# US 10,477,787 B2 · Method to identify asian soybean rust resistance quantitative trait loci in soybean and compositions thereof > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 10,477,787 B2 - Document ID: US10477787B2 - Status: Granted - Kind: B2 - Application number: 15/614,151 - Filed: 2017-06-05 - Published: 2017-09-28 - Granted: 2019-11-19 - Earliest claimed: 2008-04-24 - Assignees: Monsanto Technology LLC - Applicants: Monsanto Technology LLC - Inventors: George J. Baley; Vergel C. Concibido; Bradley J. La Vallee - Web page: https://patentgrep.com/patent/US10477787B2 ## CPC - A01H 1/1255: {for fungal resistance} - A01H 1/045: {using molecular markers} - A01H 4/00: Plant reproduction by tissue culture techniques {; Tissue culture techniques therefor} - A01H 5/10: Seeds - A01H 6/542: {Glycine max [soybean]} - C12Q 1/6895: for plants, fungi or algae - C12Q 2600/13: Plant traits - C12Q 2600/156: Polymorphic or mutational markers - C12Q 2600/158: Expression markers - A01H: NEW PLANTS OR {NON-TRANSGENIC} PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES - C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES ## Abstract The present invention is in the field of plant breeding and disease resistance. More specifically, the invention includes a method for breeding soybean plants containing quantitative trait loci that are associated with resistance to Asian Soybean Rust (ASR), a fungal disease associated with Phakopsora spp. The invention further includes germplasm and the use of germplasm containing quantitative trait loci (QTL) conferring disease resistance for introgression into elite germplasm in a breeding program for resistance to ASR. ## Claims ### Claim 1 A method for producing an Asian Soybean Rust (ASR) resistant soybean plant, said method comprising: selecting a soybean plant using marker assisted selection from a plurality of soybean plants derived from an ASR resistant soybean plant comprising ASR resistance locus 16 by detecting a molecular marker that is within 10 centimorgans or less from a resistance allele of a SNP marker selected from the group consisting of NS0113966 and NS0118536; obtaining a seed from said selected plant, and generating from said obtained seed an ASR-resistant progeny plant comprising said resistance allele. ### Claim 2 (depends on 1) The method of claim 1, wherein said molecular marker is mapped to within 5 centimorgans or less from said resistance allele of a SNP marker selected from the group consisting of NS0113966 and NS0118536. ### Claim 3 (depends on 1) The method of claim 1, wherein said molecular marker is mapped to within 1 centimorgan or less from said resistance allele of a SNP marker selected from the group consisting of NS0113966 and NS0118536. ### Claim 4 (depends on 1) The method of claim 1, wherein said molecular marker is selected from the group consisting of NS0113966 and NS0118536. ### Claim 5 (depends on 1) The method of claim 1, wherein said progeny plant comprises a resistance allele having a sequence selected from the group consisting of SEQ ID NOs: 79 and 80. ### Claim 6 (depends on 1) The method of claim 1, wherein said progeny plant exhibits at least partial resistance to at least one race of an ASR-inducing fungus. ### Claim 7 (depends on 1) The method of claim 1, wherein said progeny plant is resistant to Phakopsora pachyrhizi or Phakopsora meibomiae. ### Claim 8 (depends on 1) The method of claim 1, wherein said progeny plant seed further comprises one or more traits selected from the group consisting of herbicide tolerance, increased yield, insect control, fungal disease resistance, virus resistance, nematode resistance, bacterial disease resistance, mycoplasma disease resistance, modified oils production, high oil production, high protein production, germination and seedling growth control, enhanced animal and human nutrition, lower raffinose, environmental stress resistance, increased digestibility, production of industrial enzymes, production of pharmaceutical proteins, production of pharmaceutical peptides, production of pharmaceutical small molecules, improved processing traits, improved flavor, improved nitrogen fixation, improved hybrid seed production, reduced allergenicity, and improved production of biopolymers and biofuels. ### Claim 9 (depends on 1) The method of claim 1, wherein the ASR resistance of said progeny plant is derived from ASR resistance locus 16. ### Claim 10 A method of creating a population of soybean plants having Asian Soybean Rust (ASR) resistance locus 16, said method comprising: genotyping one or more soybean plants from a first population containing said ASR resistance locus 16 for a molecular marker within 10 centimorgans or less from a resistance allele of a SNP marker selected from the group consisting of NS0113966 and NS0118536; selecting from said one or more soybean plants at least one soybean plant identified as containing said ASR resistance locus 16; and producing from said at least one soybean plant a second population, thereby creating a population of soybean plants comprising said ASR resistance locus 16. ### Claim 11 (depends on 10) The method of claim 10, wherein said ASR resistance locus 16 is obtainable from PI507009. ### Claim 12 (depends on 10) The method of claim 10, wherein said ASR resistance locus 16 is identifiable by one or more of the markers selected from the group consisting of NS0113966 and NS0118536. ### Claim 13 (depends on 10) The method of claim 10, wherein said method comprises crossing a soybean plant comprising ASR resistance locus 16 to an elite soybean plant to produce an ASR resistant elite soybean plant population. ### Claim 14 (depends on 10) The method of claim 10, wherein said molecular marker is detected using an assay selected from the group consisting of single base extension (SBE), allele-specific primer extension sequencing (ASPE), DNA sequencing, RNA sequencing, microarray-based analyses, universal PCR, allele specific extension, hybridization, mass spectrometry, ligation, extension-ligation, and Flap Endonuclease-mediated assay. ### Claim 15 (depends on 10) The method of claim 10, wherein said molecular marker is mapped to within 5 centimorgans or less from said SNP marker selected from the group consisting of NS0113966 and NS0118536. ### Claim 16 (depends on 10) The method of claim 10, wherein said molecular marker is mapped to within 1 centimorgan or less from said SNP marker selected from the group consisting of NS0113966 and NS0118536. ### Claim 17 (depends on 10) The method of claim 10, wherein said molecular marker is selected from the group consisting of NS0113966 and NS0118536. ### Claim 18 (depends on 10) The method of claim 10, wherein said second population comprises a resistance allele having a sequence selected from the group consisting of SEQ ID NOs: 79 and 80. ### Claim 19 (depends on 1) The method of claim 1, wherein said molecular marker is between said SNP marker NS0113966 and said SNP marker NS0118536. ### Claim 20 (depends on 10) The method of claim 10, wherein said molecular marker is between said SNP marker NS0113966 and said SNP marker NS0118536.