Microorganisms and methods for the production of butadiene using acetyl-CoA

A non-naturally occurring microbial organism, said microbial organism having a butadiene pathway and comprising at least four exogenous nucleic acids encoding butadiene pathway enzymes expressed in a sufficient amount to produce butadiene, wherein said butadiene pathway comprises a pathway selected from:

• (1) 1A, 1B, 1C, 1G, 1I, 1L, 1M, and 1F;
• (2) 1A, 1B, 1C, 1G, 1I, 1L, 1N, and 1F;
• (3) 1A, 1B, 1C, 1H, 1I, 1L, 1M, and 1F;
• (4) 1A, 1B, 1C, 1H, 1I, 1L, 1N, and 1F;
• (5) 1A, 1B, 1C, 1D, 1J, 1L, 1M, and 1F;
• (6) 1A, 1B, 1C, 1D, 1J, 1L, 1N, and 1F;
• (7) 1A, 1B, 1C, 1D, 1K, 1L, 1M, and 1F;
• and
• (8) 1A, 1B, 1C, 1D, 1K, 1L, 1N, and 1F,
• wherein 1A is an acetaldehyde dehydrogenase, wherein 1B is a 4-hydroxy 2-oxovalerate aldolase, wherein 1C is a 4-hydroxy 2-oxovalerate dehydratase, wherein 1D is a 2-oxopentenoate reductase, wherein 1F is a 2,4-pentadienoate decarboxylase, wherein 1G is a 2-oxopentenoate ligase, wherein 1H is a 2-oxopentenoate:
• acetyl CoA transferase, wherein 1I is a 2-oxopentenoyl-CoA reductase, wherein 1J is a 2-hydroxypentenoate ligase, wherein 1K is a 2-hydroxypentenoate:
• acetyl-CoA CoA transferase, wherein 1L is a 2-hydroxypentenoyl-CoA dehydratase, wherein 1M is a 2,4-Pentadienoyl-CoA hydrolase, and wherein 1N is a 2,4-Pentadienoyl-CoA:
• acetyl CoA transferase.