# US 10,483,096 B2 · Device and method for analysis of biofluids by ion generation using wetted porous material > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 10,483,096 B2 - Document ID: US10483096B2 - Status: Granted - Kind: B2 - Application number: 15/549,948 - Filed: 2016-02-10 - Published: 2018-02-01 - Granted: 2019-11-19 - Earliest claimed: 2015-02-10 - Assignees: Indiana University Research and Technology Corporation - Applicants: Indiana University Research and Technology Corporation - Inventors: Nicholas Manicke; Chengsen Zhang - Web page: https://patentgrep.com/patent/US10483096B2 ## CPC - H01J 49/0413: {for automated handling} - G01N 1/405: {by adsorption or absorption} - G01N 1/4055: {by solubility techniques} - G01N 27/623: combined with mass spectrometry - G01N 30/95: Detectors specially adapted therefor; Signal analysis - H01J 49/0031: {Step by step routines describing the use of the apparatus (H01J49/0081 takes precedence)} - H01J 49/0431: {for liquid samples (interfaces to liquid chromatographs G01N30/7233)} - H01J 49/168: {field ionisation, e.g. corona discharge (atmospheric pressure corona discharge per se H01T19/00)} - G01N 30/72: Mass spectrometers {(mass spectrometers per se H01J49/00)} - G01N 2001/4061: {Solvent extraction} - G01N 2030/009: {Extraction} - H01J: ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS (spark-gaps H01T; electric arc lamps H05B31/00; particle accelerators H05H) - G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q) ## Abstract The present disclosure provides a mass spectrometry cartridge and methods of use thereof. ## Claims ### Claim 1 A mass spectrometry cartridge comprising: a sample holder; a base; a solid phase extraction column, wherein the solid phase extraction column is disposed within the sample holder; and a first absorbent unit, wherein the first absorbent unit is configured for use with a mass spectrometer. ### Claim 2 (depends on 1) The mass spectrometry cartridge according to claim 1, further comprising a second absorbent unit disposed within the base. ### Claim 3 (depends on 2) The mass spectrometry cartridge according to claim 2, wherein the sample holder is slidably disposable within the base. ### Claim 4 (depends on 3) The mass spectrometry cartridge according to claim 3, wherein the sample holder is slidably disposable between a first extraction position, in which the solid phase extraction column is disposed above the second absorbent unit, and a second elution position, in which the solid phase extraction column is disposed above the first absorbent unit. ### Claim 5 (depends on 1) The mass spectrometry cartridge according to claim 1, wherein the sample holder is slidably disposable within the base. ### Claim 6 (depends on 1) The mass spectrometry cartridge according to claim 1, further comprising a cover, wherein the cover is disposed above the solid phase extraction column. ### Claim 7 (depends on 1) The mass spectrometry cartridge according to claim 1, wherein the solid phase extraction column is configured for at least one sample selected from the group consisting of: blood, plasma, urine, bile, water, liquid foodstuffs, and mixtures thereof. ### Claim 8 (depends on 1) The mass spectrometry cartridge according to claim 1, wherein the base is configured to allow an electrical potential to reach the first absorbent unit. ### Claim 9 (depends on 8) The mass spectrometry cartridge according to claim 8, wherein the base comprises a wire. ### Claim 10 (depends on 9) The mass spectrometry cartridge according to claim 9, wherein the sample holder comprises a metallic contact. ### Claim 11 (depends on 10) The mass spectrometry cartridge according to claim 10, wherein the metallic contact is configured to allow an electrical potential to reach the base. ### Claim 12 (depends on 1) The mass spectrometry cartridge according to claim 1, further comprising: a collection disc with an internal standard; and a semi-permeable membrane. ### Claim 13 (depends on 1) The mass spectrometry cartridge according to claim 1, wherein the solid phase extraction column comprises water-wettable material. ### Claim 14 (depends on 1) The mass spectrometry cartridge according to claim 1, wherein the sample holder comprises a metallic contact. ### Claim 15 (depends on 1) The mass spectrometry cartridge according to claim 1, further comprising a protective handle. ### Claim 16 (depends on 15) The mass spectrometry cartridge according to claim 15, wherein the protective handle comprises a prong. ### Claim 17 (depends on 1) The mass spectrometry cartridge according to claim 1, wherein the solid phase extraction column comprises material suitable for protein preconcentration. ### Claim 18 (depends on 17) The mass spectrometry cartridge according to claim 17, wherein the material suitable for protein preconcentration comprises antibody derivatized magnetic beads. ### Claim 19 (depends on 18) The mass spectrometry cartridge according to claim 18, wherein the antibody derivatized magnetic beads are configured to couple to a protein analyte. ### Claim 20 (depends on 17) The mass spectrometry cartridge according to claim 17, wherein the material suitable for protein preconcentration comprises at least one of nitrocellulose, monoclonal antibodies, polyclonal antibodies, aptamers, or combinations thereof. ### Claim 21 A method for analyzing a sample comprising: adding a sample to a cartridge, wherein the cartridge comprises a sample holder, a base, a solid phase extraction column, wherein the solid phase extraction column is disposed within the sample holder, a first absorbent unit, wherein the first absorbent unit is configured for use with a mass spectrometer, and a second absorbent unit disposed within the base; disposing the sample holder in a first extraction position, in which the solid phase extraction column is disposed above the second absorbent unit; disposing the sample holder in a second elution position, in which the solid phase extraction column is disposed above the first absorbent unit; positioning the first absorbent unit in front of a mass spectrometer pressure inlet; applying an electrical potential to the first absorbent unit; and analyzing the sample by mass spectrometry. ### Claim 22 (depends on 21) The method according to claim 21, wherein the sample holder is slidably disposable within the base. ### Claim 23 (depends on 21) The method according to claim 21, wherein the cartridge further comprises a cover, the cover being disposed above the solid phase extraction column. ### Claim 24 (depends on 21) The method according to claim 21, wherein the solid phase extraction column is configured for at least one sample selected from the group consisting of: blood, plasma, urine, bile, water, liquid foodstuffs, and mixtures thereof. ### Claim 25 (depends on 21) The method according to claim 21, wherein the cartridge further comprises: a collection disc with an internal standard; and a semi-permeable membrane. ### Claim 26 (depends on 21) The method according to claim 21, further comprising analyzing the sample by high field asymmetric waveform ion mobility spectrometry. ### Claim 27 (depends on 26) The method according to claim 26, wherein a commercial high field asymmetric waveform ion mobility spectrometry instrument is modified to allow for controlled introduction of gas-phase modifiers. ### Claim 28 (depends on 21) The method according to claim 21, wherein the solid phase extraction column comprises water-wettable material. ### Claim 29 (depends on 21) The method according to claim 21, further comprising adding an elution solvent to the solid phase extraction column. ### Claim 30 (depends on 29) The method according to claim 29, further comprising drying the solid phase extraction column. ### Claim 31 (depends on 29) The method according to claim 29, further comprising adding water to the solid phase extraction column. ### Claim 32 (depends on 31) The method according to claim 31, wherein adding water to the solid phase extraction column occurs before adding an elution solvent to the solid phase extraction column. ### Claim 33 A method for analyzing a sample, comprising: (i) adding a sample to a cartridge, wherein the cartridge comprises (a) a sample holder, (b) a base, (c) a solid phase extraction column, wherein the solid phase extraction column is disposed within the sample holder, (d) a solvent port, wherein the solvent port is disposed within the sample holder, and (e) an absorbent unit, wherein the absorbent unit is configured for use with a mass spectrometer; (ii) adding a solvent to the solvent port; (iii) positioning the absorbent unit in front of mass spectrometer pressure inlet; (iv) applying an electrical potential to the absorbent unit; and (v) analyzing the sample by mass spectrometry. ### Claim 34 A mass spectrometry cartridge, comprising: a sample holder; a base; a solid phase extraction column, wherein the solid phase extraction column is disposed within the sample holder; a solvent port, wherein the solvent port is disposed within the sample holder; and an absorbent unit, wherein the absorbent unit is configured for use with a mass spectrometer.