# US 10,479,985 B1 · Cellulase having improved enzymatic activity

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- Number: US 10,479,985 B1
- Document ID: US10479985B1
- Status: Granted
- Kind: B1
- Application number: 16/406,255
- Filed: 2019-05-08
- Granted: 2019-11-19
- Earliest claimed: 2019-04-16
- Assignees: Hubei University
- Applicants: Hubei University
- Inventors: Chun-Chi Chen; Jian-Wen Huang; Longhai Dai; Xuejing Yu; Chin-Yu Chen; Shan Wu; Zhichun Zhan; Lilan Zhang; Chao Zhai; Lixin Ma; Rey-Ting Guo
- Web page: https://patentgrep.com/patent/US10479985B1

## CPC

- C12N 9/2437: {Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)}
- C12Y 302/01004: Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- C12Y: ENZYMES

## Abstract

A cellulase having improved enzymatic activity is disclosed. The cellulase has a modified amino acid sequence of SEQ ID NO: 2 or a modified amino acid sequence with at least 80% sequence identity of SEQ ID NO: 2, wherein the modification is a substitution of methionine at position 120 or a corresponding position with asparagine.

## Claims

### Claim 1

A cellulase having a modified amino acid sequence of SEQ ID NO: 2 or a modified amino acid sequence with at least 80% sequence identity of SEQ ID NO: 2, wherein the modification is a substitution of methionine at position 120 or a corresponding position with asparagine.

### Claim 2 (depends on 1)

The cellulase according to claim 1 wherein a gene encoding the amino acid sequence of SEQ ID NO: 2 is FI-CMCase gene isolated from Aspergillus aculeatus F-50.

### Claim 3 (depends on 1)

The cellulase according to claim 1 being an endoglucanase.

### Claim 4 (depends on 1)

The cellulase according to claim 1 having the full length amino acid sequence of SEQ ID NO: 5.

### Claim 5 (depends on 1)

A nucleic acid encoding the cellulase of claim 1.

### Claim 6 (depends on 5)

A recombinant plasmid comprising the nucleic acid of claim 5.