# US 10,499,585 B2 · Hybrid squash plant named Chabela

> Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt

- Number: US 10,499,585 B2
- Document ID: US10499585B2
- Status: Granted
- Kind: B2
- Application number: 15/919,583
- Filed: 2018-03-13
- Published: 2019-09-19
- Granted: 2019-12-10
- Earliest claimed: 2018-03-13
- Assignees: Hm.Clause, Inc.
- Applicants: Hm.Clause, Inc.
- Inventors: Wei Ouyang
- Web page: https://patentgrep.com/patent/US10499585B2

## CPC

- A01H 6/348: {Cucurbita, e.g. squash or pumpkin}
- A01H 5/08: Fruits
- A01H: NEW PLANTS OR {NON-TRANSGENIC} PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES

## Abstract

A novel hybrid squash plant, designated Chabela is disclosed. The invention relates to the seeds of squash hybrid Chabela, to the plants and plant parts of hybrid squash Chabela, and to methods for producing a squash plant by crossing the hybrid squash Chabela with itself or another squash plant.

## Claims

### Claim 1

A seed of hybrid squash designated Chabela, wherein a representative sample of seed of said hybrid has been deposited under NCIMB No. 43359.

### Claim 2 (depends on 1)

A squash plant, a plant part thereof, or a plant cell thereof, produced by growing the seed of claim 1, wherein a squash plant regenerated from said plant part or plant cell has all of the physiological and morphological characteristics of hybrid Chabela when grown under the same environmental conditions.

### Claim 3 (depends on 2)

The squash plant part, or a plant cell thereof of claim 2, wherein the squash part is selected from the group consisting of a leaf, a flower, a fruit, a cell, a rootstock, and a scion.

### Claim 4

A squash plant or a plant part, or a plant cell thereof having all of the physiological and morphological characteristics of hybrid Chabela when grown under the same environmental conditions, wherein a representative sample of seed of said hybrid has been deposited under NCIMB No. 43359.

### Claim 5 (depends on 2)

A tissue culture of regenerable cells produced from the plant or plant part of claim 2, wherein a plant regenerated from the tissue culture has all of the physiological and morphological characteristics of hybrid Chabela when grown in the same environmental conditions.

### Claim 6 (depends on 5)

A squash plant regenerated from the tissue culture of claim 5, said plant having all the physiological and morphological characteristics of hybrid Chabela when grown under the same environmental conditions wherein a representative sample of seed of said hybrid has been deposited under NCIMB No. 43359.

### Claim 7 (depends on 2)

A method for harvesting a squash fruit, said method comprising a) growing the squash plant of claim 2 to produce a squash fruit, and b) harvesting said squash fruit.

### Claim 8 (depends on 2)

A method for producing a squash seed, said method comprising crossing a first parent squash plant with a second parent squash plant and harvesting the resultant squash seed, wherein said first parent squash plant and/or second parent squash plant is the squash plant of claim 2.

### Claim 9 (depends on 2)

A method for producing a squash seed, said method comprising self-pollinating the squash plant of claim 2 and harvesting the resultant squash seed.

### Claim 10 (depends on 2)

A method of vegetatively propagating the squash plant of claim 2, said method comprising a) collecting part of the plant of claim 2 and b) regenerating a plant from said part.

### Claim 11 (depends on 10)

The method of claim 10 further comprising harvesting a fruit from said plant obtained from step (b) of claim 10.

### Claim 12 (depends on 10)

A plant obtained from the method of claim 10, wherein said plant has all of the physiological and morphological characteristics of hybrid Chabela when grown under the same environmental conditions.

### Claim 13 (depends on 2, 7, 11)

A squash fruit selected from: (1) a squash fruit produced from the plant of claim 2; (2) a squash fruit produced by the method of claim 7, and (3) a squash fruit obtained from the method of claim 11.

### Claim 14 (depends on 2)

A method of producing a squash plant derived from the hybrid squash plant Chabela, the method comprising: (a) self-pollinating the plant of claim 2 at least once to produce a progeny plant derived from the hybrid variety Chabela.

### Claim 15 (depends on 14)

The method of claim 14 further comprising the steps of:
(b) crossing the progeny plant derived from the hybrid squash plant Chabela with itself or a second squash plant to produce a seed of progeny plant of subsequent generation;
(c) growing the progeny plant of the subsequent generation from the seed; and
(d) crossing the progeny plant of the subsequent generation with itself or a second squash plant to produce a squash plant derived from the hybrid squash plant Chabela; and
(e) repeating step b) and/or c) for at least one generation to produce a squash plant derived from the squash hybrid Chabela.

### Claim 16 (depends on 2)

A method of producing a squash plant derived from the hybrid squash plant Chabela, the method comprising; (a) crossing the plant of claim 2 with a second squash plant to produce a progeny plant.

### Claim 17 (depends on 16)

The method of claim 16 further comprising the steps of:
(b) crossing the progeny plant derived from the hybrid squash plant Chabela with itself or a second squash plant to produce a seed of progeny plant of subsequent generation;
(c) growing the progeny plant of the subsequent generation from the seed (d) crossing the progeny plant of the subsequent generation with itself or a second squash plant to produce a squash plant derived from the squash hybrid squash plant Chabela; and
(e) repeating step b) and/or c) to produce a squash plant derived from the hybrid squash plant Chabela.

### Claim 18

A squash plant comprises a single locus conversion and otherwise all of the physiological and morphological characteristics of hybrid squash plant Chabela when grown under the same environmental conditions, wherein a representative sample of seed of said hybrid squash plant has been deposited under NCIMB No. 43359, wherein the single locus conversion confers a desired trait in said plant.

### Claim 19 (depends on 18)

The plant of claim 18, wherein the single locus conversion confers said plant with male sterility, herbicide resistance, insect resistance, resistance to a plant pathogenic disease, or water-stress tolerance.

### Claim 20 (depends on 18)

The plant of claim 18, wherein the single locus conversion is introduced in to the plant by artificial mutagenesis.

### Claim 21 (depends on 18)

The plant of claim 18, wherein the single locus conversion is introduced into the plant by a breeding technique selected from the group consisting of Zinc finger nuclease (ZFN) technology, oligonucleotide directed mutagenesis, cisgenesis, intragenesis, RNA-dependent DNA methylation, reverse breeding, agro-infiltration, Transcription Activation-Like Effector Nuclease (TALENs), CRISPR/Cas system, engineered meganuclease re-engineered homing endonuclease, and DNA guided genome editing.

### Claim 22 (depends on 2)

A method of producing a squash plant, said method comprising grafting a rootstock or a scion of the hybrid squash plant of claim 2 to another squash plant.

### Claim 23 (depends on 2)

A method for producing nucleic acids, the method comprising isolating nucleic acids from the plant of claim 2, or a plant part, or a plant cell thereof.

### Claim 24 (depends on 2)

A method for producing a second squash plant, the method comprising applying plant breeding techniques to the plant or plant part of claim 2 to produce the second squash plant.