# US 10,477,791 B2 · Method of production of phytocannabinoids for use in medical treatments > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 10,477,791 B2 - Document ID: US10477791B2 - Status: Granted - Kind: B2 - Application number: 16/290,708 - Filed: 2019-03-01 - Published: 2019-06-27 - Granted: 2019-11-19 - Earliest claimed: 2017-10-25 - Assignees: Cell Science Holding Ltd - Applicants: Cell Science Holding Ltd - Inventors: Peter Andrew Whitton - Web page: https://patentgrep.com/patent/US10477791B2 ## CPC - A01H 4/005: {Methods for micropropagation; Vegetative plant propagation using cell or tissue culture techniques} - A01H 3/02: by controlling duration, wavelength, intensity, or periodicity of illumination - A01H 6/28: Cannabaceae, e.g. cannabis - A61K 31/658: {o-phenolic cannabinoids, e.g. cannabidiol, cannabigerolic acid, cannabichromene or tetrahydrocannabinol} - A61K 36/3482: {Cannabis} - C12N 5/04: Plant cells or tissues {(culture media C12N5/0025)} - A01H: NEW PLANTS OR {NON-TRANSGENIC} PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES - A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D) - C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00) ## Abstract A method of producing cannabinoids for use in medical treatments by growing cultured Cannabis sativa plant cells through tissue culture, the method comprising the steps of: selecting Cannabis sativa leaf tissue for culture; and growing a tissue culture from the selected leaf tissue in a liquid based medium whilst controlling the light exposure of the tissue culture to control the cannabinoid content of the tissue culture. Control of the light exposure can enable the phytocannabinoid content of the grown tissue culture to be tailored to the use intended for the tissue culture. For example, the THC content of the tissue culture can be controlled to be maximised or minimised depending on the intended use. Use of tissue culture is beneficial as compared to prior art methods as it allows for genetic consistency and reduces the resources necessary to produce plant cells containing phytocannabinoids. ## Claims ### Claim 1 A method of producing one or more cannabinoids from Cannabis plant cells, the method comprising the steps of: selecting a Cannabis tissue for culture; growing a cell suspension culture from the selected Cannabis tissue in a liquid based medium whilst controlling the light exposure of the cell suspension culture to control the content of the one or more cannabinoids produced by the cell suspension culture, the light exposure comprising UV light; and extracting the one or more cannabinoids from the cell suspension culture. ### Claim 2 (depends on 1) A method according to claim 1, wherein the light exposure is controlled such that the cell suspension culture is constantly exposed to PAR during growth of the cell suspension culture. ### Claim 3 (depends on 2) A method according to claim 2, wherein the PAR is controlled to provide at least 0.2 moles of photons per day. ### Claim 4 (depends on 2) A method according to claim 2, wherein the PAR is controlled to provide 0.5 moles of photons per day. ### Claim 5 (depends on 1) A method according to claim 1, wherein the light exposure is controlled such that the cell suspension culture is exposed to UVA light during growth of the cell suspension culture. ### Claim 6 (depends on 1) A method according to claim 1, wherein the light exposure is controlled such that cell suspension culture is exposed to UVB light during growth of the cell suspension culture. ### Claim 7 (depends on 1) A method according to claim 1, wherein the UV light is controlled during growth of the cell suspension culture such that the cell suspension culture is exposed to UV light of an intensity greater or equal to 1200 lumens but less than 2000 lumens and the UV light exposure is cycled through alternating periods of exposure and darkness; wherein each period of exposure is at least 30 minutes and each period of darkness is at least 30 minutes. ### Claim 8 (depends on 7) A method according to claim 7, wherein each period of exposure is equal to or less than one hour and each period of darkness is equal to or less than one hour. ### Claim 9 (depends on 7) A method according to claim 7, wherein the intensity of the UV light is less than or equal to 2000 lumens. ### Claim 10 (depends on 1) A method according to claim 1, wherein the UV light is controlled during growth of the cell suspension culture such that the cell suspension culture is constantly exposed to UV light of an intensity equal to or less than 1200 lumens. ### Claim 11 (depends on 9) A method according to claim 9, wherein the UV light has an intensity equal to or less than 600 lumens. ### Claim 12 (depends on 1) A method according to claim 1, wherein during growing the cell suspension culture is maintained at a temperature between 25° C. and 30° C. ### Claim 13 (depends on 12) A method according to claim 12, wherein during growing the cell suspension culture is maintained at a temperature of 27° C. ### Claim 14 (depends on 1) A method according to claim 1, wherein the cell suspension culture is grown for between 10 and 28 days. ### Claim 15 (depends on 14) A method according to claim 14, wherein the cell suspension culture is grown for 14 days. ### Claim 16 (depends on 1) A method according to claim 1, wherein the cell suspension culture is agitated during growth of the cell suspension culture. ### Claim 17 (depends on 1) A method according to claim 1, wherein the CO2 content of the environment in which the cell suspension culture is grown is controlled to increase cell suspension culture. ### Claim 18 (depends on 1) A method according to claim 1, comprising the further step of collecting and freeze-drying the cell suspension culture after growing. ### Claim 19 (depends on 1) A method of claim 1 wherein the selected the Cannabis tissue was previously grown in a liquid based medium whilst controlling the light exposure of the cell suspension culture to control the content of the one or more cannabinoids of the cell suspension culture. ### Claim 20 (depends on 1) A method of claim 1, wherein the one or more cannabinoids comprises THC.