# US 10,479,999 B2 · Engineered shell proteins for microcompartment shell electron transfer and catalysis

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- Number: US 10,479,999 B2
- Document ID: US10479999B2
- Status: Granted
- Kind: B2
- Application number: 15/851,382
- Filed: 2017-12-21
- Published: 2018-08-02
- Granted: 2019-11-19
- Earliest claimed: 2016-12-23
- Assignees: Board of Trustees of Michigan State University
- Applicants: Board of Trustees of Michigan State University
- Inventors: Cheryl A. Kerfeld; Clement Aussignargues
- Web page: https://patentgrep.com/patent/US10479999B2

## CPC

- C12N 15/52: Genes encoding for enzymes or proenzymes
- C07K 14/195: from bacteria
- C12N 15/113: Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; {Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing (when used in plants C12N15/8218)}
- C12N 15/65: using markers (enzymes used as markers C12N15/52)
- C12N 15/67: General methods for enhancing the expression
- C12N 15/74: Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C07K 2319/01: containing a localisation/targetting motif
- C07K 2319/735: containing a domain for self-assembly, e.g. a viral coat protein (includes phage display)
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)

## Abstract

Described herein are bacterial microcompartments shell proteins modified to stably incorporate iron-sulfur clusters. Such bacterial microcompartments shell proteins exhibit redox cycling and confer electron transfer functionality to bacterial microcompartment shells.

## Claims

### Claim 1

A bacterial microcompartment comprising a S55C-modified BMC-T1 polypeptide with a cysteine at position 55 and with at least 97% sequence identity to SEQ ID NO:3.

### Claim 2 (depends on 1)

A host cell comprising the bacterial microcompartment of claim 1.

### Claim 3 (depends on 1)

The bacterial microcompartment of claim 1, further comprising at least one BMC-H subunit.

### Claim 4 (depends on 1)

The bacterial microcompartment of claim 1, further comprising at least one BMC-P subunit.

### Claim 5 (depends on 1)

The bacterial microcompartment of claim 1, further comprising one or more BMC-T2 proteins.

### Claim 6 (depends on 1)

The bacterial microcompartment of claim 1, further comprising one or more BMC-T3 proteins.

### Claim 7 (depends on 1)

The bacterial microcompartment of claim 1, further comprising one or more iron atoms.

### Claim 8 (depends on 1)

The bacterial microcompartment of claim 1, further one or more [4Fe-4S] clusters.

### Claim 9 (depends on 1)

The bacterial microcompartment of claim 1, further comprising an enzyme.

### Claim 10 (depends on 9)

The bacterial microcompartment of claim 9, wherein the enzyme catalyzes an oxidation reaction.

### Claim 11 (depends on 1)

The bacterial microcompartment of claim 1, further comprising an enzyme that can catalyze the conversion of NAD/NADP.