Microscope
AssigneeNikon Corporation
InventorsYoshitaro Nakano, Takaaki Okamoto
Life of this patent
Estimating the term…
Abstract
A microscope device includes an interface detector that detects the position of an interface present in a container containing an observation object and a controller that performs focus maintenance control for maintaining the focal position of an objective lens in a reference position distant from the interface detected by the interface detector in the direction of the optical path of the objective lens by a predetermined distance (an offset value) and, in a time period when an image capture unit is capturing images, changes the focal position from the reference position by moving at least one of the objective lens and observation object in the direction of the optical path with reference to the reference position.
Claims10
A microscope comprising:
- a first optical system that collects a first light at a predetermined position in an observation object;
- a second optical system that collects a second light at a predetermined position in a container containing the observation object;
- a detector that detects a light from the container;
- a controller;
- and
- an image capture unit, wherein,
- while moving a stage for holding the observation object in a direction perpendicular to an optical axis of an objective lens, the controller performs focus maintenance control for maintaining a focal position of the first light in a position distant by a first distance from the predetermined position in the container by moving at least one of the stage and the objective lens in a direction of the optical axis of the objective lens on the basis of the light detected by the detector, and
- the controller moves the focal position of the first light to a plurality of positions by moving at least one of the stage and the objective lens in the direction of the optical axis of the objective lens, acquires a plurality of images by causing the image capture unit to capture images of the observation object, and causes the image capture unit to capture an image of the observation object at a position distant by a second distance from the predetermined position in the container by moving at least one of the stage and the objective lens in the direction of the optical axis of the objective lens on the basis of the images.
A microscope comprising:
- a first optical system that collects a first light at a predetermined position in an observation object;
- a second optical system that collects a second light at a predetermined position in a container containing the observation object;
- a detector that detects a light from the container;
- a controller;
- and
- an image capture unit, wherein,
- while waiting for elapsing of a predetermined time interval in a method of capturing an image of the observation object at the predetermined time interval, the controller performs focus maintenance control for maintaining a focal position of the first light in a position distant by a first distance from the predetermined position in the container by moving at least one of the stage and the objective lens in a direction of the optical axis of the objective lens on the basis of the light detected by the detector, and
- after the predetermined time interval is elapsed, the controller moves the focal position of the first light to a plurality of positions by moving at least one of the stage and the objective lens in the direction of the optical axis of the objective lens, acquires a plurality of images by causing the image capture unit to capture images of the observation object, and causes the image capture unit to capture an image of the observation object at a position distant by a second distance from the predetermined position in the container by moving at least one of the stage and the objective lens in the direction of the optical axis of the objective lens on the basis of the images.
Dependencies are inferred from the claim text.