Algal based edible vaccines
AssigneeTransalgae Israel Ltd.
InventorOfra Chen
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Abstract
Provided are edible vaccines including transgenic microalgae expressing at least one exogenous antigen or an intervening organism including the transgenic microalgae. The antigen expressing microalgae are used for oral delivery of the antigen to a target organism in its intact and functional form. The exogenous antigen, expressed in the microalgae, is characterized by exerting at least one immunogenic response in the subject consuming the vaccine.
Claims15
An edible vaccine comprising a transgenic eukaryotic microalga, the transgenic microalga comprising an expression cassette comprising at least one polynucleotide encoding an exogenous antigen further comprising a polynucleotide encoding a vacuolar targeting peptide having the amino acid sequence set forth in any one of SEQ ID NO:1 and SEQ ID NO:9, wherein the encoded exogenous antigen is localized within the vacuole of the microalga cell, and wherein said encoded exogenous antigen induces an immune response in a target subject consuming said vaccine against a disease caused by a pathogen.
A method for oral delivery of an antigen to a subject in need thereof, the method comprising orally administering to the subject an effective amount of a vaccine comprising a transgenic eukaryotic microalga comprising an expression cassette comprising at least one polynucleotide encoding an exogenous antigen further comprising a polynucleotide encoding a vacuolar targeting peptide having the amino acid sequence set forth in any one of SEQ ID NO:1 and SEQ ID NO:9, wherein the encoded exogenous antigen is localized within the vacuole of the microalga cell, and wherein said encoded exogenous antigen induces an immune response in said subject against diseases caused by a pathogen.
A method for oral delivery of an antigen to a target animal subject, the method comprising the steps of:
- (a) orally administering to an intervening organism an effective amount of a transgenic microalga comprising at least one polynucleotide encoding exogenous antigen further comprising a polynucleotide encoding a vacuolar targeting peptide having the amino acid sequence set forth in any one of SEQ ID NO:1 and SEQ ID NO:9, wherein the encoded exogenous antigen is localized within the vacuole of the microalga cell, thereby obtaining an intervening organism that have ingested the transgenic microalga;
- and
- (b) orally administering to the target animal subject an effective amount of the intervening organism that has ingested the transgenic microalga,
- wherein said encoded exogenous antigen induces an immune response in said target animal subject against disease caused by a pathogen.
Dependencies are inferred from the claim text.