Viral RNA segments as immunomodulatory agents and vaccine components
AssigneeUniversity of Iowa Research Foundation
InventorsJack T. Stapleton, Nirjal Bhattarai, James McLinden, Jinhau Xiang, Bev Davidson
Life of this patent
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Abstract
The disclosure provides compositions and methods involving viral RNA segments for use in modulating immune responses, including inhibition inflammation related to pathogenic T-cell activation. In addition, modification of the viral sequences responsible for modulating immune response provides for improved vaccine formulations.
Claims20
A method of inhibiting immune cell activation comprising administering to a mammalian subject in need thereof a viral RNA segment comprising a nucleic acid encoding a T cell immune-inhibitory domain selected from the group consisting of HCV E2 sequences, YFV envelope, and HIV gp41 sequences, wherein said nucleic acid inhibits said immune cell activation.
A method of performing gene transfer into a subject comprising administering to said subject:
- a) an expression cassette comprising a heterologous gene segment under the control of a promoter operable in cells of said subject and
- b) a viral RNA segment comprising a nucleic acid sequence encoding a T cell immune-inhibitory domain selected from the group consisting of HCV E2 sequences, YFV envelope, and HIV gp41 sequences, wherein said nucleic acid inhibits immune cell activation.
A pharmaceutical composition comprising a nucleic acid having the sequence:
- (SEQ ID NO:
- 3)
- CTCACGCCAAGGTGCCTGATCGACTACCCCTACAGGCTCTGGCATTATCC;
- (SEQ ID NO:
- 4)
- CTCACGCCAAGGTGCCTGATCGACTACCCCTACAGGCTCTGGCATTACCCC;
- or
- (SEQ ID NO:
- 5)
- GACAACAACCTTTACAAACTACATGGT.
- or comprising a nucleic acid sequence selected from GCATTATCC (SEQ ID NO:
- 38), GCAUUAUCC (SEQ ID NO:
- 39), GCATTACCCC (SEQ ID NO:
- 40), GCAUUACCCC (SEQ ID NO:
- 41), CUUUACAAAA (SEQ ID NO:
- 42), or CTTTACAAA (SEQ ID NO:
- 43), wherein said nucleic acid consists essentially of at least about 20 base pairs and no more than about 55 base pairs.
Dependencies are inferred from the claim text.