# US 10,479,977 B2 · Osteochondroreticular stem cells for bone and cartilage regeneration

> Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt

- Number: US 10,479,977 B2
- Document ID: US10479977B2
- Status: Granted
- Kind: B2
- Application number: 15/525,208
- Filed: 2015-11-09
- Published: 2017-11-23
- Granted: 2019-11-19
- Earliest claimed: 2014-11-07
- Assignees: The Trustees of Columbia University in the City of New York
- Applicants: The Trustees of Columbia University In the City of New York
- Inventors: Timothy Wang; Daniel Worthley; Siddhartha Mukherjee
- Web page: https://patentgrep.com/patent/US10479977B2

## CPC

- C12N 5/0654: {Osteocytes, Osteoblasts, Odontocytes; Bones, Teeth}
- A61K 39/00: Medicinal preparations containing antigens or antibodies (materials for immunoassay G01N33/53)
- C12N 5/0663: {Bone marrow mesenchymal stem cells (BM-MSC)}
- C12N 5/0669: {Bone marrow stromal cells; Whole bone marrow (isolated stem cells from bone marrow C12N5/0647, C12N5/0663)}
- A61K 35/28: Bone marrow; Haematopoietic stem cells; Mesenchymal stem cells of any origin, e.g. adipose-derived stem cells
- A61K 2039/515: {Animal cells}
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)

## Abstract

The invention is directed to osteochondroreticular stem cells and methods of using osteochondroreticular stem cells. In another aspect the invention is directed to a method of treating osteoarthritis or skeletal fractures using osteochondroreticular stem cells.

## Claims

### Claim 1

A method of treating a disease, degeneration, or injury of bone, or cartilage, or both, in a subject comprising administering a therapeutically effective amount of a composition comprising an acceptable carrier and isolated osteochondroreticular (OCR) stem cells that express Grem1 to a site in need thereof in the subject, wherein the isolated OCR stem cells are at least about 70% pure.

### Claim 2 (depends on 1)

The method of claim 1 wherein the site in need thereof is a joint and the therapeutically effective amount of the composition is administered into a space of the joint or into articular tissue of the joint.

### Claim 3 (depends on 2)

The method of claim 2, wherein the disease treated is osteoarthritis.

### Claim 4 (depends on 1)

The method of claim 1, wherein the site in need thereof is a fracture, and the therapeutically effective amount of the composition is administered into the fracture or tissue surrounding the fracture, or both.