Method of determination of risk of 2 hour blood glucose equal to or greater than 140 mg/dL

A method of treating and/or preventing diabetes or a related cardio-diabetes condition and comorbidity in a subject, comprising:

• administering to the subject a drug or supplement in an amount effective to treat diabetes or the related cardio-diabetes condition and comorbidity, wherein the subject has been determined to have abnormal glucose tolerance or to be at risk for developing abnormal glucose tolerance by:
• a) obtaining a biological sample from the subject;
• b) processing the biological sample by one of the following:
• i) concentrating the biological sample to obtain a concentrated biological sample;
• ii) fractionating the biological sample to obtain a fractionated biological sample;
• or
• iii) separating the biological sample to obtain a separated biological sample;
• c) obtaining, from the concentrated biological sample, the fractionated biological sample, or the separated biological sample, a measured level of a combination of biomarkers selected from the group consisting of, free fatty acids, glucose, myeloperoxidase, insulin, fibrinogen, and leptin, that predict and/or provide information about at least three physiological processes selected from the group consisting of, adipose tissue insulin resistance, pancreatic beta cell dysfunction and/or exhaustion, muscle and/or hepatic insulin resistance, functional enhancement of insulin secretion by beta cells, inhibition of beta cell function, adipokine function, total glycemic control, and inflammation,
• d) calculating a score based on the measured levels of the combination of biomarkers, wherein the score calculation comprises:
• ln
• [
• FFA
• 2.374
• ×
• Glucose
• 5.248
• ×
• MPO
• 1.7415
• ×
• Insulin
• 1.037
• ×
• Fibrinogen
• 3.037
• Leptin
• 0.601
• ]
• e) comparing the score to reference values from a population of randomly chosen subjects, with or without normal glucose tolerance, and
• f) identifying the subject as likely to develop abnormal glucose tolerance if the calculated score is elevated, wherein an elevated score correlates with an increased likelihood of elevation of blood glucose to ≥140 mg/dL at 2 hours after oral glucose tolerance test.