granted: 2019-12-10 - Earliest claimed: 2019-03-19 - Assignees: Sakata Seed Corporation - Applicants: Sakata Seed Corporation - Inventors: Jun Tsukahara - Web page: https://patentgrep.com/patent/US10499606B2 ## CPC - A01H 6/824: {Petunia} - A01H 5/02: Flowers - A01H: NEW PLANTS OR {NON-TRANSGENIC} PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES ## Abstract A petunia-calibrachoa plant designated SAKPXC023 is disclosed. Embodiments include the seeds of petunia-calibrachoa SAKPXC023, the plants of petunia-calibrachoa SAKPXC023, to plant parts of petunia-calibrachoa SAKPXC023, and methods for producing a plant produced by crossing petunia-calibrachoa SAKPXC023 with itself or with another variety. Embodiments include methods for producing a plant containing in its genetic material one or more genes or transgenes and the transgenic plants and plant parts produced by those methods. Embodiments also relate to varieties, breeding varieties, plant parts, and cells derived from petunia-calibrachoa SAKPXC023, methods for producing other lines or plant parts derived from petunia-calibrachoa SAKPXC023, and the plants, varieties, and their parts derived from use of those methods. Embodiments further include hybrid seeds, plants, and plant parts produced by crossing petunia-calibrachoa SAKPXC023 with another variety. ## Claims ### Claim 1 A plant of petunia-calibrachoa variety SAKPXC023, wherein a representative sample of plant tissue of SAKPXC023 was deposited under NCMA No. 201907002. ### Claim 2 (depends on 1) A plant, or a plant part thereof produced by growing the plant of claim 1, wherein the plant or plant part comprises at least one cell of petunia-calibrachoa variety SAKPXC023. ### Claim 3 (depends on 1) A petunia-calibrachoa plant, or part thereof, having all of the physiological and morphological characteristics of the petunia-calibrachoa plant of claim 1. ### Claim 4 (depends on 1) A tissue or cell culture of regenerable cells produced from the plant of claim 1. ### Claim 5 (depends on 4) The tissue or cell culture of claim 4, comprising tissues or cells from a plant part selected from the group consisting of leaves, pollen, embryos, cotyledons, hypocotyl, meristematic cells, roots, root tips, pistils, anthers, flowers, and stems. ### Claim 6 (depends on 5) A petunia-calibrachoa plant regenerated from the tissue or cell culture of claim 5, wherein said plant has all of the morphological and physiological characteristics of petunia-calibrachoa SAKPXC023 listed in Table 1. ### Claim 7 (depends on 1) A method of vegetatively propagating the plant of claim 1, comprising the steps of: collecting tissue or cells capable of being propagated from said plant; cultivating said tissue or cells to obtain proliferated shoots; and rooting said proliferated shoots to obtain rooted plantlets; or cultivating said tissue or cells to obtain proliferated shoots, or to obtain plantlets. ### Claim 8 (depends on 7) A petunia-calibrachoa plant produced by growing the plantlets or proliferated shoots of claim 7. ### Claim 9 (depends on 1) A method for producing an embryo or seed, wherein the method comprises crossing the plant of claim 1 with another plant and harvesting the resultant embryo or seed. ### Claim 10 (depends on 1) A method of determining the genotype of the petunia-calibrachoa plant of claim 1, wherein said method comprises obtaining a sample of nucleic acids from said plant and detecting in said nucleic acids a plurality of polymorphisms. ### Claim 11 (depends on 1) A method of producing a petunia-calibrachoa plant resistant to the group consisting of herbicides, insecticides, and disease, wherein the method comprises transforming the petunia-calibrachoa plant of claim 1 with a transgene, and wherein said transgene confers resistance to an herbicide, insecticide, or disease. ### Claim 12 (depends on 11) An herbicide, insecticide, or disease resistant plant produced by the method of claim 11. ### Claim 13 (depends on 1) A method for developing a petunia-calibrachoa plant in a plant breeding program, comprising applying plant breeding techniques comprising crossing, recurrent selection, mutation breeding, wherein said mutation breeding selects for a mutation that is spontaneous or artificially induced, backcrossing, pedigree breeding, marker enhanced selection, haploid/double haploid production, or transformation to the petunia-calibrachoa plant of claim 1, or its parts, wherein application of said techniques results in development of a petunia-calibrachoa plant. ### Claim 14 A method of introducing a mutation into the genome of petunia-calibrachoa plant SAKPXC023 said method comprising mutagenesis of the plant, or plant part thereof, wherein said mutagenesis is selected from the group consisting of temperature, long-term seed storage, tissue culture conditions, ionizing radiation, chemical mutagens, or targeting induced local lesions in genomes, and wherein the resulting plant comprises at least one genome mutation. ### Claim 15 A method of editing the genome of petunia-calibrachoa plant SAKPXC023, said method comprising editing the genome of the plant, or plant part thereof, wherein a representative sample of plant tissue of SAKPXC023 was deposited under NCMA No. 201907002, wherein said method is selected from the group comprising zinc finger nucleases, transcription activator-like effector nucleases (TALENs), engineered homing endonucleases/meganucleases, and the clustered regularly interspaced short palindromic repeat (CRISPR)-associated protein9 (Cas9) system. ### Claim 16 (depends on 15) A petunia-calibrachoa plant produced by the method of claim 15. ### Claim 17 (depends on 1) A petunia-calibrachoa seed produced by growing the plant of claim 1. ### Claim 18 (depends on 17) A method of producing a petunia-calibrachoa plant, or part thereof, produced by growing the seed of claim 17. ### Claim 19 (depends on 9) The method of claim 9, further comprising producing a plant, or a part thereof, from the resultant embryo or seed.