# US 10,479,000 B2 · Method for manufacturing sample storage device and sample storage device

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- Number: US 10,479,000 B2
- Document ID: US10479000B2
- Status: Granted
- Kind: B2
- Application number: 14/420,751
- Filed: 2013-07-19
- Published: 2015-07-23
- Granted: 2019-11-19
- Earliest claimed: 2012-08-10
- Applicants: Seung Kook Yu
- Inventors: Seung Kook Yu
- Web page: https://patentgrep.com/patent/US10479000B2

## CPC

- B29C 35/0805: {using electromagnetic radiation}
- B01L 3/502707: {characterised by the manufacture of the container or its components}
- B29C 35/0888: {using transparant moulds}
- B01L 2300/0816: Cards, e.g. flat sample carriers usually with flow in two horizontal directions
- B01L 2300/0819: Microarrays; Biochips
- B01L 2300/0822: Slides
- B29C 2035/0827: {using UV radiation}
- B29L 2031/752: {Measuring equipment}
- G01N 1/312: {for samples mounted on planar substrates}
- B29C: SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING (making preforms B29B11/00; making laminated products by combining previously unconnected layers which become one product whose layers will remain together B32B37/00 - B32B41/00)
- B01L: CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B29L: INDEXING SCHEME ASSOCIATED WITH SUBCLASS B29C, RELATING TO PARTICULAR ARTICLES
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)

## Abstract

According to the method for manufacturing a sample storage device and to the sample storage device of the present invention, a pattern forming mold having an engraved pattern part on the upper surface thereof is used to form a charging chamber sidewall on a first light-transmitting substrate, and a second light-transmitting substrate is adhered to the first light-transmitting substrate where the charging chamber sidewall is formed, and thus the sample storage device can be conveniently manufactured in large quantities.

## Claims

### Claim 1

A method for manufacturing a sample storage device having at least one charging chamber to count fine particles contained in a sample charged in the charging chamber, the method comprising:
preparing a pattern forming mold which has an engraved pattern part for forming at least a sidewall of the charging chamber, on an upper surface thereof, wherein the engraved pattern comprises a grid pattern, wherein the engraved pattern part include a sidewall pattern for forming the sidewall of the charging chamber, and a plurality of line patterns spaced apart from one another by a predetermined interval at a region outside the sidewall pattern;
pouring a predetermined amount of a liquid UV curable resin to be accommodated in the engraved pattern part, on the upper surface of the pattern forming mold;
seating a first light-transmitting substrate on the upper surface of the pattern forming mold and pressing them to each other;
radiating UV with the liquid UV curable resin accommodated in the engraved pattern part and thereby curing the liquid UV curable resin;
separating the first light-transmitting substrate which has the sidewall of the charging chamber formed as the liquid UV curable resin is cured, from the pattern forming mold; and
forming the charging chamber by adhering a second light-transmitting substrate to a surface of the first light-transmitting substrate on which the sidewall is formed,
wherein the second light-transmitting substrate has an introducing part and an discharging part, the introducing part is configured to receive the sample comprising the fine particles into the charging chamber, the discharging part is configured to permit the sample or air in the charging chamber to leave the charging chamber, and the introducing part and the discharging part span an entirety of a thickness of the second light-transmitting substrate, and
wherein the first light-transmitting substrate comprises a grid scale corresponding to the grid pattern of the forming mold.

### Claim 2

A method for manufacturing a sample storage device having at least one charging chamber to count fine particles contained in a sample charged in the charging chamber, the method comprising:
preparing a pattern forming mold which has an engraved pattern part for forming at least a sidewall of the charging chamber, on an upper surface thereof, the engraved pattern part being formed such that a plurality of pattern parts each of which forms a sidewall of a single charging chamber are arranged therein and thereby form sidewalls of a plurality of charging chambers, wherein the engraved pattern comprises at least one grid pattern, wherein the engraved pattern part include a sidewall pattern for forming the sidewall of the charging chamber, and a plurality of line patterns spaced apart from one another by a predetermined interval at a region outside the sidewall pattern;
pouring a predetermined amount of a liquid UV curable resin to be accommodated in the engraved pattern part, on the upper surface of the pattern forming mold;
seating a first light-transmitting base substrate on the upper surface of the pattern forming mold and pressing them to each other;
radiating UV with the liquid UV curable resin accommodated in the engraved pattern part and thereby curing the liquid UV curable resin;
separating the first light-transmitting base substrate which has the sidewalls of the plurality of charging chambers formed as the liquid UV curable resin is cured, from the pattern forming mold;
cutting the first light-transmitting base substrate such that the sidewalls of the plurality of charging chambers are divided into individual sidewalls of individual charging chambers and thereby defining first light-transmitting substrates; and
forming each charging chamber by adhering a second light-transmitting substrate to a surface of each first light-transmitting substrate on which the sidewall is formed,
wherein the second light-transmitting substrate has an introducing part and an discharging part, the introducing part is configured to receive the sample comprising the fine particles into the charging chamber, the discharging part is configured to permit the sample or air in the charging chamber to leave the charging chamber, and the introducing part and the discharging part span an entirety of a thickness of the second light-transmitting substrate, and
wherein the first light-transmitting substrate comprises at least one grid scale corresponding to the at least one grid pattern of the forming mold.