Device for imaging a sample with detection of an asymmetrically distributed angular range

A method for imaging a sample arranged in an object plane, in which the sample is illuminated with a light sheet, wherein the light sheet lies essentially in the object plane and defines an illumination direction, and the normal of the object plane defines a detection direction, an area of the sample is imaged from the object plane by means of an optical relay system into an intermediate image plane, wherein the object plane and the intermediate image plane with an optical axis of the relay system include an angle different from 90°, the intermediate image plane is imaged by means of an optical imaging system with an objective, the optical axis of which lies perpendicularly on the intermediate image plane and which is focused on the intermediate image plane, undistorted onto a detector and is recorded there as an image, wherein the optical imaging system includes an aperture which enables detection of a detection angular range distributed asymmetrically around the optical axis, the recorded image is processed in an evaluation unit connected to the detector while taking into account an asymmetrical point spread function on the basis of the asymmetrical detection angular spectrum and/or a compressed point spread function.