# US 10,481,169 B2 · Diagnostic method and biomarker for lifestyle disease

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- Number: US 10,481,169 B2
- Document ID: US10481169B2
- Status: Granted
- Kind: B2
- Application number: 15/906,032
- Filed: 2018-02-27
- Published: 2018-08-30
- Granted: 2019-11-19
- Earliest claimed: 2017-02-28
- Assignees: Toyota Jidosha Kabushiki Kaisha; National University Corporation Nagoya University
- Applicants: Toyota Jidosha Kabushiki Kaisha; National University Corporation Nagoya University
- Inventors: Koji Uchida; Takahiro Shibata; Kenji Ishikawa; Oi Lun Helena Li; Tadashi Matsushita; Ryosuke Kikuchi; Junzo Ukai
- Web page: https://patentgrep.com/patent/US10481169B2

## CPC

- G01N 33/92: involving lipids, e.g. cholesterol {, lipoproteins, or their receptors (steroid hormones G01N33/743)}
- H01J 49/0027: {Methods for using particle spectrometers}
- H01J 49/165: {Electrospray ionisation}
- H01J 49/4215: {Quadrupole mass filters (H01J49/4225 takes precedence)}
- G01N 2405/00: Assays, e.g. immunoassays or enzyme assays, involving lipids
- G01N 2560/00: Chemical aspects of mass spectrometric analysis of biological material
- G01N 2800/042: Disorders of carbohydrate metabolism, e.g. diabetes, glucose metabolism
- G01N 2800/044: Hyperlipemia or hypolipemia, e.g. dyslipidaemia, obesity
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)
- H01J: ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS (spark-gaps H01T; electric arc lamps H05B31/00; particle accelerators H05H)

## Abstract

Provided is a novel method and the like for diagnosing a lifestyle disease. The method for diagnosing a lifestyle disease provided by the present invention includes: collecting a biological sample from a subject; measuring the concentration of a fatty acid amide contained in the biological sample; determining whether the subject suffers from a lifestyle disease or a lifestyle disease has progressed when the measured value of the concentration of the fatty acid amide in the sample obtained from the subject is lower than a measurement result obtained from a healthy subject.

## Claims

### Claim 1

A method for diagnosing a lifestyle disease comprising:
preparing a measurement result of a concentration of a fatty acid amide obtained from a healthy subject;
collecting a biological sample from a subject;
measuring a concentration of the fatty acid amide contained in the biological sample; and
determining whether the subject suffers from a lifestyle disease or a lifestyle disease has progressed when a measured value of the concentration of the fatty acid amide obtained from the subject is lower than the measurement result obtained from the healthy subject.

### Claim 2 (depends on 1)

The method for diagnosing a lifestyle disease according to claim 1, wherein the biological sample is an organ or blood.

### Claim 3 (depends on 1)

The method for diagnosing a lifestyle disease according to claim 1, wherein the concentration of the fatty acid amide is measured by liquid chromatography-mass spectrometry.

### Claim 4 (depends on 3)

The method for diagnosing a lifestyle disease according to claim 3, wherein the liquid chromatography-mass spectrometry includes
isolating two or more components contained in the biological sample by liquid chromatography,
ionizing the isolated components and selecting a specific mass component with a quadrupole mass spectrometer, then dissociating the specific mass component, and
detecting the dissociated component with a mass spectrometer.

### Claim 5 (depends on 1)

The method for diagnosing a lifestyle disease according to claim 1, wherein the lifestyle disease is at least one selected from the group consisting of obesity, dyslipidemia, diabetes and impaired glucose tolerance.

### Claim 6 (depends on 1)

The method for diagnosing a lifestyle disease according to claim 1, wherein the fatty acid amide is oleamide.