# US 10,499,577 B2 · White celery

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- Number: US 10,499,577 B2
- Document ID: US10499577B2
- Status: Granted
- Kind: B2
- Application number: 15/837,148
- Filed: 2017-12-11
- Published: 2018-05-03
- Granted: 2019-12-10
- Earliest claimed: 2015-07-03
- Assignees: Rijk Zwaan Zaadteelt En Zaadhandel B.V.
- Applicants: Rijk Zwaan Zaadteelt En Zaadhandel B.V.
- Inventors: Reinier Hendrik Marie Deneer
- Web page: https://patentgrep.com/patent/US10499577B2

## CPC

- A01H 6/064: {Apium graveolens [celery]}
- A01H 1/045: {using molecular markers}
- A01H 5/04: Stems
- A01H 5/12: Leaves
- A23L 19/09: {Mashed or comminuted products, e.g. pulp, purée, sauce, or products made therefrom, e.g. snacks (A23L19/01, A23L19/10, A23L27/63 and A23L21/00 take precedence)}
- A23L 33/105: Plant extracts, their artificial duplicates or their derivatives
- C12Q 1/6895: for plants, fungi or algae
- A23V 2002/00: Food compositions, function of food ingredients or processes for food or foodstuffs
- C12Q 2600/13: Plant traits
- C12Q 2600/158: Expression markers
- A01H: NEW PLANTS OR {NON-TRANSGENIC} PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A23L: FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF (preservation thereof A23B)
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- A23V: INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION

## Abstract

The invention relates to a celery plant (Apium graveolens L. dulce) carrying a genetic determinant that leads to the absence or strong reduction of chlorophyll in the petioles, wherein said determinant is obtainable by introgression from a plant grown from seed of which a representative sample was deposited with the NCIMB under deposit number NCIMB 42428. The invention further relates to markers and the use of markers for the identification of a plant that has an absence or strong reduction of chlorophyll in the petioles. The invention also relates to seeds and other propagation material of the plant and to its progeny as well as to food products that comprise the consumable parts of the plant.

## Claims

### Claim 1

A celery plant (Apium graveolens L. dulce) carrying a genetic determinant that leads to an absence or strong reduction of chlorophyll in the petioles, which genetic determinant is as comprised in an Apium graveolens L. dulce plant, representative seed of which was deposited with the NCIMB under deposit number NCIMB 42428, wherein the genetic determinant can be identified by a marker having SEQ ID NO: 1 and wherein the average total chlorophyll content in the petioles of a plant at 14 weeks after transplanting is <4 μg/g.

### Claim 2 (depends on 1)

The celery plant as claimed in claim 1, wherein the genetic determinant is introgressed from a plant comprising said genetic determinant, representative seed of which was deposited with the NCIMB under deposit number NCIMB 42428.

### Claim 3 (depends on 1)

The celery plant as claimed in claim 1, which has a similar growth and/or vigor as compared to a celery plant that has no reduction of chlorophyll in the petioles.

### Claim 4 (depends on 1)

The celery plant as claimed in claim 1, obtainable by crossing a first celery plant with a second celery plant, wherein at least one of the said plants is grown from seed that carries the genetic determinant, representative seed of which was deposited with the NCIMB under deposit number NCIMB 42428, or a progeny plant thereof, optionally crossing the resulting F1, and selecting for plants that have an absence or strong reduction of chlorophyll in the petioles, optionally followed by one or more additional rounds of crossing and/or selection.

### Claim 5 (depends on 1)

The celery plant as claimed in claim 1, wherein the average total chlorophyll content in the petioles of a plant at 2 weeks after transplanting is lower than 40 μg/g.

### Claim 6

An Apium graveolens L. dulce seed, representative seed of which was deposited with the NCIMB under deposit number NCIMB 42428 wherein a plant that can be grown from the seed comprises a genetic determinant that leads to an absence or strong reduction of chlorophyll in the petioles, wherein the genetic determinant can be identified by a marker having SEQ ID NO: 1 and wherein the average total chlorophyll content in the petioles of a plant at 14 weeks after transplanting is <4 μg/g.

### Claim 7 (depends on 1, 6)

A progeny of an Apium graveolens L. dulce plant as claimed in claim 1, or of a plant grown from the seed as claimed in claim 6, comprising the genetic determinant that leads to an absence or strong reduction of chlorophyll in the petioles as comprised in an Apium graveolens L. dulce plant, representative seed of which was deposited with the NCIMB under deposit number NCIMB 42428.

### Claim 8 (depends on 1, 6)

A propagation material suitable for producing the plant as claimed in claim 1 or a plant grown from the seed as claimed in claim 6, wherein the propagation material is suitable for sexual reproduction or is suitable for vegetative reproduction, or is suitable for tissue cultures of regenerable cells, wherein the plant produced from the propagation material comprises the genetic determinant that leads to an absence or strong reduction of chlorophyll in the petioles as comprised in an Apium graveolens L. dulce plant, representative seed of which was deposited with the NCIMB under deposit number NCIMB 42428.

### Claim 9 (depends on 8)

The propagation material as claimed in claim 8, wherein the propagation material suitable for sexual reproduction is selected from microspores, pollen, ovaries, ovules, embryo sacs and egg cells.

### Claim 10 (depends on 8)

The propagation material as claimed in claim 8, wherein the propagation material suitable for vegetative reproduction is selected from cuttings, roots, stems, cells, protoplasts.

### Claim 11 (depends on 8)

The propagation material as claimed in claim 8, wherein the propagation material suitable for tissue cultures of regenerable cells is selected from leaves, pollen, embryos, cotyledon, hypocotyls, meristematic cells, roots, root tips, anthers, flowers, seeds and stems.

### Claim 12 (depends on 1)

The celery plant as claimed in claim 1, wherein the average total chlorophyll content in the petioles of a plant at 2 weeks after transplanting is lower than 60 μg/g.

### Claim 13 (depends on 1)

The celery plant as claimed in claim 1, wherein the average total chlorophyll content in the petioles of a plant at 2 weeks after transplanting is lower than 80 μg/g.

### Claim 14 (depends on 1)

The celery plant as claimed in claim 1, wherein the average total chlorophyll content in the petioles of a plant at 2 weeks after transplanting is lower than 100 μg/g.