Cellobiose phosphorylase
AssigneePfeifer & Langen
InventorsTimo Johannes Koch, Thomas Hässler, Birgit Brucher, Andreas Vogel
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Abstract
The invention relates to a cellobiose phosphorylase, which catalyzes, among other things, the synthesis of cellobiose from glucose 1-phosphate and glucose. The cellobiose phosphorylase according to the invention can be understood as a mutation of the cellobiose phosphorylase from Cellulomonas uda. In comparison to cellobiose phosphorylase of the wild type, the cellobiose phosphorylase according to the invention is distinguished by improved activity and process stability, in particular temperature stability, and lower product inhibition and therefore is especially suitable for use in industrial processes.
Claims10
A cellobiose phosphorylase comprising an amino acid sequence which has at least 94% sequence identity to the amino acid sequence set forth in SEQ ID NO:
- 21;
- wherein the amino acid sequence has one or more amino acid mutations selected from the group consisting of Q161M, S169V, R1 88K, D196N, A220L, K283A, A512V, L705T and T788V compared to SEQ ID NO:
- 1;
- and
- wherein the amino acid sequence does not comprise a K283H amino acid mutation.
Dependencies are inferred from the claim text.