# US 10,478,468 B2 · Method for enhancing effect of immunotherapy for cancer

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- Number: US 10,478,468 B2
- Document ID: US10478468B2
- Status: Granted
- Kind: B2
- Application number: 15/808,534
- Filed: 2017-11-09
- Published: 2018-05-10
- Granted: 2019-11-19
- Earliest claimed: 2016-11-10
- Assignees: Phytohealth Corporation
- Applicants: Phytohealth Corporation
- Inventors: Liang-Shun Wang; Chi-Tai Yeh; Chun-Hung Wu; Yi-Li Lee
- Web page: https://patentgrep.com/patent/US10478468B2

## CPC

- A61K 36/481: Astragalus (milkvetch)
- A61K 45/06: Mixtures of active ingredients without chemical characterisation, e.g. antiphlogistics and cardiaca
- A61P 1/00: Drugs for disorders of the alimentary tract or the digestive system
- A61P 11/06: Antiasthmatics
- A61P 35/00: Antineoplastic agents
- A61P 35/04: specific for metastasis
- A61P 37/02: Immunomodulators
- A61P 37/04: Immunostimulants
- A61P 37/08: Antiallergic agents (antiasthmatic agents A61P11/06; ophthalmic antiallergics A61P27/14)
- A61P 43/00: Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS

## Abstract

Disclosed is a method for enhancing the effect of an immunotherapy for cancer in a subject, comprising administering to the subject an effective amount of a polysaccharide extract of Astragalus membranaceus. Also disclosed is a method for increasing a subject's pool of M1 macrophages, wherein the subject is suffering from one or more conditions associated with undesirable M2 macrophage activation, the method comprising administering to the subject an effective amount of a polysaccharide extract of Astragalus membranaceus.

## Claims

### Claim 1

A method for increasing a subject's pool of M1 macrophages, wherein the subject is suffering from one or more conditions associated with undesirable M2 macrophage activation, the method comprising
administering to the subject an effective amount of a polysaccharide extract of Astragalus membranaceus, said polysaccharide extract comprising an arabinose:galactose ratio ranging from about 1.5:1 to about 3:1; from about 5% to about 15% arabinose; less than about 1.5% rhamnose; from about 3% to about 7% galactose; less than about 4% galacturonic acid; and, from about 70% to about 90% glucose.

### Claim 2 (depends on 1)

The method of claim 1, wherein the one or more conditions associated with undesirable M2 activation comprise cancer, asthma, allergy, or a progressive fibrosis disease.

### Claim 3 (depends on 1)

The method of claim 1, wherein the polysaccharide extract has an average molecular weight ranging from about 20 kDa to about 60 kDa.

### Claim 4 (depends on 1)

The method of claim 1, wherein the method reduces at least one of the following: (i) a macrophage pro-tumoral function; (ii) tumor-associated macrophage recruitment into tumor; (iii) tumor invasion and metastasis; (iv) tumor angiogenesis; (v) tumor growth; and (vi) tumor cell proliferation.