Back to results
US 10,494,613 B2Granted
Generation of infectious influenza viruses from virus-like particles
AssigneeWisconsin Alumni Research Foundation (WARF)
InventorsYoshihiro Kawaoka, Gabriele Neumann
Life of this patent
Estimating the term…
Abstract
Compositions and methods to prepare influenza virus-like particles (VLPs) are provided.
Claims6
A method to prepare influenza A or B virus comprising:
- infecting a host cell with two different VLPs, wherein a first isolated VLP has at least one and up to eight influenza A virus segments and a second VLP has at least one and up to eight influenza A virus viral segments, wherein the first VLP and the second VLP comprise one or up to eight influenza A virus viral segments selected from:
- an influenza virus PA segment;
- an influenza virus PB1 segment;
- an influenza virus PB2 segment;
- an influenza virus NP segment;
- an influenza virus M segment;
- an influenza virus NS segment;
- an influenza virus NA segment;
- or
- an influenza virus HA segment or a modified HA segment having sequences for a non-influenza host cell binding protein;
- or
- wherein the first VLP and the second VLP comprise one or up to eight influenza B virus viral segments selected from:
- an influenza virus PA segment;
- an influenza virus PB1 segment;
- an influenza virus PB2 segment;
- influenza virus NP segment;
- an influenza virus M segment;
- an influenza virus NS segment;
- an influenza virus NA segment;
- or
- an influenza virus HA segment or a modified HA segment having sequences for a non-influenza host cell binding protein;
- and
- wherein the first VLP and the second VLP each comprise PA, PB1 PB2, NP, and HA or a non-influenza host cell binding protein;
- wherein the first VLP has at least one viral segment that is not present in the second VLP or wherein the first VLP has at least one segment that is modified in the second VLP so that a functional influenza virus protein is not expressed from that modified segment;
- and
- isolating progeny virus.
23 more elements
Dependencies are inferred from the claim text.