# US 10,478,472 B2 · Combination of tolerogenic peptides and TFG-B for use in the induction and maintenance of oral tolerance in young mammals

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- Number: US 10,478,472 B2
- Document ID: US10478472B2
- Status: Granted
- Kind: B2
- Application number: 15/580,180
- Filed: 2016-06-10
- Published: 2018-05-24
- Granted: 2019-11-19
- Earliest claimed: 2015-06-12
- Assignees: Societe des Produits Nestle S.A.
- Applicants: Nestec S.A.
- Inventors: Sophie Helene Nutten; Nanda De Groot; Valerie Francoise Verhasselt
- Web page: https://patentgrep.com/patent/US10478472B2

## CPC

- A61K 38/1841: {Transforming growth factor [TGF]}
- A23K 20/147: Polymeric derivatives, e.g. peptides or proteins
- A23K 50/40: for carnivorous animals, e.g. cats or dogs
- A23K 50/60: for weanlings
- A23L 33/17: Amino acids, peptides or proteins
- A23L 33/18: Peptides; Protein hydrolysates
- A23L 33/40: {Complete food formulations for specific consumer groups or specific purposes, e.g. infant formula}
- A61K 38/018: {from milk}
- A61P 37/08: Antiallergic agents (antiasthmatic agents A61P11/06; ophthalmic antiallergics A61P27/14)
- A23V 2002/00: Food compositions, function of food ingredients or processes for food or foodstuffs
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- A23K: FODDER
- A23L: FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF (preservation thereof A23B)
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A23V: INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION

## Abstract

This invention relates to the administration of a combination of TGF-β and one or more peptides comprising specific sequences of β-lacto-globulin. The peptides are hypoallergenic but retain the capability to induce tolerance to the native protein. The combination of TGF-β and one or more of these peptides has the therapeutic effect of inducing and maintaining oral tolerance towards newly introduced proteins, in particular, at the weaning period. The combination can modulate the infant and toddler immune response to allergens.

## Claims

### Claim 1

A method for induction and maintenance of oral tolerance to β-lactoglobulin (BLG) and maintenance of oral tolerance to other food proteins that has been acquired via consumption of maternal mill in a young mammal during and after weaning period, the method comprising administering to the young mammal a composition comprising at least eight different peptides; each having a length from five to twelve amino adds that comprises a sequence selected from SEQ ID No. 1-8, and the composition further comprising transforming growth factor-beta (TGF-β), wherein each of SEQ ID NOs: 1-8 is present in the composition.

### Claim 2

A method for primary prevention of allergic reactions to milk proteins and to electedly introduced dietary protein in a young mammal during and after weaning, the method comprising administering to the young mammal a composition comprising at least eight different peptides, each having a length from five to twelve amino adds that comprises a sequence selected from SEQ ID No. 1-8, and the composition further comprising TGF-β-β), wherein each of SEQ NOs: 1-8 is present in the composition.

### Claim 3 (depends on 1)

The method according to claim 1, wherein the young mammal is a human infant or toddler.

### Claim 4 (depends on 1)

The method according to claim 1, wherein the young mammal has been fed maternal milk from a mother who has been exposed to an ingredient selected from the group consisting of milk, egg, nut, peanut, shellfish, wheat, soy, and any other food protein during the feeding period.

### Claim 5 (depends on 1)

The method according to claim 1, wherein the young mammal is at risk of developing allergy.

### Claim 6 (depends on 1)

The method according to claim 1, wherein the young mammal is a companion animal.

### Claim 7 (depends on 2)

The method according to claim 2, wherein the young mammal is a human infant or toddler.

### Claim 8 (depends on 2)

The method according to claim 2, wherein the young mammal has been fed maternal milk from a mother who has been exposed to an ingredient selected from the group consisting of milk, egg, nut, peanut, shellfish, wheat, soy, and any other food protein during the feeding period.

### Claim 9 (depends on 2)

The method according to claim 2, wherein the young mammal is at risk of developing allergy.

### Claim 10 (depends on 2)

The method according to claim 2, wherein the young mammal is a companion animal.