# US 10,499,584 B2 · Industrial hemp Cannabis cultivars and seeds with stable cannabinoid profiles

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- Number: US 10,499,584 B2
- Document ID: US10499584B2
- Status: Granted
- Kind: B2
- Application number: 15/452,925
- Filed: 2017-03-08
- Published: 2017-11-30
- Granted: 2019-12-10
- Earliest claimed: 2016-05-27
- Assignees: New West Genetics Inc.
- Applicants: New West Genetics
- Inventors: Richard S. Fletcher; John McKay
- Web page: https://patentgrep.com/patent/US10499584B2

## CPC

- A01H 6/28: Cannabaceae, e.g. cannabis
- A01H 5/12: Leaves
- C12C 7/28: After-treatment {, e.g. sterilisation (C12C11/00 takes precedence)}
- A01H: NEW PLANTS OR {NON-TRANSGENIC} PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- C12C: BEER; PREPARATION OF BEER BY FERMENTATION (ageing or ripening by storing C12H1/22; methods for reducing the alcohol content after fermentation C12H3/00; methods for increasing the alcohol content after fermentation C12H6/00; venting devices for casks, barrels or the like C12L9/00); PREPARATION OF MALT FOR MAKING BEER; PREPARATION OF HOPS FOR MAKING BEER

## Abstract

According to the invention, there is provided novel hemp Cannabis cultivars with THC content of 0.2% by dry weight and a unique terpene profile. This invention thus relates to the seeds of hemp Cannabis cultivars of the invention, to the plants of hemp Cannabis cultivars of the invention, to plant parts of hemp Cannabis cultivars of the invention, to methods for producing a Cannabis cultivar by crossing the hemp Cannabis cultivars of the invention with another Cannabis cultivar, and to methods for producing a Cannabis cultivar containing in its genetic material one or more backcross conversion traits or transgenes and to the backcross conversion Cannabis plants and plant parts produced by those methods.

## Claims

### Claim 1

A non-transgenic Cannabis cultivar designated NWG331 or NWG452 with a THC content of 0.2% or less, wherein a representative sample of seed of said cultivar was deposited under Accession No. NCIMB 43290 or NCIMB 43280.

### Claim 2

Seed of Cannabis cultivar designated NWG331 or NWG452, wherein a representative sample of seed of said cultivar was deposited under Accession No. NCIMB 43290 or NCIMB 43280.

### Claim 3 (depends on 2)

A Cannabis plant, or a part thereof, produced by growing the seed of claim 2.

### Claim 4 (depends on 3)

A tissue culture of cells produced from the plant of claim 3, wherein said cells of the tissue culture are produced from a plant part selected from the group consisting of embryo, meristematic cell, leaf, cotyledon, hypocotyl, stem, root, root tip, pistil, anther, flower, seed and pollen.

### Claim 5 (depends on 3)

A protoplast produced from the plant of claim 3.

### Claim 6 (depends on 4)

A protoplast produced from the tissue culture of claim 4.

### Claim 7 (depends on 4)

A Cannabis plant regenerated from the tissue culture of claim 4, wherein the plant has all of the morphological and physiological characteristics of cultivar NWG331 or NWG452, wherein a representative sample of seed was deposited under Accession No. NCIMB 43290 or NCIMB 43280.

### Claim 8 (depends on 3)

A method for producing a hybrid Cannabis seed, wherein the method comprises crossing the plant of claim 3 with a different Cannabis plant and harvesting the resultant F1 hybrid Cannabis seed.

### Claim 9 (depends on 8)

A hybrid Cannabis seed produced by the method of claim 8.

### Claim 10 (depends on 9)

A hybrid Cannabis plant, or a part thereof, produced by growing said hybrid seed of claim 9.

### Claim 11 (depends on 3)

A method of producing a male sterile Cannabis plant wherein the method comprises transforming the Cannabis plant of claim 3 with a nucleic acid molecule that confers male sterility.

### Claim 12 (depends on 11)

A male sterile Cannabis plant produced by the method of claim 11.

### Claim 13 (depends on 3)

A method for producing an herbicide resistant Cannabis plant wherein the method comprises transforming the Cannabis plant of claim 3 with a transgene, wherein the transgene confers resistance to an herbicide selected from the group consisting of imidazolinone, sulfonylurea, glyphosate, glufosinate, L-phosphinothricin, triazine and benzonitrile.

### Claim 14 (depends on 13)

An herbicide resistant Cannabis plant produced by the method of claim 13.

### Claim 15 (depends on 3)

A method of producing an insect resistant Cannabis plant wherein the method comprises transforming the Cannabis plant of claim 3 with a transgene that confers insect resistance.

### Claim 16 (depends on 15)

An insect resistant Cannabis plant produced by the method of claim 15.

### Claim 17 (depends on 16)

The Cannabis plant of claim 16 wherein the transgene encodes a Bacillus thuringiensis endotoxin.

### Claim 18 (depends on 3)

A method of producing a disease resistant Cannabis plant wherein the method comprises transforming the Cannabis plant of claim 3 with a transgene that confers disease resistance.

### Claim 19 (depends on 18)

A disease resistant Cannabis plant produced by the method of claim 18.

### Claim 20 (depends on 3)

A method of producing a Cannabis plant with a value-added trait, wherein the method comprises transforming the Cannabis plant of claim 3 with a heterologous nucleic acid sequence.

### Claim 21 (depends on 20)

A Cannabis plant with a value-added trait produced by the method of claim 20.

### Claim 22

A Cannabis plant, or a part thereof, having all of the physiological and morphological characteristics of hemp Cannabis cultivars NWG331 or NWG452, wherein a representative sample of seed of the cultivar was deposited under Accession No. NCIMB 43290 or NCIMB 43280.

### Claim 23

A method of introducing a desired trait into hemp Cannabis cultivars NWG331 or NWG452 wherein the method comprises:
a) crossing a NWG331 or NWG452 plant grown from NWG331 or NWG452 seed, wherein a representative sample of seed was deposited under Accession No. NCIMB 43290 or NCIMB 43280, with a plant of another Cannabis cultivar that comprises a desired trait to produce F1 progeny plants, wherein the desired trait is selected from the group consisting of altered terpene or cannabinoid composition, male sterility, herbicide resistance, insect resistance, and resistance to bacterial disease, fungal disease, or viral disease;
b) selecting one or more progeny plants that have the desired trait to produce selected progeny plants;
c) crossing the selected progeny plants with the NWG331 or NWG452 plants to produce backcross progeny plants;
d) selecting for backcross progeny plants that have the desired trait and all of the characteristics of hemp Cannabis cultivars NWG331 or NWG452 listed in Table 1 or Table 3 to produce selected backcross progeny plants; and
e) repeating steps (c) and (d) three or more times in succession to produce selected fourth or higher backcross progeny plants that comprise the desired trait and all of the characteristics of hemp Cannabis cultivars NWG331 or NWG452 listed in Table 1 or Table 3.

### Claim 24 (depends on 23)

A Cannabis plant produced by the method of claim 23, wherein the plant has the desired trait and all of the characteristics of NWG331 or NWG452 hemp Cannabis cultivars listed in Table 1 or Table 3.

### Claim 25 (depends on 24)

The Cannabis plant of claim 24, wherein the desired trait is herbicide resistance and the resistance is conferred to an herbicide selected from the group consisting of imidazolinone, sulfonylurea, glyphosate, glufosinate, L-phosphinothricin, triazine and benzonitrile.

### Claim 26 (depends on 24)

The Cannabis plant of claim 24, wherein the desired trait is insect resistance and the insect resistance is conferred by a transgene encoding a Bacillus thuringiensis endotoxin.

### Claim 27 (depends on 24)

The Cannabis plant of claim 24, wherein the desired trait is male sterility and the trait is conferred by a nucleic acid molecule.

### Claim 28 (depends on 3)

A method of conferring aroma, flavoring, or desired health benefits to a beverage comprising;
preparing said beverage with the Cannabis plant of claim 3 or parts thereof or compositions purified therefrom.

### Claim 29 (depends on 28)

The method of claim 28 wherein said beverage is beer, wine, cider, distilled spirit, hard soda, soft drink, juice, water, or flavored water.

### Claim 30 (depends on 3)

A method of preparing cannabinoid isolates or isolate formulations, wherein the method comprises:
harvesting flower tissue from the plant of claim 3; and
extracting cannabinoids from the flower tissue.

### Claim 31 (depends on 3)

A method of producing a Cannabis plant with a modified terpene or cannabinoid profile wherein the method comprises genetically modifying the Cannabis plant of claim 3 with a nucleic acid molecule that modifies the terpene or cannabinoid profile.

### Claim 32 (depends on 3)

A method of producing a Cannabis plant with a THC content of 0.2% or less comprising the steps of:
(a) crossing the plant of claim 3 with a second Cannabis plant to produce a progeny plant;
(b) crossing the progeny plant of step (a) with itself or the second Cannabis plant in step (a) to produce a seed;
(c) growing a progeny plant of a subsequent generation from the seed produced in step (b);
(d) crossing the progeny plant of a subsequent generation of step (c) with itself or the second Cannabis plant in step (a) to produce a Cannabis plant derived from the Cannabis NWG331 or NWG452 with a THC content of 0.2% or less.

### Claim 33 (depends on 3)

A method for developing a Cannabis plant in a Cannabisplant breeding program, comprising applying plant breeding techniques comprising recurrent selection, backcrossing, pedigree breeding, marker enhanced selection, mutation breeding, or genetic modification to the Cannabis plant of claim 3, or its parts, to develop of a Cannabis plant with a THC content of 0.2% or less.