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US 10,479,975 B2Granted
Methods of making T cell compositions
Assigneebluebird bio, Inc.
InventorKevin Friedman
Life of this patent
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Abstract
The invention provides improved T cell compositions and methods for manufacturing T cells. More particularly, the invention provides methods of T cell manufacturing that result in adoptive T cell immunotherapies with improved survival, expansion, and persistence in vivo.
Claims21
A method for manufacturing T cells comprising:
- a) activating a population of T cells and stimulating the population of T cells to proliferate;
- b) transducing the T cells with a viral vector comprising a polynucleotide encoding an engineered T cell receptor (TCR) or a chimeric antigen receptor (CAR);
- and
- c) culturing the transduced T cells to proliferate;
- wherein steps a)-c) are performed in the presence of a phosphatidylinositol-3 kinase (PI3K) inhibitor, and
- wherein T cell differentiation is decreased in the T cells manufactured according to steps a)-c) performed in the presence of the PI3K inhibitor compared to T cell differentiation in T cells manufactured according to steps a)-c) performed in the absence of the PI3K inhibitor.
3 more elements
Dependencies are inferred from the claim text.