# US 10,478,990 B2 · Punching device with illuminated receiving plate > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 10,478,990 B2 - Document ID: US10478990B2 - Status: Granted - Kind: B2 - Application number: 14/113,937 - Filed: 2012-04-29 - Published: 2014-02-20 - Granted: 2019-11-19 - Earliest claimed: 2011-04-29 - Assignees: Hamilton Bonaduz AG - Applicants: Nando Giovanoli; Reto Menzi - Inventors: Nando Giovanoli; Reto Menzi - Web page: https://patentgrep.com/patent/US10478990B2 ## CPC - B26F 1/14: Punching tools; Punching dies - G01N 1/286: {involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising (microtomes G01N1/06; pulverising in general B02C; mixing in general B01F)} - G01N 35/028: {having reaction cells in the form of microtitration plates} - G01N 2001/288: {Filter punches} - Y10T 83/04: Processes - Y10T 83/828: With illuminating or viewing means for work - B26F: PERFORATING; PUNCHING; CUTTING-OUT; STAMPING-OUT; SEVERING BY MEANS OTHER THAN CUTTING (marking-out, perforating or making buttonholes A41H25/00; shoemaking A43D; surgery A61B; punching metal B21D; drilling metal B23B; cutting of metal by applying heat locally, e.g. flame cutting, B23K; cutting by abrasive fluid jets B24C5/02; details common to machines for severing B26D; drilling wood B27C; drilling stone B28D; working of plastics or substances in a plastic state B29; making boxes, cartons, envelopes or bags, of paper or similarly worked materials, e.g. metal foil, B31B; of glass C03B; of leather C14B; of textile materials D06H; for light guides G02B6/25; of tickets G07B) - G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q) - Y10T: TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION ## Abstract A punching device for processing samples applied to a sample card is provided. The punching device includes a movable gripper unit and an image capturing device and at least one punching head having a punch and a lower die. The punching head has a receiving opening into which a sample card is introducible by means of the movable gripper unit and is positionable relative to the punching head. The punching device further includes a punching drive which is couplable or coupled to the punch of the punching head. The punching device further includes a receiving plate supporting the receiving container and having a light source illuminating at least a part of the receiving plate. The light source is arranged such that at least a part of the receiving container located on the receiving plate can be illuminated from the direction of the receiving plate. The punching device further includes an image processing device configured to use data about positioning of the receiving container relative to the image capturing device to determine an analysis set of receiving recesses of the receiving container. ## Claims ### Claim 1 A punching device for processing samples applied to a sample card, the punching device comprising: a movable gripper unit; an image capturing device; at least one punching head having a punch and a lower die, wherein the punch is movable between a rest position in which it is away from the lower die and a punching position in which it is close to the lower die, and wherein the punching head has a receiving opening into which a sample card is introducible by means of the movable gripper unit and is positionable relative to the punching head; a punching drive which is couplable or coupled to the punch of the punching head and by way of which the movement of the punch between the rest position and the punching position is driven, wherein the punching head is set up such that a sample piece punched out of the sample card can be dispensed at an outlet opening of the lower die into a receiving recess in a receiving container arranged beneath the punching head; a receiving plate supporting the receiving container and having a light source illuminating at least a part of the receiving plate, wherein the light source is arranged such that at least a part of the receiving container located on the receiving plate can be illuminated from the direction of the receiving plate; and an image processing device configured to use data about positioning of the receiving container relative to the image capturing device to determine an analysis set of receiving recesses of the receiving container in an analysis region being a part of the receiving container, said analysis region being changeable by moving the receiving container relative to the image capturing device, wherein the image processing device is further configured to detect outlines of the receiving recesses in the analysis set and to determine the positions thereof, and is further configured to detect sample pieces contained in the receiving recesses in the analysis set. ### Claim 2 (depends on 1) The punching device as claimed in claim 1, wherein at least one electroluminescent (EL) film is provided as the light source on the receiving plate. ### Claim 3 (depends on 2) The punching device as claimed in claim 2, wherein the receiving plate is a transparent plate. ### Claim 4 (depends on 2) The punching device as claimed in claim 2, wherein the transparent plate is a glass plate. ### Claim 5 (depends on 2) The punching device as claimed in claim 2, wherein the EL film is arranged on the underside of the receiving plate. ### Claim 6 (depends on 1) The punching device as claimed in claim 1, wherein the image capturing device is arranged such that an article to be at least partially captured thereby can be arranged between an image plane of the image capturing device and the light source, such that the light source is located behind the article to be captured, as seen from an image capturing plane. ### Claim 7 (depends on 6) The punching device as claimed in claim 6, wherein the article to be at least partially captured is one of the receiving container located on the receiving plate, or receiving recesses provided in said receiving container. ### Claim 8 (depends on 1) The punching device as claimed in claim 1, further comprising a transport frame resting on the receiving plate and able to move in the plate plane, at least one receiving container being receivable in said transport frame. ### Claim 9 (depends on 8) The punching device as claimed in claim 8, wherein the receiving plate forms a cover of a housing for a drive unit which enables the movement of the transport frame on the receiving plate. ### Claim 10 (depends on 9) The punching device as claimed in claim 9, wherein the transport frame is coupled in a contactless manner by means of magnets to the drive unit covered by the receiving plate. ### Claim 11 (depends on 8) The punching device as claimed in claim 8, wherein the gripper unit is configured such that receiving containers can be moved toward and away from the transport frame by means of the gripper unit. ### Claim 12 (depends on 1) The punching device as claimed in claim 1, wherein the gripper unit comprises a sample card gripper and a receiving container gripper, wherein receiving containers are transportable by means of the gripper unit from waiting positions outside the receiving plate to processing positions on the receiving plate and vice versa. ### Claim 13 (depends on 1) The punching device as claimed in claim 1, wherein the light source is arranged such that receiving recesses provided in said receiving container can be illuminated from the direction of the receiving plate. ### Claim 14 (depends on 1) The punching device as claimed in claim 1, wherein the light source is arranged such that at least the part of the receiving container that can be illuminated can be illuminated from below. ### Claim 15 (depends on 1) The punching device as claimed in claim 1, further comprising a monitor and wherein the analysis set can be displayed on the monitor. ### Claim 16 A method for evaluating at least one result of a punching operation comprising the steps of: punching at least one sample piece out of at least one sample card by means of a punching head; receiving the at least one sample piece in a receiving container; illuminating the receiving container or the punched sample card by means of a light source; capturing an image of the receiving container or of the punched sample card by means of an image capturing device, wherein the receiving container or the punched sample card is arranged between the light source and an image capturing plane of the image capturing device such that light from the light source which reaches the image capturing device passes through translucent portions of the receiving container or of the sample card; determining an analysis set of receiving recesses of the receiving container using data about positioning of the receiving container relative to the image capturing device in an analysis region being a part of the receiving container, said analysis region being changeable by moving the receiving container relative to the image capturing device; detecting outlines of the receiving recesses in the analysis set and determining the positions thereof; and detecting sample pieces contained in the receiving recesses in the analysis set. ### Claim 17 (depends on 16) The method as claimed in claim 16, further comprising establishing at which points of the image light from the light source passes through or is covered by evaluating an image captured by the image capturing device. ### Claim 18 (depends on 16) The method as claimed in claim 16, further comprising evaluating an image of the receiving container as to whether at least one receiving recess of the receiving container contains at least one sample piece punched out of a sample card, said sample piece at least partially preventing light from the light source from passing through. ### Claim 19 (depends on 16) The method as claimed in claim 16, further comprising evaluating an image of the sample card as to at which points of the sample card punched-out openings, through which the light from the light source passes, are present. ### Claim 20 (depends on 16) The method as claimed in claim 16, wherein the light source emits light in the visible wave range. ### Claim 21 (depends on 20) The method as claimed in claim 20, wherein light emitted in the visible wave range is emitted at a wavelength which corresponds to one of blue, green, or red. ### Claim 22 (depends on 16) The method as claimed in claim 16, further comprising comparing the result of an evaluation of a captured image with already known comparative values in order to check the correct progress of the punching operation. ### Claim 23 (depends on 22) The method as claimed in claim 22, wherein the check is made as to whether a previously punched-out sample piece is contained in a predetermined receiving recess or/and whether the punching out has taken place at a previously determined point of the sample card. ### Claim 24 (depends on 1, 16) The method as claimed in claim 16, wherein the punching device of claim 1 is used to perform the method steps. ### Claim 25 (depends on 16) The method as claimed in claim 16, wherein the at least one sample piece is received in a receiving recess of the receiving container. ### Claim 26 (depends on 16) The method as claimed in claim 16, further comprising moving the receiving container relative to the image capturing device. ### Claim 27 (depends on 16) The method as claimed in claim 16, further comprising checking an assignment of sample pieces to particular receiving recesses. ### Claim 28 (depends on 16) The method as claimed in claim 16, further comprising displaying an indication of empty receiving recesses in the receiving set. ### Claim 29 (depends on 16) The method as claimed in claim 16, further comprising checking for the presence of a punched hole in the at least one sample card.