# US 10,479,712 B2 · Methods and compositions for nitrogen removal using Feammox microorganisms > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 10,479,712 B2 - Document ID: US10479712B2 - Status: Granted - Kind: B2 - Application number: 15/707,380 - Filed: 2017-09-18 - Published: 2018-02-01 - Granted: 2019-11-19 - Earliest claimed: 2014-05-08 - Assignees: The Trustees of Princeton University - Applicants: The Trustees of Princeton University - Inventors: Peter R. Jaffe; Shan Huang - Web page: https://patentgrep.com/patent/US10479712B2 ## CPC - C02F 3/341: {Consortia of bacteria} - C02F 3/301: {Aerobic and anaerobic treatment in the same reactor} - C02F 3/303: {characterised by the nitrification} - C02F 3/307: {characterised by direct conversion of nitrite to molecular nitrogen, e.g. by using the Anammox process} - C02F 3/346: {Iron bacteria} - C02F 2101/006: {Radioactive compounds} - C02F 2101/16: Nitrogen compounds, e.g. ammonia - C02F 2101/20: Heavy metals or heavy metal compounds - C02F 2101/30: Organic compounds - C02F: TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE (separation in general B01D; special arrangements on waterborne vessels of installations for treating water, waste water or sewage, e.g. for producing fresh water, B63J; adding materials to water to prevent corrosion C23F; treating radioactively-contaminated liquids G21F9/04; regeneration of reactants for recirculation into processes, see the relevant places for the processes) ## Abstract Processes and compositions for removal nitrogen, organic and inorganic contaminants from wastewater using Feammox bacterium are provided. ## Claims ### Claim 1 A composition comprising: an Acidimicrobiaceae Feammox bacterium A6 having Accession Deposit Number PTA-122488, a carrier and at least one of soil or water, wherein the bacterium is capable of oxidizing ammonium coupled with reduction of Fe(III) to Fe(II), the carrier comprises a medium for culturing the bacterium, the at least one of soil and water comprises an ammonium containing contaminant, and the water comprises any water adversely affected in quality by anthropogenic influence. ### Claim 2 (depends on 1) The composition of claim 1, wherein the ammonium containing contaminant is selected from the group consisting of: ammonium chloride, an industrial waste, an agricultural waste, a human municipal waste, fertilizers, domestic sewage, and industrial effluents. ### Claim 3 (depends on 1) The composition of claim 1 further comprising at least one more contaminant selected from the group consisting of: a halogenated organic contaminant and an inorganic contaminant. ### Claim 4 (depends on 3) The composition of claim 3 comprising the halogenated contaminant, and the halogenated organic contaminant comprises at least one compound selected from the group consisting of: perchloroethylene (PCE), trichloroethylene (TCP), trichloroethane, dichloroethane, vinyl chloride, polychlorinated biphenyls, fuel constituents, benzene, ethylbenzene, toluene, xylene, phenanthrene, methyl tent butyl ether, tertiary butyl alcohol, polyaromatic hydrocarbons, and ethylene dibromide. ### Claim 5 (depends on 3) The composition of claim 3 comprising the inorganic contaminant, wherein the inorganic contaminant is selected from the group consisting of: ferric iron, copper, lead, zinc, arsenic, chromium and silver. ### Claim 6 (depends on 3) The composition of claim 3 comprising the inorganic contaminant, and the inorganic contaminant comprises uranium. ### Claim 7 (depends on 1) The composition of claim 1 further comprising a source of ferric iron. ### Claim 8 (depends on 7) The composition of claim 7, wherein the source is selected from the group consisting of: a ferrihydrite, a goethite, a scrap iron, a nontronite, and an iron-rich clay. ### Claim 9 (depends on 1) The composition of claim 1, wherein the medium comprises an iron oxide and has an acidic pH. ### Claim 10 (depends on 1) The composition of claim 1 further comprising CO2. ### Claim 11 (depends on 1) The composition of claim 1, wherein the Acidimicrobiaceae Feammox bacterium A6 is at a concentration of at least 104 bacterial cells per milliliter of the composition. ### Claim 12 A process for treating soil or water comprising mixing an Acidimicrobiaceae Feammox bacterium A6 having Accession Deposit Number PTA-122488 with at least one of the soil or water, wherein the bacterium is capable of oxidizing ammonium coupled with reduction of Fe(III) to Fe(II), the at least one of the soil or water comprises an ammonium containing contaminant, and the water is any water adversely affected in quality by anthropogenic influence. ### Claim 13 (depends on 12) The process of claim 12, wherein the ammonium containing contaminant is selected from the group consisting of: ammonium chloride, an industrial waste, an agricultural waste, or a human municipal waste, fertilizers, domestic sewage, and industrial effluents. ### Claim 14 (depends on 12) The process of claim 12 further comprising removing at least one more contaminant selected from the group consisting of: a halogenated organic contaminant and inorganic contaminant. ### Claim 15 (depends on 14) The process of claim 14, wherein the at least one more contaminant comprises the halogenated organic contaminant, which comprises at least one compound selected from the group consisting of: perchloroethylene (PCE), trichloroethylene (TCE), trichloroethane, dichloroethane, vinyl chloride, polychlorinated biphenyls, fuel constituents, benzene, ethylbenzene, toluene, xylene, phenanthrene, methyl tert butyl ether, tertiary butyl alcohol, polyaromatic hydrocarbons, and ethylene dibromide. ### Claim 16 (depends on 14) The process of claim 14, wherein the at least one or more contaminant comprises the inorganic contaminant, and the inorganic contaminant is selected from the group consisting of: ferric iron, copper, lead, zinc, arsenic, chromium and silver. ### Claim 17 (depends on 14) The process of claim 14, wherein the at least one or more contaminant comprises the inorganic contaminant, and the inorganic contaminant comprises uranium. ### Claim 18 (depends on 14) The process of claim 14 further comprising at least one of creating or maintaining an absence or low level of oxygen, establishing a temperature in a range from 4° C. to 35° C., or establishing a pH in a range from 2.0 to 7.5. ### Claim 19 (depends on 18) The process of claim 18 further comprising adding a source of ferric iron. ### Claim 20 (depends on 19) The process of claim 19, wherein the source of ferric iron is selected from the group consisting of: a ferrihydrite, a goethite, a scrap iron, a nontronite, and an iron-rich clay.