Mutant having ability to overproduce carotenoids and method for producing carotenoids by using same
AssigneeAjou University Industry-Academic Cooperation Foundation
InventorsPyung Cheon Lee, Bo Hyun Choi, Sun Hyoung Choi
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Abstract
Provided is a mutant having an ability to overproduce carotenoids and a method for producing carotenoids by using the mutant. The mutant, of which mutations are induced by irradiation after being transformed with a recombinant vector according to the subject matter, has an excellent ability to produce carotenoids and can be mass-produced, and thus can be useful in various industrial fields, which use carotenoids, such as cosmetics, food, and feed.
Claims5
A mutant strain Escherichia coli whose accession number is KCTC 12683BP having an ability to over-produce a carotenoid, which is mutagenized by 60Co gamma-ray source irradiation,
- wherein the mutant strain includes a CrtE gene which encodes geranylgeranyl pyrophosphate synthase and consists of the nucleotide sequence set forth in SEQ ID NO:
- 1;
- a CrtB gene which encodes phytoene synthase and consists of the nucleotide sequence set forth in SEQ ID NO:
- 2;
- a CrtI gene which encodes phytoene desaturase and consists of the nucleotide sequence set forth in SEQ ID NO:
- 3;
- a CrtY gene which encodes lycopene beta cyclase and consists of the nucleotide sequence set forth in SEQ ID NO:
- 4;
- a CrtX gene which encodes glucosyl transferase and consists of the nucleotide sequence set forth in SEQ ID NO:
- 5;
- and a CrtZ gene which encodes beta-carotene hydroxylase and consists of the nucleotide sequence set forth in SEQ ID NO:
- 6, and is transformed with a recombinant vector for production of a carotenoid.
Dependencies are inferred from the claim text.