# US 10,478,485 B2 · Methods and compositions to prevent or treat bacterial infections

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- Number: US 10,478,485 B2
- Document ID: US10478485B2
- Status: Granted
- Kind: B2
- Application number: 15/741,069
- Filed: 2016-07-01
- Published: 2018-07-12
- Granted: 2019-11-19
- Earliest claimed: 2015-07-01
- Assignees: Board of Regents, The University of Texas System
- Applicants: Patrick Ketter; Bernard Arulanandam; Neal Guentzel
- Inventors: Patrick Ketter; Bernard Arulanandam; Neal Guentzel
- Web page: https://patentgrep.com/patent/US10478485B2

## CPC

- A61K 39/104: {Pseudomonadales, e.g.} Pseudomonas
- A61K 39/05: {Actinobacteria, e.g. Actinomyces, Streptomyces, Nocardia, Bifidobacterium, Gardnerella}, Corynebacterium; Propionibacterium {(Mycobacterium A61K39/04)}
- A61P 31/04: Antibacterial agents
- C12N 1/20: Bacteria; Culture media therefor
- C12N 1/36: Adaptation or attenuation of cells
- C12N 9/0036: {acting on NADH or NADPH (1.6)}
- C12N 9/0051: {acting on a sulfur group of donors (1.8)}
- C12Y 108/01009: Thioredoxin-disulfide reductase (1.8.1.9), i.e. thioredoxin-reductase
- A61K 2039/522: {avirulent or attenuated}
- A61K 2039/54: {characterised by the route of administration}
- A61K 2039/575: {humoral response}
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- C12Y: ENZYMES

## Abstract

Certain embodiments are directed to methods and compositions for preventing treating bacterial infections. In certain embodiments the compositions comprise thioredoxin deficient bacteria.

## Claims

### Claim 1

An Acinetobacter baumanni mutant having a full or partial deletion in the thioredoxin-A (TrxA) gene that results in a non-functional TrxA gene.

### Claim 2

A vaccine comprising a live attenuated Acinetobacter baumanni, wherein the attenuated Acinetobacter baumanni is Acinetobacter baumanni having a full or partial deletion in the thioredoxin-A (TrxA) gene that results in a non-functional TrxA gene and induces an immune response to Acinetobacter baumanni in a subject in need thereof.

### Claim 3 (depends on 2)

The vaccine of claim 2, wherein the subject is domesticated or a livestock animal.

### Claim 4 (depends on 2)

The vaccine of claim 2, wherein the vaccine is formulated for oral administration.

### Claim 5 (depends on 2)

The vaccine of claim 2, wherein the vaccine is formulated for vaccination against Acinetobacter baumanni.

### Claim 6

A method of inducing an immune response against Acinetobacter baumanni in a subject in need thereof comprising administering to said subject an effective dose of a live attenuated Acinetobacter baumanni, wherein the attenuated Acinetobacter baumanni is Acinetobacter baumanni having a full or partial deletion in the thioredoxin-A (TrxA) gene that results in a non-functional TrxA gene.

### Claim 7 (depends on 6)

The method of claim 6, wherein the attenuated Acinetobacter baumanni is administered orally.

### Claim 8 (depends on 6)

The method of claim 6, wherein the subject is domesticated or a livestock animal.