# US 10,480,004 B2 · Brassica plant comprising a mutant ALCATRAZ allele

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- Number: US 10,480,004 B2
- Document ID: US10480004B2
- Status: Granted
- Kind: B2
- Application number: 13/994,173
- Filed: 2011-12-16
- Published: 2013-10-31
- Granted: 2019-11-19
- Earliest claimed: 2010-12-24
- Assignees: BASF Agricultural Solutions Seed US LLC
- Applicants: Benjamin Laga
- Inventors: Benjamin Laga
- Web page: https://patentgrep.com/patent/US10480004B2

## CPC

- C12N 15/8271: {for stress resistance, e.g. heavy metal resistance}
- C12N 15/8218: {Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]}
- C12N 15/8266: {Abscission; Dehiscence; Senescence}
- Y02A 40/146: Genetically Modified [GMO] plants, e.g. transgenic plants
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- Y02A: TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE

## Abstract

The present invention relates to Brassica plants comprising mutant ALC genes, ALC nucleic acid sequences and proteins, as well as methods for generating and identifying said plants and alleles, which can be used to plants with increased podshatter resistance.

## Claims

### Claim 1

A Brassica napus plant, or a cell, part, seed or progeny thereof, comprising two non-naturally occurring full knock-out alc genes, wherein one non-naturally occurring full knock-out alc gene is a mutated version of the native ALC gene comprising at least 98% sequence identity to SEQ ID NO: 3 or encoding an amino acid sequence comprising at least 98% sequence identity to SEQ ID NO: 9; and wherein one non-naturally occurring full knock-out alc gene is a mutated version of the native ALC gene comprising at least 98% sequence identity to SEQ ID NO: 4 or encoding an amino acid sequence comprising at least 98% sequence identity to SEQ ID NO: 10;
wherein said full knock-out mutant alc gene comprises a mutated DNA region consisting of one or more inserted, deleted or substituted nucleotides compared to a corresponding wild-type DNA region in the functional ALC gene and wherein said mutant alc allele does not encode a functional ALC protein,
wherein the podshatter resistance is increased as compared to the podshatter resistance of a corresponding plant not comprising full knock-out alc genes.

### Claim 2 (depends on 1)

A plant according to claim 1, wherein one non-naturally occurring full knock-out alc gene is an ALC gene from the A genome containing a mutated splice site characterized by a G to A substitution at position 668 of SEQ ID NO: 3; and wherein one non-naturally occurring full knock-out alc gene is an ALC gene from the C genome containing a premature stopcodon characterized by a C to T substitution at position 646 of SEQ ID NO: 4.

### Claim 3 (depends on 1)

A plant according to claim 1, which is homozygous for the full knock-out alc genes.

### Claim 4 (depends on 1)

A plant according to claim 1, which produces no functional ALC protein.

### Claim 5 (depends on 1)

A plant according to claim 1, which maintains an agronomically relevant threshability of the pods.

### Claim 6 (depends on 1)

A plant according to claim 1, wherein the seed yield of the plant is increased, preferably significantly increased compared to the seed yield of a corresponding plant not comprising non-naturally occurring full knock-out alc genes.

### Claim 7 (depends on 1)

A seed pod obtainable from a plant according to claim 1.

### Claim 8

A full knock-out allele of an ALC gene, wherein the ALC gene from A genome contains a mutated splice site characterized by a G to A substitution at position 668 of SEQ ID NO: 3, and wherein the ALC gene from C genome contains a premature stopcodon characterized by a C to T substitution at position 646 of SEQ ID NO: 4.

### Claim 9 (depends on 1)

A method for identifying the Brassica napus plant, or a cell, part, seed or progeny thereof, of claim 1, said method comprising identifying said two full knock-out alc genes as described in claim 1 in a biological sample comprising determining the presence of a full knock-out alc specific region of each full knock-out gene in a nucleic acid present in the biological sample.