# US 10,478,587 B2 · Device and method for preparing tissue, particularly adipose tissue

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- Number: US 10,478,587 B2
- Document ID: US10478587B2
- Status: Granted
- Kind: B2
- Application number: 14/700,186
- Filed: 2015-04-30
- Published: 2015-08-20
- Granted: 2019-11-19
- Earliest claimed: 2010-05-20
- Assignees: Lipogems International S.P.A.
- Applicants: Lipogems International S.P.A.
- Inventors: Carlo Tremolada
- Web page: https://patentgrep.com/patent/US10478587B2

## CPC

- A61M 1/88: {Draining devices having means for processing the drained fluid, e.g. an absorber (for liposuction A61M1/892)}
- A61B 17/34: Trocars; Puncturing needles
- A61K 35/35: Fat tissue; Adipocytes; Stromal cells; Connective tissues (adipose-derived stem cells A61K35/28; collagen A61K38/39)
- A61M 1/815: {the barrel serving as aspiration container, e.g. in a breast pump}
- A61M 1/892: {with treatment of the collected fat}
- A61M 1/895: {with means for reinjection of collected fat}
- A61M 5/002: {Packages specially adapted therefor, e.g. packages for syringes or needles or kits for diabetics}
- A61M 5/315: Pistons; Piston-rods; Guiding, blocking or restricting the movement of the rod {or piston}; Appliances on the rod for facilitating dosing {; Dosing mechanisms}
- A61M 5/3286: {Needle tip design, e.g. for improved penetration}
- A61M 19/00: Local anaesthesia (syringes therefor A61M5/00); Hypothermia (A61M5/42 takes precedence; cooling blood in a bypass of the arterial system A61M1/36)
- A61P 3/00: Drugs for disorders of the metabolism (of the blood or the extracellular fluid A61P7/00)
- A61P 17/00: Drugs for dermatological disorders
- B02C 19/0056: {specially adapted for specific materials not otherwise provided for}
- B08B 3/102: {with means for agitating the liquid (by agitating the container B08B3/042, B08B3/044, B08B3/045, B08B3/06)}
- C12M 45/02: {by mechanical forces; Stirring; Trituration; Comminuting (crushing, pulverizing, disintegrating in general B02C)}
- C12M 47/04: {Cell isolation or sorting (purging biological preparations of unwanted cells C12N5/0081, determining the presence or kind of microorganism C12Q1/04)}
- C12N 5/0653: {Adipocytes; Adipose tissue}
- A61M 2202/08: Lipoids
- A61M 2202/09: Body tissue
- A61M: DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY (introducing media into or onto the bodies of animals A61D7/00; means for inserting tampons A61F13/26; devices for administering food or medicines orally A61J; containers for collecting, storing or administering blood or medical fluids A61J1/05); DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY (surgery A61B; chemical aspects of surgical articles A61L); DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR {(Electrotherapy, e.g. producing anaesthesia by the use of alternating or intermittent currents A61N1/36021)}
- A61B: DIAGNOSIS; SURGERY; IDENTIFICATION (usefulness limited to only animals A61D)
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- B02C: CRUSHING, PULVERISING, OR DISINTEGRATING IN GENERAL; MILLING GRAIN ({household tools and machines for pulverising foodstuffs, e.g. coffee and spice mills A47J42/00; pharmaceutical mortars A61J3/02; mechanical processing of refuse and garbage B03B9/06; dressing mould materials by grinding B22C5/04}; obtaining metallic powder by crushing, grinding or milling B22F9/04; {recovery of plastics by disintegrating B29B17/00; crushing raw materials in starch making C08B30/02; beaters for papermaking D21D1/02; crushing devices specially for transport in mines E21F13/002; slag crushing devices F23J1/00; fuel milling devices in combustion apparatus F23K1/00; household devices for crushing coal F24B15/02; ice disintegrating devices F25C5/02})
- B08B: CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- C12M: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; {APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS}
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)

## Abstract

A device and a method for preparing adipose tissue for transplantation from lobular fat extracted, for instance by liposuction, the fat including a fluid component that have an oily component, a blood component and/or sterile solutions, and a solid component including cell fragments, cells and one or more cell macroagglomerates of heterogeneous size, wherein the device includes at least one washing and separating container having a washing chamber, the container has an inlet and an outlet for the liposuctioned material to enter the washing chamber through the inlet and for at least part of the material to exit the washing chamber through the outlet, the washing chamber including stirring means for forming an emulsion of fluid components.

## Claims

### Claim 1

A method of treating injuries or diseases in a patient comprising:
extracting lipoaspirate from donor areas of a first patient;
treating said lipoaspirate with a size reducing means for reducing the size of cell magroagglomerates of the lipoaspirate to averagely equal smaller cell agglomerates, wherein said size reducing means consist of at least one series of parallel or intersecting sheets or cutting wires, to form at least one size reducing net, through which the lipoaspirate is passed; and
injecting the treated material into a second patient,
wherein the step of injecting the treated material into the second patient is carried out by introducing a needle or cannula, and wherein the step of introducing is performed several times to create a 3D net of tunnels in subcutaneous or muscular tissue, with very small amounts of cell material being introduced therein.

### Claim 2 (depends on 1)

The method as claimed in claim 1, wherein the first patient and the second patient are a same person.

### Claim 3 (depends on 1)

The method as claimed in claim 1, wherein the step of injecting is performed using a blunted-end sterile cannula having a diameter of less than 1 mm.

### Claim 4 (depends on 1)

The method as claimed in claim 1, wherein the step of injecting is performed using a pointed-end, blunted-end, helical, or spiral cannula (5), said cannula being introduced and extracted by a rotary motion, thereby allowing the cannula, and hence the material, to enter bodily tissue by a spiral motion.

### Claim 5

A method of treating injuries or diseases in a patient comprising:
extracting biological material from donor areas of a first patient;
treating said biological material; and
injecting the treated material into a second patient,
wherein the treating step comprises one or more of a size reducing step of the extracted biological material or the step of washing and separating a liquid phase from a solid cell phase, said treating step being carried out using the following method:
injecting a sterile washing solution into a washing chamber (101) of a washing and separating container (1), said washing chamber (101) containing at least one stirring element (104) having a shape and size relative to the washing chamber (101) that allows an emulsion of fluid components when said washing and separating container (1) is subjected to stirring, a manual stirring action being sufficient;
stirring said washing and separating container (1) to facilitate the emulsion of the fluid components with an emulsion-forming system (104);
arranging said washing and separating container (1) in a position that provides a stratification of the solid components on the emulsion, which constitutes fat contained in the washing chamber (101); and
injecting a clean washing solution through an inlet (102), discharging the emulsion of fluid components from the washing chamber (101) through an outlet (103) of the washing and separating container (1), and removing the emulsion of the fluid components by density gradient;
wherein the step of injecting the treated material into the second patient is carried out by introducing a needle or cannula, and wherein the step of introducing is performed several times to create a 3D net of tunnels in subcutaneous or muscular tissue, with very small amounts of cell material being introduced therein.

### Claim 6 (depends on 5)

The method as claimed in claim 5, wherein the first patient and the second patient are a same person.

### Claim 7 (depends on 5)

The method as claimed in claim 5, wherein the treated and injected material is other than adipose tissue.