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US 10,473,907 B2Granted
Microscopic examination of an object using a sequence of optical microscopy and particle beam microscopy
AssigneeCarl Zeiss Microscopy GmbH
InventorsChristian Thomas, Torsten Sievers, Alexander Thesen
Life of this patent
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Abstract
A slide system for optical microscopy using immersion fluid including a cover glass having a first refractive index and a transparent film having a second refractive index. The second refractive index of the transparent film is matched to the first refractive index of the cover glass. The transparent film protects the cover glass from immersion fluid used for optical microscopy and the transparent film is configured such that the immersion fluid used can be removed by lifting off the transparent film.
Claims6
A method for microscopy of an object, comprising
- using a slide system comprising a slide and a cover glass having a first refractive index, a transparent film having a second refractive index, the second refractive index of the transparent film being matched to the first refractive index of the cover glass;
- and
- protecting the cover glass from immersion fluid used for optical microscopy by application of the transparent film wherein the immersion fluid is removable by lifting off the transparent film;
- imaging the object with one of an optical microscope and a particle beam microscope;
- and
- removing the object from the one of the optical microscope and the particle beam microscope and imaging it with the other of the optical microscope and the particle beam microscope.
3 more elements
Dependencies are inferred from the claim text.