# US 10,481,138 B2 · Chromatogram data processing device and processing method

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- Number: US 10,481,138 B2
- Document ID: US10481138B2
- Status: Granted
- Kind: B2
- Application number: 14/772,432
- Filed: 2014-02-12
- Published: 2016-02-04
- Granted: 2019-11-19
- Earliest claimed: 2013-03-04
- Assignees: Shimadzu Corporation
- Applicants: Shimadzu Corporation
- Inventors: Kenichi Mishima; Etsuho Kamata; Hiroshi Miura; Yasuhiro Mito; Toshinobu Yanagisawa
- Web page: https://patentgrep.com/patent/US10481138B2

## CPC

- G01N 30/74: Optical detectors {(measurement of intensity, velocity, spectral content, polarisation, or phase of infrared, visible or ultraviolet light G01J)}
- G01N 30/86: Signal analysis
- G01N 30/8624: {Detection of slopes or peaks; baseline correction}
- G01N 30/8675: {Evaluation, i.e. decoding of the signal into analytical information (for analysis of specific compounds see also G01N30/88 and subgroups of G01N33/00; chemical libraries per se C40B)}
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)

## Abstract

Regarding a chromatogram data processing device configured to process three-dimensional chromatogram data collected on a target sample in which dimensions are made up of time, wavelength, and absorbance, and the chromatogram data processing device includes a differential spectrum generating means configured to generate a differential spectrum that represents a change in a wavelength differential coefficient, which is a differential coefficient in a wavelength direction in a predetermined wavelength range, based on the three-dimensional chromatogram data, with respect to an absorbance spectrum representing a relation of the wavelength and the absorbance at each time in an entire temporal range or a predetermined temporal range, and a determination means configured to determine whether or not one or plural other components are included in a peak of a target component, based on a temporal change in a waveform of the differential spectrum, so that the determination on whether or not a target sample includes impurities can be performed with high accuracy without the requirement of complicated computation processing.

## Claims

### Claim 1

A system, comprising:
a chromatograph including a column that separates components of a target sample and a detector that detects the separated components;
a chromatogram data processing device configured to process three-dimensional chromatogram data collected on the detected separated components of the target sample in which dimensions are made up of time, wavelength, and absorbance, and generate an absorbance spectrum representing a relation of the wavelength and the absorbance;
a display configured to display a window of the absorbance spectrum;
an operating unit configured to receive a wavelength designated on the absorbance spectrum displayed by an operator,
wherein the chromatogram data processing device is configured to act as:
a computing unit configured to calculate a specific wavelength closest to the wavelength designated by the operator, the specific wavelength being either one of a local maximum absorption wavelength and a local minimum absorption wavelength of the target sample at which a differential coefficient reaches zero on the absorbance spectrum;
a differential chromatogram generating unit configured to calculate a differential coefficient at the specific wavelength of the absorbance spectrum obtained at each time and configured to generate a differential spectrum at each time that represents a change in the differential coefficient at the specific wavelength; and
a determination unit configured to determine whether or not one or plural other components are included a peak of a target component, based on the change in the differential coefficient at each time, and whether or not the target sample includes impurities.

### Claim 2 (depends on 1)

The system according to claim 1,
wherein the determination unit is configured to find a wavelength at which the differential coefficient at each time reaches zero, and configured to determine whether or not one or plural other components are included in the peak of the target component, based on a temporal change in the wavelength.

### Claim 3 (depends on 2)

The system according to claim 2, wherein the display is configured to display the temporal change in the wavelength at which the differential coefficient at each time reaches zero.

### Claim 4

A method, comprising:
separating components of a target sample using a column of a chromatograph and detecting the separated components;
processing, by a data processing device, three-dimensional chromatogram data collected on the detected separated components of the target sample in which dimensions are made up of time, wavelength, and absorbance, and generating an absorbance spectrum representing a relation of the wavelength and the absorbance;
displaying a window of the absorbance spectrum;
receiving a wavelength designated on the absorbance spectrum displayed by an operator,
wherein the processing comprises:
calculating a specific wavelength closest to the wavelength designated by the operator, the specific wavelength being either one of a local maximum absorption wavelength and a local minimum absorption wavelength of the target sample at which a differential coefficient reaches zero on the absorbance spectrum;
calculating a differential coefficient at the specific wavelength of the absorbance spectrum obtained at each time;
generating a differential spectrum at each time that represents a change in the differential coefficient at the specific wavelength; and
determining whether or not one or plural other components are included in a peak of a target component, based on the change in the differential coefficient at each time, and whether or not the target sample includes impurities.