# US 10,478,638 B2 · Recombinant self-assembling protein comprising target-oriented peptide and use thereof > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 10,478,638 B2 - Document ID: US10478638B2 - Status: Granted - Kind: B2 - Application number: 15/782,234 - Filed: 2017-10-12 - Published: 2018-02-01 - Granted: 2019-11-19 - Earliest claimed: 2015-09-09 - Assignees: Korea University Research and Business Foundation - Applicants: Korea University Research and Business Foundation - Inventors: Jeewon Lee; Koo-Chul Kwon - Web page: https://patentgrep.com/patent/US10478638B2 ## CPC - A61N 5/062: {Photodynamic therapy, i.e. excitation of an agent} - A61K 41/0052: {Thermotherapy; Hyperthermia; Magnetic induction; Induction heating therapy} - A61K 47/62: the modifying agent being a protein, peptide or polyamino acid - A61K 49/0428: {Surface-modified nanoparticles, e.g. immuno-nanoparticles} - C07K 14/005: from viruses - C07K 14/47: from mammals - C07K 14/71: for growth factors; for growth regulators - C07K 14/78: Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin or cold insoluble globulin [CIG] - C07K 2319/00: Fusion polypeptide - C07K 2319/20: containing a tag with affinity for a non-protein ligand - C07K 2319/21: containing a His-tag - C07K 2319/32: fusions with soluble part of a cell surface receptor, "decoy receptors" - C07K 2319/33: fusions for targeting to specific cell types, e.g. tissue specific targeting, targeting of a bacterial subspecies - C07K 2319/735: containing a domain for self-assembly, e.g. a viral coat protein (includes phage display) - C12N 2730/10122: New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes - C12N 2730/10123: Virus like particles [VLP] - C12N 2730/10133: Use of viral protein as therapeutic agent other than vaccine, e.g. apoptosis inducing or anti-inflammatory - C12N 2730/10142: virus or viral particle as vehicle, e.g. encapsulating small organic molecule - C12N 2810/855: from receptors; from cell surface antigens; from cell surface determinants - A61N: ELECTROTHERAPY; MAGNETOTHERAPY; RADIATION THERAPY; ULTRASOUND THERAPY (measurement of bioelectric currents A61B; surgical instruments, devices or methods for transferring non-mechanical forms of energy to or from the body A61B18/00; anaesthetic apparatus in general A61M; incandescent lamps H01K; infrared radiators for heating H05B) - A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D) - C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00) - C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00) ## Abstract The present invention relates to a recombinant self-assembled protein comprising a target-oriented peptide and a use thereof The recombinant self-assembled protein according to the present invention, comprising a target-oriented peptide, does not require an additional process for providing target-orientedness, and is thus capable of delivering a desired drug to a target tissue or target cell without using additives, such as chemical binders or stabilizers; therefore, the protein can be used for photothermal therapy, drug delivery, imaging, or the like. In particular, according to the present invention, it is possible to prepare gold-protein nanoparticle fusions in which uniform high-density gold nanoparticles having target-orientedness are bound to protein surfaces, without an additional process of surface stabilization or process for providing target-orientedness. Compared with conventional gold nanoparticles, the gold-protein nanoparticle fusions according to the present invention show structural stability against pH variation and concentration variation, and also have excellent target-orientedness; therefore, the fusions can bring a dramatic enhancement to the utilization of gold nanoparticles in photothermal therapy. ## Claims ### Claim 1 A recombinant self-assembled protein, comprising a target-oriented peptide fused to a self-assembled protein, wherein the target-oriented peptide is a cancer-targeting peptide ligand. ### Claim 2 (depends on 1) The recombinant self-assembled protein of claim 1, wherein the self-assembled protein is ferritin or hepatitis B virus (HBV) capsid protein. ### Claim 3 (depends on 1) A recombinant self-assembled protein nanoparticle, comprising the recombinant self-assembled protein of claim 1. ### Claim 4 (depends on 3) The recombinant self-assembled protein nanoparticle of claim 3, wherein the cancer-targeting peptide ligand is exposed on a surface of the recombinant self-assembled protein nanoparticle.