# US 10,478,526 B2 · Skin substitutes and methods for hair follicle neogenesis

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- Number: US 10,478,526 B2
- Document ID: US10478526B2
- Status: Granted
- Kind: B2
- Application number: 14/888,613
- Filed: 2014-05-01
- Published: 2016-06-30
- Granted: 2019-11-19
- Earliest claimed: 2013-05-03
- Assignees: The Henry M. Jackson Foundation for the Advancement of Military Medicine, Inc.
- Applicants: The Henry M. Jackson Foundation for the Advancement of Military Medicine, Inc.
- Inventors: Rajesh Thangapazham; Thomas N. Darling; Shaowei Li
- Web page: https://patentgrep.com/patent/US10478526B2

## CPC

- A61L 27/3886: {comprising two or more cell types}
- A61L 27/24: Collagen
- A61L 27/3804: {characterised by specific cells or progenitors thereof, e.g. fibroblasts, connective tissue cells, kidney cells}
- A61L 27/3813: {Epithelial cells, e.g. keratinocytes, urothelial cells}
- A61L 27/60: Materials for use in artificial skin
- A61P 17/00: Drugs for dermatological disorders
- A61P 17/02: for treating wounds, ulcers, burns, scars, keloids, or the like
- A61P 17/14: for baldness or alopecia
- A61L 2430/18: for hair reconstruction
- A61L: METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES (preservation of bodies or disinfecting characterised by the agents employed A01N; preserving, e.g. sterilising, food or foodstuffs A23; preparations for medical, dental or toiletry purposes A61K)
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS

## Abstract

This invention provides compositions in the form of skin substitutes comprising epithelial cells and mesenchymal cells, wherein the mesenchymal cells are not isolated from the occipital or nape region of the scalp, as well as methods for using the same.

## Claims

### Claim 1

A skin substitute comprising, in a suspension,
(i) epithelial cells; and
(ii) isolated mesenchymal cells that are derived from the temporal region of a scalp, wherein the skin substitute induces hair follicle neogenesis.

### Claim 2 (depends on 1)

The skin substitute of claim 1, wherein the skin substitute is provided in a microsphere.

### Claim 3 (depends on 1)

The skin substitute of claim 1, wherein the suspension comprises a matrix.

### Claim 4 (depends on 3)

The skin substitute of claim 3, wherein the matrix is a collagen matrix.

### Claim 5 (depends on 1)

The skin substitute of claim 1, wherein the isolated mesenchymal cells are hair follicle dermal cells.

### Claim 6 (depends on 5)

The skin substitute of claim 5, wherein the hair follicle dermal cells are neural crest-derived cells, dermal papilla cells, or dermal sheath cells.

### Claim 7 (depends on 1)

The skin substitute of claim 1, wherein the epithelial cells are keratinocytes.

### Claim 8 (depends on 1)

The skin substitute of claim 1, wherein the epithelial cells are from a first or second passage and/or the isolated mesenchymal cells are from a first, second, third, or fourth passage.

### Claim 9 (depends on 1)

The skin substitute of claim 1, wherein the epithelial cells and the isolated mesenchymal cells are passaged in keratinocyte-conditioned medium.

### Claim 10 (depends on 1)

The skin substitute of claim 1, wherein the epithelial cells and the isolated mesenchymal cells are human.

### Claim 11 (depends on 1)

The skin substitute of claim 1, wherein the isolated mesenchymal cells are genetically unmodified mesenchymal cells.

### Claim 12 (depends on 1)

A method for inducing hair follicle neogenesis, comprising delivering the skin substitute of claim 1 to a human subject, wherein the human subject has partial-thickness skin loss, full-thickness skin loss, a wound, a burn, a scar, or hair loss.