# US 10,481,163 B1 · Mass spectrometry method using mixed matrix

> Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt

- Number: US 10,481,163 B1
- Document ID: US10481163B1
- Status: Granted
- Kind: B1
- Application number: 15/993,742
- Filed: 2018-05-31
- Published: 2019-12-05
- Granted: 2019-11-19
- Earliest claimed: 2018-05-31
- Assignees: Shimadzu Corporation
- Applicants: Shimadzu Corporation
- Inventors: Yuko Fukuyama; Koichi Tanaka
- Web page: https://patentgrep.com/patent/US10481163B1

## CPC

- G01N 33/6851: {Methods of protein analysis involving laser desorption ionisation mass spectrometry}
- H01J 49/0027: {Methods for using particle spectrometers}
- H01J 49/0418: {for laser desorption, e.g. matrix-assisted laser desorption/ionisation [MALDI] plates or surface enhanced laser desorption/ionisation [SELDI] plates}
- H01J 49/164: {Laser desorption/ionisation, e.g. matrix-assisted laser desorption/ionisation [MALDI] (sample holders H01J49/0418)}
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)
- H01J: ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS (spark-gaps H01T; electric arc lamps H05B31/00; particle accelerators H05H)

## Abstract

The present invention provides a mass spectrometry method using a mixed matrix, capable of easily and efficiently improving the ionization efficiency in mass spectrometry for peptides having a wide range of degrees of hydrophobicity (from hydrophilic to hydrophobic). A MALDI mass spectrometry method for analyzing a sample using as a mixed matrix, 2,4,6-trihydroxyalkylphenone represented by the following general formula (I): wherein R represents an alkyl group having 3 to 12 carbon atoms, and a matrix (II) for mass spectrometry that is more hydrophilic than 2,4,6-trihydroxyalkylphenone represented by the formula (I) wherein R is an alkyl group having 3 carbon atoms.

## Claims

### Claim 1

A MALDI mass spectrometry method for analyzing a sample using as a mixed matrix,
2,4,6-trihydroxyalkylphenone represented by the following general formula (I):
wherein R represents an alkyl group having 3 to 12 carbon atoms, and
a matrix (II) for mass spectrometry that is more hydrophilic than 2,4,6-trihydroxyalkylphenone represented by the formula (I) wherein R is an alkyl group having 3 carbon atoms.

### Claim 2 (depends on 1)

The mass spectrometry method according to claim 1, wherein the compound represented by the general formula (I) is 1-(2,4,6-trihydroxyphenyl)octan-1-one having the following structure:

### Claim 3 (depends on 1)

The mass spectrometry method according to claim 1, wherein the matrix (II) for mass spectrometry is selected from the group consisting of α-cyano-4-hydroxycinnamic acid (4-CHCA), 2,5-dihydroxybenzoic acid (DHB), and 2,4,6-trihydroxyacetophenone (THAP).

### Claim 4 (depends on 1)

The mass spectrometry method according to claim 1, wherein a hydrophobic focus plate is used.

### Claim 5 (depends on 1)

The mass spectrometry method according to claim 1, wherein a sample to be analyzed is a mixture sample containing a peptide and a compound other than peptide.

### Claim 6 (depends on 1)

The mass spectrometry method according to claim 1, wherein the sample to be analyzed is a mixture sample containing a plurality of peptides having different hydrophobicity. The term peptide includes protein.

### Claim 7 (depends on 1)

The mass spectrometry method according to claim 1, wherein the sample to be analyzed is a mixture sample containing a protein digest.