# US 10,478,479 B2 · Method for preparing dendritic cell, dendritic cell prepared thereby, and use thereof

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- Number: US 10,478,479 B2
- Document ID: US10478479B2
- Status: Granted
- Kind: B2
- Application number: 15/327,933
- Filed: 2015-07-28
- Published: 2017-07-13
- Granted: 2019-11-19
- Earliest claimed: 2014-08-01
- Assignees: Jw Creagene Inc.
- Applicants: Jw Creagene Inc.
- Inventors: Yoon Lee; Young-Mok Kim; So-Yeon Kim; Seung-Soo Han; Yong-Soo Bae
- Web page: https://patentgrep.com/patent/US10478479B2

## CPC

- C12N 5/0639: {Dendritic cells, e.g. Langherhans cells in the epidermis}
- A61K 35/12: Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells (vaccines or medicinal preparations containing antigens or antibodies A61K39/00)
- A61K 40/19: Dendritic cells
- A61K 40/24: Antigen-presenting cells [APC]
- A61K 40/42: Cancer antigens
- C12N 2501/02: Compounds of the arachidonic acid pathway, e.g. prostaglandins, leukotrienes
- C12N 2501/2301: Interleukin-1 (IL-1)
- C12N 2501/2306: Interleukin-6 (IL-6)
- C12N 2501/24: Interferons [IFN]
- C12N 2501/25: Tumour necrosing factors [TNF]
- C12N 2501/727: Kinases (EC 2.7.)
- C12N 2501/999: Small molecules not provided for elsewhere
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)

## Abstract

The present invention relates to a method for preparing a dendritic cell, a dendritic cell prepared thereby and a use thereof, and more specifically, to a method for preparing a dendritic cell, including: treating a dendritic cell at a maturation stage rather than at an immature stage with an antigen bound to a peptide having a cell membrane permeability to prepare a dendritic cell with improved antigen-presenting ability, a dendritic cell prepared by the method, and an immunotherapeutic agent thereof, a use for anti-tumor vaccines, or a pharmaceutical composition for treating tumors, containing the same.

## Claims

### Claim 1

A method for preparing a dendritic cell, comprising:
culturing an immature dendritic cell to a mature dendritic cell with treating by 1-10 ng/mL of rapamycin and a maturation factor; and
after maturation of the immature dendritic cell to the mature dendritic cell has been completed, sensitizing the mature dendritic cell with an antigen bound to a peptide having cell permeability,
wherein said sensitizing of the mature dendritic cell with the antigen bound to the peptide having cell permeability is performed at least 6 hours and up to 48 hours after said treating by the maturation factor, and
wherein said sensitizing of the mature dendritic cell with the antigen bound to the peptide having cell permeability is performed for 1 to 8 hours.

### Claim 2 (depends on 1)

The method according to claim 1, wherein the peptide having cell permeability comprises a cytoplasmic transduction peptide (CTP).

### Claim 3 (depends on 1)

The method according to claim 1, wherein the mature dendritic cell is sensitized in said sensitizing with the antigen bound to the peptide having cell permeability, so that an expression level of CD80, CD83, or CD40 is increased by 60% or more, compared to that of the immature dendritic cell.