# US 10,479,817 B2 · Templates for controlling synthesis of nanoparticles into discrete assemblies

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- Number: US 10,479,817 B2
- Document ID: US10479817B2
- Status: Granted
- Kind: B2
- Application number: 14/931,805
- Filed: 2015-11-03
- Published: 2016-02-25
- Granted: 2019-11-19
- Earliest claimed: 2007-10-19
- Assignees: University of Pittsburgh—Of the Commonwealth System of Higher Education
- Applicants: University of Pittsburgh—Of the Commonwealth System of Higher Education
- Inventors: Nathaniel L. Rosi; Chun-Long Chen
- Web page: https://patentgrep.com/patent/US10479817B2

## CPC

- C07K 7/08: having 12 to 20 amino acids (gastrins C07K14/595; somatostatins C07K14/655; melanotropins C07K14/68)
- B22F 1/054: Nanosized particles
- B22F 1/0551: {Flake form nanoparticles}
- B82Y 30/00: Nanotechnology for materials or surface science, e.g. nanocomposites
- C07K 7/06: having 5 to 11 amino acids
- B22F 2998/00: Supplementary information concerning processes or compositions relating to powder metallurgy
- C22B 11/04: {by wet processes (extraction of metal compounds by leaching in organic solutions C22B3/16; treatment or purification of solutions by liquid-liquid extraction C22B3/26)}
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)
- B22F: WORKING METALLIC POWDER; MANUFACTURE OF ARTICLES FROM METALLIC POWDER; MAKING METALLIC POWDER (making alloys by powder metallurgy C22C); APPARATUS OR DEVICES SPECIALLY ADAPTED FOR METALLIC POWDER
- B82Y: SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- C22B: PRODUCTION AND REFINING OF METALS (electrolytic C25); PRETREATMENT OF RAW MATERIALS

## Abstract

An approach to synthesizing and assembling nanoparticles into discrete, size-tunable, pre-designed architectures is realized in a single synthetic/process step.

## Claims

### Claim 1

A nanostructure comprising: (a) a conjugate molecule comprising a biomineralization peptide attached chemically at its N-terminus to a self-associating moiety, wherein the self-associating moiety is selected from a linear aliphatic carbon chain and an aromatic organic group; and (b) a nanoparticle comprising a metal or semiconductor element; wherein the biomineralization peptide binds to the metal or semiconductor element of the nanoparticle.

### Claim 2 (depends on 1)

The nanostructure according to claim 1, wherein the self-associating moiety is a linear aliphatic carbon chain.

### Claim 3 (depends on 1)

The nanostructure according to claim 1, wherein the self-associating moiety comprises succinimide-activated dodecanoic acid.

### Claim 4 (depends on 1)

The nanostructure according to claim 1, wherein the biomineralization peptide comprises the amino acid sequence AYSSGAPPMPPF (SEQ ID NO: 1).

### Claim 5 (depends on 1)

The nanostructure according to claim 1, wherein the self-associating moiety is a linear aliphatic carbon chain.

### Claim 6 (depends on 1)

The nanostructure according to claim 1, wherein the self-associating moiety is an aromatic organic group.

### Claim 7 (depends on 1)

The nanostructure according to claim 1, wherein the biomineralization peptide exhibits an affinity for nanoparticles comprising gold, iron oxide, silica, silver, zinc sulfide, cadmium sulfide, or cobalt.

### Claim 8 (depends on 7)

The nanostructure according to claim 7, wherein the biomineralization peptide exhibits an affinity for nanoparticles comprising cobalt.

### Claim 9 (depends on 7)

The nanostructure according to claim 7, wherein the biomineralization peptide exhibits an affinity for nanoparticles comprising gold.