# US 10,477,793 B2 · Tomato line FIRKL13-4132

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- Number: US 10,477,793 B2
- Document ID: US10477793B2
- Status: Granted
- Kind: B2
- Application number: 15/939,125
- Filed: 2018-03-28
- Published: 2018-10-11
- Granted: 2019-11-19
- Earliest claimed: 2015-05-08
- Assignees: Seminis Vegetable Seeds, Inc.
- Applicants: Seminis Vegetable Seeds, Inc.
- Inventors: Bernardus van den Bosch
- Web page: https://patentgrep.com/patent/US10477793B2

## CPC

- A01H 6/825: {Solanum lycopersicum [tomato]}
- A01H 5/08: Fruits
- A01H: NEW PLANTS OR {NON-TRANSGENIC} PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES

## Abstract

The invention provides seed and plants of tomato line FIRKL13-4132. The invention thus relates to the plants, seeds, and tissue cultures of line FIRKL13-4132 and to methods for producing a tomato plant produced by crossing such plants with themselves or with another tomato plant, such as a plant of another genotype. The invention further relates to seeds and plants produced by such crossing. The invention further relates to parts of such plants, including the fruit and gametes of such plants.

## Claims

### Claim 1

A tomato plant comprising at least a first set of the chromosomes of tomato line FIRKL13-4132, a sample of seed of said line having been deposited under ATCC Accession Number PTA-122113.

### Claim 2 (depends on 1)

A tomato seed that produces the plant of claim 1.

### Claim 3 (depends on 1)

A plant part of the plant of claim 1, wherein said plant part comprises a cell of said plant.

### Claim 4 (depends on 1)

A tissue culture of regenerable cells of the plant of claim 1.

### Claim 5 (depends on 1)

A method of vegetatively propagating the tomato plant of claim 1, the method comprising the steps of:
(a) collecting tissue capable of being propagated from the plant according to claim 1; and
(b) propagating a tomato plant from said tissue.

### Claim 6 (depends on 1)

A method of introducing a trait into a tomato line, the method comprising:
(a) utilizing as a recurrent parent the plant of claim 1 by crossing said plant with a donor tomato plant that comprises a trait to produce F1 progeny;
(b) selecting an F1 progeny that comprises the trait;
(c) backcrossing the selected F1 progeny with a plant of the same tomato line used as the recurrent parent in step (a) to produce backcross progeny;
(d) selecting a backcross progeny comprising the trait; and
(e) repeating steps (c) and (d) three or more times to produce a selected fourth or higher backcross progeny that comprises the trait and otherwise comprises all of the physiological and morphological characteristics of tomato line FIRKL13-4132.

### Claim 7 (depends on 6)

A tomato plant produced by the method of claim 6.

### Claim 8 (depends on 1)

A method of producing a tomato plant comprising an added trait, the method comprising introducing by genetic transformation a transgene conferring the trait into the plant of claim 1.

### Claim 9 (depends on 8)

A tomato plant produced by the method of claim 8, wherein said plant comprises the trait and otherwise comprises all of the morphological and physiological characteristics of tomato line FIRKL13-4132.

### Claim 10

A tomato plant comprising at least a first set of the chromosomes of tomato line FIRKL13-4132, a sample of seed of said line having been deposited under ATCC Accession Number PTA-122113, further comprising a transgene.

### Claim 11 (depends on 10)

The plant of claim 10, wherein the transgene confers a trait selected from the group consisting of male sterility, herbicide tolerance, insect resistance, pest resistance, disease resistance, modified fatty acid metabolism, environmental stress tolerance, modified carbohydrate metabolism and modified protein metabolism.

### Claim 12

A tomato plant comprising at least a first set of the chromosomes of tomato line FIRKL13-4132, a sample of seed of said line having been deposited under ATCC Accession Number PTA-122113, further comprising a single locus conversion.

### Claim 13 (depends on 12)

The plant of claim 12, wherein the single locus conversion confers a trait selected from the group consisting of male sterility, herbicide tolerance, insect resistance, pest resistance, disease resistance, modified fatty acid metabolism, environmental stress tolerance, modified carbohydrate metabolism and modified protein metabolism.

### Claim 14 (depends on 1)

A method for producing a seed of a tomato plant derived from tomato line FIRKL13-4132, the method comprising the steps of:
(a) crossing the tomato plant of claim 1 with itself or a different tomato plant; and
(b) allowing a seed of a line FIRKL13-4132-derived tomato plant to form.

### Claim 15 (depends on 1)

A method of producing a seed of a line FIRKL13-4132-derived tomato plant, the method comprising the steps of:
(a) producing a line FIRKL13-4132-derived tomato plant from a seed produced by crossing the tomato plant of claim 1 with itself or a different tomato plant; and
(b) crossing the line FIRKL13-4132-derived tomato plant with itself or a different tomato plant to obtain a seed of a further line FIRKL13-4132-derived tomato plant.

### Claim 16 (depends on 15)

The method of claim 15, further comprising repeating said producing and crossing steps of (a) and (b) using the seed from said step (b) for at least one generation to produce a seed of an additional line FIRKL13-4132-derived tomato plant.

### Claim 17 (depends on 1)

A method of producing a tomato fruit, the method comprising:
(a) obtaining the plant according to claim 1, wherein the plant has been cultivated to maturity; and
(b) collecting a tomato fruit from the plant.
