# US 10,478,822 B1 · Automated cell processing instruments comprising reagent cartridges

> Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt

- Number: US 10,478,822 B1
- Document ID: US10478822B1
- Status: Granted
- Kind: B1
- Application number: 16/525,152
- Filed: 2019-07-29
- Published: 2019-11-21
- Granted: 2019-11-19
- Earliest claimed: 2018-04-13
- Assignees: Inscripta, Inc.
- Applicants: Inscripta, Inc.
- Inventors: Don Masquelier; Phillip Belgrader; Brian Van Hatten; Jorge Bernate; Bruce Chabansky
- Web page: https://patentgrep.com/patent/US10478822B1

## CPC

- B01L 3/527: {for a plurality of reagents}
- B01L 7/00: Heating or cooling apparatus (autoclaves B01J3/04); Heat insulating devices
- B01L 7/02: Water baths; Sand baths; Air baths
- C12M 23/06: {Tubular (C12M23/08, C12M23/16 take precedence)}
- C12M 23/44: {Multiple separable units; Modules}
- C12M 29/04: {Filters; Permeable or porous membranes or plates, e.g. dialysis}
- C12M 35/02: {Electrical or electromagnetic means, e.g. for electroporation or for cell fusion}
- C12M 41/18: {Heat exchange systems, e.g. heat jackets or outer envelopes}
- C12M 41/48: {Automatic or computerized control (automatic analysis G01N35/00)}
- C12N 13/00: Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
- B01L 2200/028: Modular arrangements
- B01L 2200/16: Reagents, handling or storing thereof
- B01L 2300/04: Closures and closing means
- B01L 2300/042: Caps; Plugs
- B01L 2300/0645: Electrodes
- B01L 2300/0829: Multi-well plates; Microtitration plates
- B01L 2300/12: Specific details about materials
- B01L: CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- C12M: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; {APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS}
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)

## Abstract

The present disclosure provides a reagent cartridge configured for use in an automated multi-module cell processing environment.

## Claims

### Claim 1

An automated multi-module cell processing instrument comprising:
a housing;
a reagent cartridge, wherein the reagent cartridge comprises:
a thermal block with block reservoirs defined therein;
a cover disposed upon the thermal block with cover reservoirs defined therein, wherein the cover reservoirs and block reservoirs are coincident and together define reservoirs in the reagent cartridge;
an insert comprising a transformation module, wherein the transformation module is a flow-through electroporation device;
a script readable by a processor;
at least two additional inserts configured to reside within the reservoirs in the reagent cartridge; and
reagents dispensed in one or more of the reservoirs;
a growth module;
a cell concentration module;
an editing module; and
the processor.

### Claim 2 (depends on 1)

The automated multi-module cell processing instrument of claim 1, wherein the one or more reservoirs with reagents dispensed therein is sealed with a foil seal.

### Claim 3 (depends on 1)

The automated multi-module cell processing instrument of claim 1, further comprising a top cover disposed upon the cover wherein the top cover is configured to seal the reservoirs.

### Claim 4 (depends on 1)

The automated multi-module cell processing instrument of claim 1, wherein the growth module comprises a rotating growth vial with media and cells dispensed therein.

### Claim 5 (depends on 4)

The automated multi-module cell processing instrument of claim 4, wherein the cells are bacterial cells.

### Claim 6 (depends on 5)

The automated multi-module cell processing instrument of claim 5, wherein the cells are E. coli cells.

### Claim 7 (depends on 4)

The automated multi-module cell processing instrument of claim 4, wherein the cells are yeast cells.

### Claim 8 (depends on 7)

The automated multi-module cell processing instrument of claim 7, wherein the cells are S. cerevisiae cells.

### Claim 9 (depends on 4)

The automated multi-module cell processing instrument of claim 4, wherein the cells are plant cells.

### Claim 10 (depends on 4)

The automated multi-module cell processing instrument of claim 4, wherein the cells are animal cells.

### Claim 11 (depends on 10)

The automated multi-module cell processing instrument of claim 10, wherein the cells are mammalian cells.

### Claim 12 (depends on 1)

The automated multi-module cell processing instrument of claim 1, wherein one of the reagents dispensed in the one or more reservoirs comprises an antibiotic.

### Claim 13 (depends on 1)

The automated multi-module cell processing instrument of claim 1, wherein one of the reagents dispensed in the one or more reservoirs comprises a nucleic acid-guided nuclease.

### Claim 14 (depends on 13)

The automated multi-module cell processing instrument of claim 13, wherein the nucleic acid-guided nuclease is MAD7.

### Claim 15 (depends on 1)

The automated multi-module cell processing instrument of claim 1, wherein the editing module is configured to singulate cells.

### Claim 16

An automated multi-module cell processing instrument comprising:
a housing;
a reagent cartridge for an automated multi-module cell processing instrument, wherein the reagent cartridge comprises:
a thermal block with block reservoirs defined therein;
a cover disposed upon the thermal block with cover reservoirs defined therein, wherein the cover reservoirs and block reservoirs are coincident and together define reservoirs in the reagent cartridge;
an insert comprising a transformation module;
a script readable by a processor;
at least two additional inserts configured to reside within the reservoirs in the reagent cartridge; and
reagents dispensed in one or more of the reservoirs;
a growth module;
a cell concentration module;
a singulation and editing module; and
the processor.

### Claim 17 (depends on 16)

The automated multi-module cell processing instrument of claim 16, wherein the singulation and editing module is a solid wall device.

### Claim 18 (depends on 16)

The automated multi-module cell processing instrument of claim 16, wherein the transformation module in the reagent cartridge is a flow-through electroporation device.

### Claim 19

An automated multi-module cell processing instrument comprising:
a housing;
a reagent cartridge for an automated multi-module cell processing instrument, wherein the reagent cartridge comprises:
a thermal block with block reservoirs defined therein;
a cover disposed upon the thermal block with cover reservoirs defined therein, wherein the cover reservoirs and block reservoirs are coincident and together define reservoirs in the reagent cartridge;
an insert comprising a transformation module;
a script readable by a processor;
at least two additional inserts configured to reside within the reservoirs in the reagent cartridge; and
reagents dispensed in one or more of the reservoirs;
a growth module;
a cell concentration module; and
the processor.

### Claim 20 (depends on 19)

The automated multi-module cell processing instrument of claim 19, wherein the transformation module in the reagent cartridge comprises a flow-through electroporation device.